LOCUS       sequence-120867-        1042 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  A pCS2 based vector to generate a 3'-Flag tag using GATEWAY cloning 
            technology.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1042)
  AUTHORS   Grant IM, Balcha D, Hao T, Shen Y, Trivedi P, Patrushev I, Fortriede
            JD, Karpinka JB, Liu L, Zorn AM, Stukenberg PT, Hill DE, Gilchrist 
            MJ
  TITLE     The Xenopus ORFeome: A resource that enables functional genomics.
  JOURNAL   Dev Biol. 2015 Dec 15;408(2):345-57. doi: 
            10.1016/j.ydbio.2015.09.004. Epub 2015 Sep 29.
  PUBMED    26391338
REFERENCE   2  (bases 1 to 1042)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ydbio.2015.09.004"; journalName: "Dev Biol"; date: 
            "2015-12-15- 15"; volume: "408"; issue: "2"; pages: "345-57"
COMMENT     Sequence Label: pCSF107mT-GATEWAY-3'-FLAG
FEATURES             Location/Qualifiers
     source          1..1042
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(103..109,110..127,128..133))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(158..282)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(347..365)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(348..365)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 gccattggga tatatcaacg gtggtatatc cagtgatttt tttctccatt ttagcttcct
       61 tagctcctga aaatctcgac ggatcctaac tcaaaatcca cacattatac gagccggaag
      121 cataaagtgt aaagcctggg gtgcctaatg cggccgccat agtgactgga tatgttgtgt
      181 tttacagtat tatgtagtct gttttttatg caaaatctaa tttaatatat tgatatttat
      241 atcattttac gtttctcgtt cagctttttt gtacaaactt gtggtaccaa gagatctgaa
      301 ttcttcgaaa tcgatggatc ctgcaaaaag aacaagtagc ttgtattcta tagtgtcacc
      361 taaatcaagc ttgctccgac gtccccaggc agaatggcgg ttccctaaac gagcattgct
      421 tatatagacc tcccattagg cacgcctacc gcccatttac gtcaatggaa cgcccatttg
      481 cgtcattgcc cctccccatt gacgtcaatg gggatgtact tggcagccat cgcgggccat
      541 ttaccgccat tgacgtcaat gggagtactg ccaatgtacc ctggcgtact tccaatagta
      601 atgtacttgc caagttacta ttaatagata ttgatgtact gccaagtggg ccatttaccg
      661 tcattgacgt caataggggg cgtgagaacg gatatgaatg ggcaatgagc catcccattg
      721 acgtcaatgg tgggtggtcc tattgacgtc aatgggcatt gagccaggcg ggccatttac
      781 cgtaattgac gtcaatgggg gaggcgccat atacgtcaat aggaccgccc atatgacgtc
      841 aataggaaag accatatata gagaccattg acgtcaatgg gggagtggct atgggcggta
      901 ttaggaagcc ccatatatgg tatatgggac cgcccattgg gaggggctat ctacgtcaat
      961 aggaaaaccc atatatggaa tactatatgg catagggcca atacatagta ttgaacctgg
     1021 ccaatagcca tattggcata gg
//
