LOCUS       sequence-120832-         453 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses ELP[V-150] in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 453)
  AUTHORS   Tang NC, Chilkoti A
  TITLE     Combinatorial codon scrambling enables scalable gene synthesis and 
            amplification of repetitive proteins.
  JOURNAL   Nat Mater. 2016 Jan 4. doi: 10.1038/nmat4521.
  PUBMED    26726995
REFERENCE   2  (bases 1 to 453)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Mater. 
            2016 Jan 4. doi: 10.1038/nmat4521."
COMMENT     Sequence Label: pET-24a-ELP[V-150]
FEATURES             Location/Qualifiers
     source          1..453
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     58..77
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        58..76
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    77..101
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             117..137
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             149..166
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaattaa
       61 tacgactcac tataggggaa ttgtgagcgg ataacaattc ccctctagaa ataattttgt
      121 ttaactttaa gaaggaggag tacatatgca tcatcatcat catcacagtt cagggggtgt
      181 aggtgtccct ggagtaggcg ttccaggcgt cggggtacct ggcgtgggtg ttcccggcgt
      241 tggagtcccg ggtgtaggag tccccggtgt tggtgtacct ggagtgggag taccgggagt
      301 aggagtacca ggagtggggg ttcctggtgt gggagtccca ggagtcggtg taccaggggt
      361 aggggtaccg ggcgtaggtg tgccaggagt tggggtgcct ggagtcngag tccctggtgt
      421 cggagtacct ggtgttggag tacccggagt tgg
//
