LOCUS       sequence-121124-        1130 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  middle entry plasmid with dimerizable extracellular domain fused to 
            um200 mutant Danio Flt4 cytoplasmic domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1130)
  AUTHORS   Shin M, Male I, Beane TJ, Villefranc JA, Kok FO, Zhu LJ, Lawson ND
  TITLE     Vegfc acts through ERK to induce sprouting and differentiation of 
            trunk lymphatic progenitors.
  JOURNAL   Development. 2016 Oct 15;143(20):3785-3795. Epub 2016 Sep 12.
  PUBMED    27621059
REFERENCE   2  (bases 1 to 1130)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Development."; date: "2016-10-15"; volume: "143(20)"; pages: 
            "3785-3795. Epub 2016 Sep 12"
COMMENT     Sequence Label: pME-Fv2E-flt4um200-ns
FEATURES             Location/Qualifiers
     source          1..1130
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    9..108
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             114..155
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSKPKDPSQR"
     CDS             162..482
                     /codon_start=1
                     /product="F36V mutant of FK506-binding protein FKBP12"
                     /label=DmrB
                     /note="binds synthetic ligands that do not bind wild-type 
                     FKBP"
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKVDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     CDS             489..809
                     /codon_start=1
                     /product="F36V mutant of FK506-binding protein FKBP12"
                     /label=DmrB
                     /note="binds synthetic ligands that do not bind wild-type 
                     FKBP"
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKVDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
ORIGIN
        1 tcgggcccca aataatgatt ttattttgac tgatagtgac ctgttcgttg caacaaattg
       61 atgagcaatg cttttttata atgccaactt tgtacaaaaa agcaggctgg accatgggga
      121 gtagcaagag caagcctaag gaccccagcc agcgctctag aggcgtccaa gtcgaaacca
      181 ttagtcccgg cgatggcaga acatttccta aaaggggaca aacatgtgtc gtccattata
      241 caggcatgtt ggaggacggc aaaaaggtgg acagtagtag agatcgcaat aaacctttca
      301 aattcatgtt gggaaaacaa gaagtcatta ggggatggga ggagggcgtg gctcaaatgt
      361 ccgtcggcca acgcgctaag ctcaccatca gccccgacta cgcatacggc gctaccggac
      421 atcccggaat tattccccct cacgctacct tggtgtttga cgtcgaactg ttgaagctcg
      481 agactagagg agtgcaggtg gagactatct ccccaggaga cgggcgcacc ttccccaagc
      541 gcggccagac ctgcgtggtg cactacaccg ggatgcttga agatggaaag aaagttgatt
      601 cctcccggga cagaaacaag ccctttaagt ttatgctagg caagcaggag gtgatccgag
      661 gctgggaaga aggggttgcc cagatgagtg tgggtcagag agccaaactg actatatctc
      721 cagattatgc ctatggtgcc actgggcacc caggcatcat cccaccacat gccactctcg
      781 tcttcgatgt ggagcttcta aaactggaaa ctagagtgaa tgtcaagcga gtgaacccag
      841 ctgatatcaa gacaggctat ctgtccatca tcatggaccc gggggaagtg ccgctggagg
      901 agcagtgtga atacctgccg tacgactcca gccagtggga gatttccaga gacagactgc
      961 gcctcgggaa ggtgctcggc catggagctt tcgggaaggt gattgaagcc tcaatctttg
     1021 gtcatgacaa gaaatcctct gcaaacacag tggctgtcaa gatgttgaaa gagggagcaa
     1081 cagccagcga gcacaaagcc ctgatgtcag agctgaagat cctcattcat
//
