LOCUS       sequence-121251-         485 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AAV vector to express dsRed sh RNA (scramble).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 485)
  AUTHORS   Zhang Z, Ferretti V, Guntan I, Moro A, Steinberg EA, Ye Z, Zecharia 
            AY, Yu X, Vyssotski AL, Brickley SG, Yustos R, Pillidge ZE, Harding 
            EC, Wisden W, Franks NP
  TITLE     Neuronal ensembles sufficient for recovery sleep and the sedative 
            actions of alpha2 adrenergic agonists.
  JOURNAL   Nat Neurosci. 2015 Apr;18(4):553-61. doi: 10.1038/nn.3957. Epub 2015
            Feb 23.
  PUBMED    25706476
REFERENCE   2  (bases 1 to 485)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nn.3957"; 
            journalName: "Nat Neurosci"; date: "2015-04"; volume: "18"; issue: 
            "4"; pages: "553-61"
COMMENT     Sequence Label: AAV-actin prom-dsRed-scramble shRNA
FEATURES             Location/Qualifiers
     source          1..485
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    94..127
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(271..289)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     complement(321..341)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
ORIGIN
        1 acttcgtata aagtatccta tacgaagtta tatcaaaata ggaagaccaa tgcttcacca
       61 tcgacccgaa ttgccaagca tcaccatcga cccataactt cgtataatgt atgctatacg
      121 aagttatgaa ttcctgcagg atggactcct ccgaggacgt catcaaggag ttcatgcgct
      181 tcaaggtgcg catggagggc tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg
      241 agggccgccc ctacgagggc acccagaccg ccaagctgaa ggtgaccaag ggcggccccc
      301 tgcccttcgc ctgggacatc ctgtcccccc agttccagta cggctccaag gtgtacgtga
      361 agcaccccgc cgacatcccc gactacaaga agctgtcctt ccccgagggc ttcaagtggg
      421 agcgcgtgat gaacttcgag gacggcggcg tggtgaccgt gacccaggac tcctccttac
      481 aggac
//
