LOCUS       sequence-120762-         753 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of P. leioghnati LUMP protein, a FRET acceptor 
            protein emitting a cyan-colored fluorescence with an unusually long 
            excited state lifetime.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 753)
  AUTHORS   Hoepker AC, Wang A, Le Marois A, Suhling K, Yan Y, Marriott G
  TITLE     Genetically encoded sensors of protein hydrodynamics and molecular 
            proximity.
  JOURNAL   Proc Natl Acad Sci U S A. 2015 Apr 30. pii: 201424021.
  PUBMED    25931526
REFERENCE   2  (bases 1 to 753)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2015 Apr 30. pii: 201424021."
COMMENT     Sequence Label: 130_pSKB3-LUMP-P.leioghnati
FEATURES             Location/Qualifiers
     source          1..753
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(16..33)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(613..633)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(655..672)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             691..713
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    728..752
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 agcagccgga tctcagtggt ggtggtggtg gtgctcgagt gcggccgcct accattcatt
       61 taaaatttca acgttcactt tcacatcttt tgctaacgat gcaatgttag tactcgctaa
      121 caaatcacgc gagtaattca atgtaataat gctgtctgac atatcagtga taggcaaaga
      181 gatcccatca attccaatat catctttcac tgttaagttt tcagctaacc ctttagggat
      241 atcaatcagt acagaaaaac ctgcgtcatt ctcttctatt gctgcgacca gcgcagtgcc
      301 tttaatattt cctgttaatc caccacggcc aaccacttca ccaaatttag ggtggatctc
      361 taggtttacc tgatcgcctt cttttaatcc atcaaatgtt gtcgtaccca atgcttggtc
      421 gatatcaaag tacaccatat cgcctaaaat acggacaact gtcaatgaac aaccatttac
      481 taacattaca gtatctacat caacaagttg aaacataccc tcagggaatg caatcccatg
      541 tcgctgacta tcttcacttt ttgaaatgga tcgaataaca ccacgacctt gaacaatacc
      601 tctaaacata tggccctgga agtaaaggtt ttcggtcgtt gggatatcgt aatcgtgatg
      661 atgatgatga tggctgctgc ccatggtata tctccttctt aaagttaaac aaaattattt
      721 ctagagggga attgttatcc gctcacaatt ccc
//
