LOCUS       sequence-121194-         833 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway destination vector for cell-free expression with N-term 
            8xHis tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 833)
  AUTHORS   Gagoski D, Mureev S, Giles N, Johnston W, Dahmer-Heath M, Skalamera 
            D, Gonda TJ, Alexandrov K
  TITLE     Gateway-compatible vectors for high-throughput protein expression in
            pro- and eukaryotic cell-free systems.
  JOURNAL   J Biotechnol. 2015 Feb 10;195:1-7. doi: 
            10.1016/j.jbiotec.2014.12.006. Epub 2014 Dec 19.
  PUBMED    25529348
REFERENCE   2  (bases 1 to 833)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J 
            Biotechnol."; date: "2015-02-10"; volume: "195:1-7. doi"; pages: " 
            10.1016/j.jbiotec.2014.12.006. Epub 2014 Dec 19"
COMMENT     Sequence Label: pCellFree_G01
FEATURES             Location/Qualifiers
     source          1..833
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    168..292
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        439..541
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(608..627)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 ggtaccacgc gtacccaaca cacggacaca tgcacgaaca cgcatgcaga gagatcatac
       61 atcatctata gagaagtaca cgtaaaagta agactcgtcg tctgccagct acaaccgaga
      121 aggcgacgtg aaaggaacaa gaaaggagga ggagggcggc cgcttacacc actttgtaca
      181 agaaagctga acgagaaacg taaaatgata taaatatcaa tatattaaat tagattttgc
      241 ataaaaaaca gactacataa tactgtaaaa cacaacatat ccagtcacta tgaagcttgc
      301 taagttggca gcatcacccg acgcactttg cgccgaataa atacctgtga cggaagatca
      361 cttcgcagaa taaataaatc ctggtgtccc tgttgatacc gggaagccct gggccaactt
      421 ttggcgaaaa tgagacgttg atcggcacgc aagaggttcc aactttcacc ataatgaaat
      481 aagatcacta ccgggcgtat tttttgagtt atcgagattt tcaggagcta aggaagctaa
      541 aatggagaaa aaaatcactg gatataccac cgttgatata tcccaatggc atcgtaaaga
      601 acattttgag gcatttcagt cagttgctca atgtacctat aaccagaccg ttcagctgga
      661 tattacggcc tttttaaaga ccgtaaagaa aaataagcac aagttttatc cggcctttat
      721 tcacattctt gcccgcctga tgaatgctca tccggaattc cgtatggcaa tgaaagacgg
      781 tgagctggtg atatgggata gtgttcaccc ttgttacacc gttttccatg agc
//
