LOCUS       sequence-120879-         765 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 765)
  AUTHORS   Tucker KL, Beard C, Dausmann J, Jackson-Grusby L, Laird PW, Lei H, 
            Li E, Jaenisch R
  TITLE     Germ-line passage is required for establishment of methylation and 
            expression patterns of imprinted but not of nonimprinted genes.
  JOURNAL   Genes Dev. 1996 Apr 15. 10(8):1008-20.
  PUBMED    8608936
REFERENCE   2  (bases 1 to 765)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes Dev. 
            1996 Apr 15. 10(8):1008-20."
COMMENT     Sequence Label: pPGKpuro
FEATURES             Location/Qualifiers
     source          1..765
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        55..554
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(90..113)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     487..506
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     complement(594..613)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
ORIGIN
        1 gggcgaattg gccaagtcgg ccgagctcga attcgtcgac ctcgaaattc taccgggtag
       61 gggaggcgct tttcccaagg cagtctggag catgcgcttt agcagccccg ctgggcactt
      121 ggcgctacac aagtggcctc tggcctcgca cacattccac atccaccggt aggcgccaac
      181 cggctccgtt ctttggtggc cccttcgcgc caccttctac tcctccccta gtcaggaagt
      241 tcccccccgc cccgcagctc gcgtcgtgca ggacgtgaca aatggaagta gcacgtctca
      301 ctagtctcgt gcagatggac agcaccgctg agcaatggaa gcgggtaggc ctttggggca
      361 gcggccaata gcagctttgc tccttcgctt tctgggctca gaggctggga aggggtgggt
      421 ccgggggcgg gctcaggggc gggctcaggg gcggggcggg cgcccgaagg tcctccggag
      481 gcccggcatt ctgcacgctt caaaagcgca cgtctgccgc gctgttctcc tcttcctcat
      541 ctccgggcct ttcgacctgc atccatctag atctcgagca gctgaagctt accatgaccg
      601 agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc
      661 tcgccgccgc gttcgccgac taccccgcca cgcgccacac cgtcgatccg gaccgccaca
      721 tcgagcgggt caccgagctg caagaactct tcctcacgcg cgtcg
//
