LOCUS       sequence-120914-         863 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  URA3 vector for cloning guide sequences using BclI-SwaI sites into 
            guide RNA expression cassette.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 863)
  AUTHORS   Laughery MF, Hunter T, Brown A, Hoopes J, Ostbye T, Shumaker T, 
            Wyrick JJ
  TITLE     New vectors for simple and streamlined CRISPR-Cas9 genome editing in
            Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2015 Dec;32(12):711-20. doi: 10.1002/yea.3098. Epub 2015 Sep 
            21.
  PUBMED    26305040
REFERENCE   2  (bases 1 to 863)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/yea.3098"; 
            journalName: "Yeast"; date: "2015-12"; volume: "32"; issue: "12"; 
            pages: "711-20"
COMMENT     Sequence Label: pT040
FEATURES             Location/Qualifiers
     source          1..863
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        33..301
                     /label=SNR52 promoter
                     /note="promoter for the S. cerevisiae small nucleolar RNA 
                     gene SNR52"
     terminator      383..402
                     /label=SUP4 terminator
                     /note="transcription terminator for the S. cerevisiae SUP4 
                     tRNA gene"
     primer_bind     477..493
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     477..493
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(539..555)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(540..556)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     promoter        complement(588..606)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(627..643)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(627..643)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(640..662)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    651..667
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(675..681,682..699,700..705))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    720..741
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 tatagggcga attggagctc caccgcggac aatctttgaa aagataatgt atgattatgc
       61 tttcactcat atttatacag aaacttgatg ttttctttcg agtatataca aggtgattac
      121 atgtacgttt gaagtacaac tctagatttt gtagtgccct cttgggctag cggtaaaggt
      181 gcgcattttt tcacacccta caatgttctg ttcaaaagat tttggtcaaa cgctgtagaa
      241 gtgaaagttg gtgcgcatgt ttcggcgttc gaaacttctc cgcagtgaaa gataaatgat
      301 cagaatcgat gcatttaaat agcaagttaa aataaggcta gtccgttatc aacttgaaaa
      361 agtggcaccg agtcggtggt gctttttttg ttttttatgt cttcgagtca tgtaattagt
      421 tataagcatg tgagctaaga cactgtaatt gccaatctaa acgataccac ggccgctcta
      481 gaactagtgg atcccccccg ctcgagcggg ggctgcagga attcgatatc aagcttatcg
      541 ataccgtcga cctcgatcga gggggggccc ggtaccagct tttgttccct ttagtgaggg
      601 ttaatttcga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
      661 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
      721 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
      781 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
      841 gggcgctctt ccgcttcctc gct
//
