LOCUS       sequence-121315-        6521 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Rhamnose-inducible Int12 expression vector with rpiL*C51 RBS, pNBU2 
            backbone, AmpR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6521)
  AUTHORS   Mimee M, Tucker AC, Voigt CA, Lu TK
  TITLE     Programming a Human Commensal Bacterium, Bacteroides 
            thetaiotaomicron, to Sense and Respond to Stimuli in the Murine Gut 
            Microbiota.
  JOURNAL   Cell Syst. 2015 Jul 29;1(1):62-71. doi: 10.1016/j.cels.2015.06.001.
  PUBMED    26918244
REFERENCE   2  (bases 1 to 6521)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cels.2015.06"; journalName: "Cell Syst"; date: 
            "2015-07-29- 29"; volume: "1"; issue: "1"; pages: "62-71"
COMMENT     Sequence Label: pAT937
FEATURES             Location/Qualifiers
     source          1..6521
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(3022..3090,3091..3882)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3240..3259)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     oriT            4013..4122
                     /note="incP origin of transfer"
     rep_origin      4293..4681
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
ORIGIN
        1 ccttgttgct catattgtga tgataatttc tacaaatata gtcattggta actatctatg
       61 aaactgtttg atacttttat agttgattaa acttgttcat ggcatttgcc ttaatatcat
      121 ccgctatgtc aatgtagggt ttcatagctt tgtagtcgct gtgtcccgtc catttcatga
      181 ccacctgtgc cgggattccg agagccagcg cattgcagat gaatgtcctt cttcctgcat
      241 gggtactgag caaagcgtat ttgggtgtga cttcatcaat acgttcattt cccttgtagt
      301 aggtttcccg tacaggctcg ttgatttctg ccagttcgcc cagctctttc aggtaatcgt
      361 tcatcttctg gttgctgatg acgggcagag ccatgtaatt ctcgaaatgg atgtccttgt
      421 atttgtccag tatggctttg ctgtatttgt tcagttcaat cgtcaggctg tcggcagtct
      481 tgactgtggt tatttcgatg tggtcggact tcacatcgct tcttttcaga ttgcgaacat
      541 ccgaataccg caaactcgta aagcagcaga acaggaaaac atcacgcaca cgttccaggt
      601 attgcttatc cttgggtatc tggtagtctt tcagcttgtt cagttcatcc caagtcagga
      661 agattacttt tttcgaggtg gttttcagtt tcggtttgaa cgtatcgtat gcaatgttct
      721 gatgatgtcc tttcttgaag ctccagcgca ggaaccattt gaggaatccc atttgcttgc
      781 cgatggtgct gtttctcata tccttggtgt cacgcaggaa gttgacgtat tcgttcaatc
      841 caaactcgtt gaaatagttg aacgttgcat cctccttgaa ctctttgagg tggttcctca
      901 ctgctgcaaa tttttcatag gtggatgccg tccagttatt ctggttaccg cactctttta
      961 caaactcatc gaacacctcc caaaagctga caggggcttc ttccggctgt tcttcgctgg
     1021 tgtctttcat tctcatgttg aaagcttcct tcaactgttg ggtcgttggc atgacctcct
     1081 gcacctcaaa ttccttgaaa atattctgga tttcggcata gtatttcagc aagtccgtat
     1141 tgatttcggc tgcactttgc tttagcttgt tggtacatcc gctctttacc cgctgcttat
     1201 ctgcatccca tttggctacg tcaatccggt agcccgttgt aaactcgatg cgttggctgg
     1261 caaagatgac acgcatacgg atgggtacgt tctctacgat tggcacaccg ttctttttcc
     1321 ggctctccaa tgcaaaaatg atgttgcgct tgatattcat aattgggtgc gtttgaaatt
     1381 ctacacccaa atatacaccc aattattgag atagcaaaag acatttagaa acatttactt
     1441 ttactctata ttgtaattta cacttgatta tcagtcgttt gcagtcttat gatattctgt
     1501 gaaagtataa gttcgagagc ctgtctctcc gcaaaaaacg ctgaaaatca gcagattgca
     1561 aaacaaacac cctgttttac acccaagaat gtaaagtcgg ctgtttttgt tttatttaag
     1621 ataatacaac cactacataa taaaagagta gcgatattaa aagaatccga tgagaaaaga
     1681 ctaatattta tctatccatt cagtttgatt tttcaggact ttacatcgtc ctgaaagtat
     1741 ttgttggtac cggtaccgag gacgcgtaaa catttacagt tgcatgtggc ctattgtttt
     1801 tagccgttaa atattttata actattaaat agcgatacaa attgttcgaa actaatattg
     1861 tttatatcat atattctcgc atgttttaaa gctttattaa attgattttt tgtaaacagt
     1921 ttttcgtact ctttgttaac ccatttcatt acaaaagttt catatttttt tctctcttta
     1981 aatgccattt ttgctggctt tctttttaat acaattaatg tgctatccac tttaggtttt
     2041 ggatggaaat aatacctagg aatttttgct aatatagaaa tatctacctc tgccattaac
     2101 agcaatgcta gtgatctgtt tgtatctaat aacattttag caaaaccata ttccactatt
     2161 aaataactta ttgtggctga actttcaaaa acaatttttc gaattatatt tgtgcttatg
     2221 ttgtaaggta tgctgccaaa tattttatat ggattgtggc taggaaatgt aaatttcagt
     2281 atatcatcat ttactatttg atagttagga taatttaaga gcttattacg agttacctca
     2341 cataatttag aatcaatttc tatcgccgtt acaaaattac atctctttac caatccagca
     2401 gtaaaatgac ctttccctgc acctatttca aagatgttat ctttttcatc taaacttatg
     2461 caattcatta ttttttctat gtgatatttt gaagtaataa aattttgact atcttttata
     2521 tttactttgt tcattataac ctctccttaa tttattgcat ctcttttcga atatttatgt
     2581 tttttgagaa aagaacgtac tcatggttca tcccgatatg cgtatcggtc tgtatatcag
     2641 caactttcta tgtgtttcaa ctacaatagt catctattct catctttctg agtccacccc
     2701 ctgcaaagcc cctctttacg acataaaaat tcggtcggaa aaggtatgca aaagatgttt
     2761 ctctctttaa gagaaactct tcgggatgca aaaatatgaa aataactcca attcaccaaa
     2821 ttatatagcg acttttttac aaaatgctaa aatttgttga tttccgtcaa gcaattgttg
     2881 agcaaaaatg tcttttacga taaaatgata cctcaatatc aactgtttag caaaacgata
     2941 tttctcttaa agagagaaac acctttttgt tcaccaatcc ccgactttta atcccgcggc
     3001 catgattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
     3061 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
     3121 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
     3181 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
     3241 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata
     3301 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
     3361 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
     3421 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
     3481 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
     3541 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact
     3601 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
     3661 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
     3721 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
     3781 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
     3841 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
     3901 tcataacgcg tcaattcgag ggggatcaat tccgtgatag gtgggctgcc cttcctggtt
     3961 ggcttggttt catcagccat ccgcttgccc tcatctgtta cgccggcggt agccggccag
     4021 cctcgcagag caggattccc gttgagcacc gccaggtgcg aataagggac agtgaagaag
     4081 gaacacccgc tcgcgggtgg gcctacttca cctatcctgc ccggctgacg ccgttggata
     4141 caccaaggaa agtctacacg aaccctttgg caaaatcctg tatatcgtgc gaaaaaggat
     4201 ggatataccg aaaaaatcgc tataatgacc ccgaagcagg gttatgcagc ggaaaacgga
     4261 attgatccgg ccacgatgcg tccggcgtag aggatctgaa gatcagcagt tcaacctgtt
     4321 gatagtacgt actaagctct catgtttcac gtactaagct ctcatgttta acgtactaag
     4381 ctctcatgtt taacgaacta aaccctcatg gctaacgtac taagctctca tggctaacgt
     4441 actaagctct catgtttcac gtactaagct ctcatgtttg aacaataaaa ttaatataaa
     4501 tcagcaactt aaatagcctc taaggtttta agttttataa gaaaaaaaag aatatataag
     4561 gcttttaaag cttttaaggt ttaacggttg tggacaacaa gccagggatg taacgcactg
     4621 agaagccctt agagcctctc aaagcaattt tgagtgacac aggaacactt aacggctgac
     4681 atgggaattc ccctccaccg cggtggtatt tcggagaaaa catgcataaa tcatgctttt
     4741 tttgcataaa aagtaaaatt tatactgatg taaggtttgg ctatgcagat ttgtgtcaaa
     4801 atgcacatcc tttctatcaa aatgcgtaag gaaaaggagg aaggaaccgc ctatctttgc
     4861 aatgtacgca ttttaaaata aaataaatta ttattttatt taaacgaata tgaaagtggc
     4921 catttatacc cgtgttagca gcgcagaaca ggcaaatgaa ggttatagca ttcacgagca
     4981 gaagaagaaa ctgatcagct attgcgaaat ccacgattgg aacgagtata aagtttttac
     5041 cgatgcaggt attagcggtg gtagcatgaa acgtccggca ctgcaaaaac tgatgaaaca
     5101 tctgagttca tttgatctgg tgctggtgta taaactggat cgtctgaccc gtaatgttcg
     5161 tgatctgctg gatatgctgg aagaatttga acagtataac gtgagcttta aaagcgccac
     5221 cgaagttttt gataccacca gtgcaattgg caaactgttt attaccatgg ttggtgcaat
     5281 ggcagaatgg gaacgtgaaa ccattcgtga acgtagcctg tttggtagcc gtgcagcagt
     5341 tcgtgaaggt aactatattc gtgaagcacc gttttgctat gataacattg aaggtaaact
     5401 gcacccgaac gaatatgcca aagttattga tctgattgtg agcatgttca aaaaaggcat
     5461 tagcgccaat gaaattgcac gtcgtctgaa tagcagcaaa gttcatgttc cgaacaaaaa
     5521 aagctggaat cgtaatagcc tgattcgtct gatgcgtagt ccggttctgc gtggtcatac
     5581 caaatatggt gatatgctga ttgaaaacac ccatgaaccg gtgctgagcg aacatgatta
     5641 taatgcaatt aacaacgcca tcagcagcaa aacccataaa agcaaagtta aacaccatgc
     5701 catttttcgt ggtgcactgg tttgtccgca gtgtaatcgt cgtctgcatc tgtatgcagg
     5761 caccgttaaa gatcgtaaag gctataaata cgatgtgcgt cgctataaat gtgaaacctg
     5821 cagcaaaaac aaagatgtga agaatgtgag cttcaacgaa agcgaagtgg aaaacaaatt
     5881 cgtcaatctg ctgaaaagct acgagctgaa caaatttcat atccgtaaag tggaaccggt
     5941 gaaaaaaatc gagtatgaca tcgataagat taacaaacag aaaattaact atacccgcag
     6001 ttggagcctg ggctatattg aagatgatga atatttcgag ctgatggaag aaatcaacgc
     6061 caccaaaaaa atgatcgaag aacagaccac cgagaataaa cagagcgtta gcaaagagca
     6121 gattcagagc attaacaact ttatcctgaa aggctgggaa gaactgacca tcaaagataa
     6181 agaggaactg attctgagca ccgtggataa aatcgaattt aacttcatcc cgaaagataa
     6241 aaaacataaa accaataccc tggatattaa caatattcac tttaaattct aatgaactgc
     6301 acttgctttg ataattaatg ataaacaatc taaaagcact ctaatcgtta tcggagtgct
     6361 tttagattac taatcaaatt gcttctacta attgcctatc ttccagtgat ggaacagcat
     6421 ttgtgcattg gctgcaacaa tcagccttac ttgtgcctgt tctatttccg aaccgaccgc
     6481 ttgtatgaat ccatcaaaat tcgttttctc tatgttggat t
//
