LOCUS       sequence-120798-         946 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  human VPS33A ORF was inserted into pMXsIP SPGFP-GS Ci2 vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 946)
  AUTHORS   Jiang P, Nishimura T, Sakamaki Y, Itakura E, Hatta T, Natsume T, 
            Mizushima N
  TITLE     The HOPS complex mediates autophagosome-lysosome fusion through 
            interaction with syntaxin 17.
  JOURNAL   Mol Biol Cell. 2014 Apr;25(8):1327-37. doi: 10.1091/mbc.E13-08-0447.
            Epub 2014 Feb 19.
  PUBMED    24554770
REFERENCE   2  (bases 1 to 946)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1091/mbc.E13-08-0447"; journalName: "Mol Biol Cell"; date: 
            "2014-04"; volume: "25"; issue: "8"; pages: "1327-37"
COMMENT     Sequence Label: pMXsIP GFP-VPS33A
FEATURES             Location/Qualifiers
     source          1..946
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(36..57)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(297..316)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     644..665
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa
       61 acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga
      121 ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca
      181 ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact
      241 tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg
      301 acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca
      361 tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt
      421 acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg
      481 tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc
      541 agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca
      601 cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt
      661 tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagggt ggcggtggct
      721 cgggcggtgg tgggtcgggt ggcggcggat ctatcgatga attgaattca cgcgttcgcg
      781 agttaacgga tccctcgagc atggcggctc atctgtccta cggccgagtg aacctaaacg
      841 tgttgcgcga ggcggtgcgt cccgagctgc gcgagttcct ggacaagtgc gcaggaagca
      901 aggcaatagt ttgggatgaa tacctaactg gaccctttgg cctgat
//
