LOCUS       sequence-121279-         803 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of ER localized oxBFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 803)
  AUTHORS   Costantini LM, Baloban M, Markwardt ML, Rizzo M, Guo F, Verkhusha 
            VV, Snapp EL
  TITLE     A palette of fluorescent proteins optimized for diverse cellular 
            environments.
  JOURNAL   Nat Commun. 2015 Jul 9;6:7670. doi: 10.1038/ncomms8670.
  PUBMED    26158227
REFERENCE   2  (bases 1 to 803)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2015-07-9"; volume: "6:7670. doi"; pages: " 
            10.1038/ncomms8670"
COMMENT     Sequence Label: ERoxBFP
FEATURES             Location/Qualifiers
     source          1..803
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      10..19
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(29..121)
                     /codon_start=1
                     /product="signal sequence from bovine prolactin"
                     /label=prolactin signal sequence
                     /translation="MDSKGSSQKGSRLLLLLVVSNLLLCQGVVST"
     5'UTR           complement(130..172)
                     /label=Xenopus globin 5'-UTR
                     /note="translational enhancer from the 5'-UTR of the major 
                     beta-globin gene of Xenopus laevis"
     promoter        complement(200..403)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(229..249)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        404..707
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 ccttgctcac catggtggcg accggtccgg tggagaccac accctggcac aagagtagat
       61 ttgacaccac cagcagcagg agcaggcggg accctttctg cgacgaacct ttgctgtcca
      121 tggtatctgc caaagttgag cgtttattct gagcttctgc aaaaagaaca agctagcgga
      181 tctgacggtt cactaaacca gctctgctta tatagacctc ccaccgtaca cgcctaccgc
      241 ccatttgcgt caatggggcg gagttgttac gacattttgg aaagtcccgt tgattttggt
      301 gccaaaacaa actcccattg acgtcaatgg ggtggagact tggaaatccc cgtgagtcaa
      361 accgctatcc acgcccattg atgtactgcc aaaaccgcat caccatggta atagcgatga
      421 ctaatacgta gatgtactgc caagtaggaa agtcccataa ggtcatgtac tgggcataat
      481 gccaggcggg ccatttaccg tcattgacgt caataggggg cgtacttggc atatgataca
      541 cttgatgtac tgccaagtgg gcagtttacc gtaaatactc cacccattga cgtcaatgga
      601 aagtccctat tggcgttact atgggaacat acgtcattat tgacgtcaat gggcgggggt
      661 cgttgggcgg tcagccaggc gggccattta ccgtaagtta tgtaacgcgg aactccatat
      721 atgggctatg aactaatgac cccgtaattg attactatta ataactaatg catggcggta
      781 atacggttat ccacagaatc agg
//
