LOCUS       sequence-120845-         816 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MBII85 snoRNA expression cassett, contains snoRNA in natural intron 
            sorounded by natural exons. To improve snoRNA expression efficiency 
            5' and 3' splice sites were mutated to consensus sequences..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 816)
  AUTHORS   Falaleeva M, Surface J, Shen M, de la Grange P, Stamm S
  TITLE     SNORD116 and SNORD115 change expression of multiple genes and modify
            each other's activity.
  JOURNAL   Gene. 2015 Jul 26. pii: S0378-1119(15)00848-3. doi: 
            10.1016/j.gene.2015.07.023.
  PUBMED    26220404
REFERENCE   2  (bases 1 to 816)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene. 2015 
            Jul 26. pii: S0378-1119(15)00848-3. doi: 
            10.1016/j.gene.2015.07.023."
COMMENT     Sequence Label: pcDNA3.1(+)MBII-85-5'/3'ss mut
FEATURES             Location/Qualifiers
     source          1..816
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     28..47
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        28..46
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 acccactgct tactggctta tcgaaattaa tacgactcac tatagggaga cccaagctgg
       61 ctagcgttta aacttaagct tggtaccggt tgcattccct ttccagtatg gtccatggaa
      121 aacatgcaga ggaaatggcc ccacagcatt ggctgagtgt ttatgtcgtc caactgagtc
      181 tccatcgaac tgttgtggtg ttgctgactt gccctaggtc acctgagtta aaaggtaagt
      241 tatatgggaa ttgctgcatg aattctaggt gcccaggtct tggtggagca gggaacagct
      301 gccattgatt atgtccaggc ccttcgggac atggagcatg cctagggcct ctggtttctc
      361 ccatgttttg agctttggcc cattgttccc tggcagtgga ttgtcagtgt ccagcagtcc
      421 attgctctcc ccaaagccag agcactccag agtactggaa ccacttaatg caatgcactc
      481 caacgagctt ggatctatga tgattcccag tcaaacattc cttggaaaag ctgaacaaaa
      541 tgagtgaaaa ctctgtaccg ccactctcat cggaactgag gtccagcatc ttgggtctcc
      601 caggtgctat gggcgtcagg cacaggctgc ccaaacactc cattgttctt gacttcttcg
      661 ggctctagcc agactctcca gagacaggtg tgtgcttggc ctactctgcc aaaaattcat
      721 ggtgtaggac acagcaggtc cttaagcact ggtggcttgc tccctttgct gtagtggaga
      781 tgcttggcag atactcagta ggtagtcatg ctaggt
//
