LOCUS       sequence-121313-         924 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of hSara with C-term Myc tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 924)
  AUTHORS   Kerr SC, Kahn RA
  TITLE     Tool box: Plasmids for the expression or knockdown of human ARF 
            Family GTPases (ARF/ARL/SAR) and their co-expression in bacteria 
            with N-myristoyltransferases.
  JOURNAL   Cell Logist. 2015 Sep 21;5(3):e1090523. doi: 
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep.
  PUBMED    27057421
REFERENCE   2  (bases 1 to 924)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Logist."; date: "2015-09-21"; volume: "5(3):e1090523. doi"; pages: "
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep"
COMMENT     Sequence Label: pDSHA-SARA-Myc
FEATURES             Location/Qualifiers
     source          1..924
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(20..49)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     protein_bind    63..87
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(700..724)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(835..853)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 attaaggtac cgggccctac agatcctctt cagagatgag tttctgctcc atctcgaatc
       61 gaaccacttt gtacaagaaa gctgggtcat caatgtactg tgccatccag cggaagcctt
      121 ctccgtaacc ttgtcttttg agcacactac acatgaaaac ttctaagggt cgggcattca
      181 gttctttcag agatatactc ccctttcctg ttgtctgacc atataaacca aacatctctc
      241 gcaacctctc ttcactgatg gcttcaggtc tgtcgatctt attcccaaga atcagtatag
      301 gcacattagc aatggtttca tctgtcatta gtgaatcaag ttcttctttt gactctaaca
      361 gcctttcgtg gtctgcacaa tccaccagaa atacaatgcc attgatagca ggaaggtagt
      421 ttttccacac tcttcgagct tgaacatgtc cacccagatc aaaagttgta aacgtcatgc
      481 cagcaatggt cagttcttcg gaagtgggat gtaatgttgg gacatgttgt ccaagtctgt
      541 catcttttag catgtgtagc aatgttgttt ttcctgcatt atccaatcca agaaatacca
      601 gtttaccagt tttcttatat aatcctaaaa actgtagcac actgctgaaa ccactgtaaa
      661 tccaatcaaa tatgaaggac atggttctat ctccttctaa gcctgctttt ttgtacaaac
      721 ttgtttgatt cgagtgtcga cggtatcgat aagcttgata tcgaattcct gcagcccggg
      781 ggatccgccc gggctagagc ggccgccacc gcggtggagc tccagctttt gttcccttta
      841 gtgagggtta atttcgagct tggcgtaatc gctagcggaa tctgacggtt cactaaacca
      901 gctctgctta tatagacctc ccac
//
