LOCUS       sequence-121344-         732 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cre-dependent expression of FlpO from CAV2 virus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 732)
  AUTHORS   Schwarz LA, Miyamichi K, Gao XJ, Beier KT, Weissbourd B, DeLoach KE,
            Ren J, Ibanes S, Malenka RC, Kremer EJ, Luo L
  TITLE     Viral-genetic tracing of the input-output organization of a central 
            noradrenaline circuit.
  JOURNAL   Nature. 2015 Aug 6;524(7563):88-92. doi: 10.1038/nature14600. Epub 
            2015 Jul 1.
  PUBMED    26131933
REFERENCE   2  (bases 1 to 732)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature14600"; journalName: "Nature"; date: "2015-08-6- 6"; 
            volume: "524"; issue: "7563"; pages: "88-92"
COMMENT     Sequence Label: pTCAV-FLEx(loxP)-FlpO
FEATURES             Location/Qualifiers
     source          1..732
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    56..89
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    160..193
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
ORIGIN
        1 accctcacta aagggaagcg gcctgtacag catgcatgat ctagtaatta agcttataac
       61 ttcgtataat gtatgctata cgaagttatg ggtcgatggt gagatctgga ctagagggtc
      121 gatggtgatg cttggatcaa aataggaaga atcccaatga taacttcgta taaagtatcc
      181 tatacgaagt tataggcgcg ccaccatgag ccagttcgac atcctgtgca agaccccccc
      241 caaggtgctg gtgcggcagt tcgtggagag attcgagagg cccagcggcg agaagatcgc
      301 cagctgtgcc gccgagctga cctacctgtg ctggatgatc acccacaacg gcaccgccat
      361 caagagggcc accttcatga gctacaacac catcatcagc aacagcctga gcttcgacat
      421 cgtgaacaag agcctgcagt tcaagtacaa gacccagaag gccaccatcc tggaggccag
      481 cctgaagaag ctgatccccg cctgggagtt caccatcatc ccttacaacg gccagaagca
      541 ccagagcgac atcaccgaca tcgtgtccag cctgcagctg cagttcgaga gcagcgagga
      601 ggccgacaag ggcaacagcc acagcaagaa gatgctgaag gccctgctgt ccgagggcga
      661 gagcatctgg gagatcaccg agaagatcct gaacagcttc gagtacacca gcaggttcac
      721 caagaccaag ac
//
