LOCUS       sequence-121139-         845 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  OR2-OR1-Pr2 promoter expressing deGFP-MG aptamer fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 845)
  AUTHORS   Siegal-Gaskins D, Tuza ZA, Kim J, Noireaux V, Murray RM
  TITLE     Gene circuit performance characterization and resource usage in a 
            cell-free ""breadboard"".
  JOURNAL   ACS Synth Biol. 2014 Jun 20;3(6):416-25. doi: 10.1021/sb400203p. 
            Epub 2014 Apr 7.
  PUBMED    24670245
REFERENCE   2  (bases 1 to 845)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb400203p";
            journalName: "ACS Synth Biol"; date: "2014-06-20- 20"; volume: "3"; 
            issue: "6"; pages: "416-25"
COMMENT     Sequence Label: Pr2-deGFP-MGapt
FEATURES             Location/Qualifiers
     source          1..845
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             157..179
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(216..237)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(477..496)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     824..845
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ttgctgggtg ggctaacgat atccgcctga tgcgtgaacg tgacggacgt aaccaccgcg
       61 acatgtgtgt gctgttccgc tgggcatgct gagctaacac cgtgcgtgtt gacaatttta
      121 cctctggcgg tgctaatggt tgcagctagc aataattttg tttaacttta agaaggagat
      181 ataccatgga gcttttcact ggcgttgttc ccatcctggt cgagctggac ggcgacgtaa
      241 acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga
      301 ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca
      361 ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact
      421 tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg
      481 acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca
      541 tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt
      601 acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg
      661 tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc
      721 agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca
      781 cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt
      841 tcgtg
//
