LOCUS       sequence-121252-        1091 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pETDuet-1 Containing FadD Q339G.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1091)
  AUTHORS   Ford TJ, Way JC
  TITLE     Enhancement of E. coli acyl-CoA synthetase FadD activity on medium 
            chain fatty acids.
  JOURNAL   PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717/peerj.1040. eCollection 
            2015.
  PUBMED    26157619
REFERENCE   2  (bases 1 to 1091)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PeerJ."; 
            date: "2015-06-30"; volume: "3:e1040. doi"; pages: " 
            10.7717/peerj.1040. eCollection 2015"
COMMENT     Sequence Label: PAS525 (TJF092)
FEATURES             Location/Qualifiers
     source          1..1091
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(49..66)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ttcgtcgatt agcattatgc ggccgcagct tggatcctca ttcantcagt gatgatgatg
       61 gtgatgagaa gaacctcagg ctttattgtc cactttgccg cgcgcttcgt cacgtaattc
      121 tcgtcgcaaa atttttccga cgttagattt cggtaactca tcacgaaact ccaccagctt
      181 cggtactttg tagcccgtga gctgacggcg gcaaaaggtc accagtgact cttcggtaag
      241 cgatggatct tttttcacta cgaagatttt caccgcttca ccactggagc cggaaggtac
      301 gccaacagcc gcgacttcct gtacgccagg atgctgcatg acgacatctt caatctcgtt
      361 gggatagacg ttaaaaccgg aaaccagaat catgtctttt ttacgatcga caatgcgcag
      421 gaacccttct tcatccatca ccgcgatgtc gccggtgtgt aaccagccat ttttgatgat
      481 ctcatctgta gcatccggac gctgccagta acccagcatc acctgcggtc ctttgacaca
      541 aagctcaccc ggttgacccg gtggtacttc attatcatca tcatccacca gtttggcttc
      601 cgtcgacggc accggcaaac cgatgctacc actatgataa tcaatatcat atgggttaac
      661 gctgaccagc ggcgcacact cggtaaggcc atagccttcc agcagatact gtcctgtcag
      721 tttcacccaa cgctctgcca ccacgccctg cactggcatc cctccgcctg cggaaagatg
      781 cagactggag aaatccagct gctggaactc tttattgttc agcaacgcat tgaacaaggt
      841 gttaacgccc gtgatagcgg taaacggata tttcgctaac tcttttacca accctggaat
      901 atcgcgcggg ttagtgataa gcaggttctg cccacccagt tcgataaaca gcaggcagtt
      961 aatggtcagg gcaaaaatgt gatacagcgg cagcgccgtc accaccagct ctttgcccgg
     1021 atgcaacagc ggaccatagg tcgcgttaac ctgttccagg ttcgccagca tattgcggtg
     1081 agtcagcatc g
//
