LOCUS       sequence-121235-         782 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  cloning of sgRNAs for expression in plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 782)
  AUTHORS   Ma X, Zhang Q, Zhu Q, Liu W, Chen Y, Qiu R, Wang B, Yang Z, Li H, 
            Lin Y, Xie Y, Shen R, Chen S, Wang Z, Chen Y, Guo J, Chen L, Zhao X,
            Dong Z, Liu YG
  TITLE     A robust CRISPR/Cas9 system for convenient, high-efficiency 
            multiplex genome editing in monocot and dicot plants.
  JOURNAL   Mol Plant. 2015 Apr 24. pii: S1674-2052(15)00204-X. doi: 
            10.1016/j.molp.2015.04.007.
  PUBMED    25917172
REFERENCE   2  (bases 1 to 782)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Plant. 
            2015 Apr 24. pii: S1674-2052(15)00204-X. doi: 
            10.1016/j.molp.2015.04.007."
COMMENT     Sequence Label: pYLsgRNA-OsU6b
FEATURES             Location/Qualifiers
     source          1..782
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    79..100
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(115..120,121..138,139..145)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    153..169
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     158..180
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     177..193
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     177..193
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     misc_RNA        243..318
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     primer_bind     complement(752..769)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(752..768)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg
       61 cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca
      121 ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg
      181 aaacagctat gaccatgatt acgaattcga gctcggtacc cggggatcca gcgtgggtct
      241 cggttttaga gctagaaata gcaagttaaa ataaggctag tccgttatca acttgaaaaa
      301 gtggcaccga gtcggtgctt tttttcaaga gcttggagtg gatggaattt tcctccgttt
      361 tacctgtgga atcggcagca aaggatgcaa gaacgaacta agccggacaa aaaaaaaagg
      421 agcacatata caaaccggtt ttattcatga atggtcacga tggatgatgg ggctcagact
      481 tgagctacga ggccgcaggc gagagaagcc tagtgtgctc tctgcttgtt tgggccgtaa
      541 cggaggatac ggcccacgag cgtgtactac cgcgcgggat gccgctgggc gctgcggggg
      601 ccgttggatg gggatcggtg ggtcgcggga gcgttgaggg gagacaggtt tagtaccacc
      661 tcgcctaccg aacaatgaag aacccacctt ataaccccgc gcgctgccgc ttgtgttgag
      721 agacctctga agataacata ctaagcttgg cactggccgt cgttttacaa cgtcgtgact
      781 gg
//
