LOCUS       sequence-121368-        5517 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  NanoLuc expressed constiutively from PcepA, pNBU2 backbone, AmpR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5517)
  AUTHORS   Mimee M, Tucker AC, Voigt CA, Lu TK
  TITLE     Programming a Human Commensal Bacterium, Bacteroides 
            thetaiotaomicron, to Sense and Respond to Stimuli in the Murine Gut 
            Microbiota.
  JOURNAL   Cell Syst. 2015 Jul 29;1(1):62-71. doi: 10.1016/j.cels.2015.06.001.
  PUBMED    26918244
REFERENCE   2  (bases 1 to 5517)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cels.2015.06"; journalName: "Cell Syst"; date: 
            "2015-07-29- 29"; volume: "1"; issue: "1"; pages: "62-71"
COMMENT     Sequence Label: pMM580
FEATURES             Location/Qualifiers
     source          1..5517
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(3804..3872,3873..4664)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4022..4041)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     oriT            4795..4904
                     /note="incP origin of transfer"
     rep_origin      5075..5463
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
ORIGIN
        1 acctttgtgg accgtattac aaagaaccca atcatatatg gtttttactc tggaagattt
       61 tgttggcgat tggcgtcaga ccgcgggtta taatttggat caagtcctgg aacagggtgg
      121 cgtaagctct ctgttccaga acctgggtgt gagcgtgacg ccgattcagc gcatcgttct
      181 gtccggcgag aacggtctga aaattgatat tcatgtgatc atcccgtacg aaggcctgag
      241 cggtgaccaa atgggtcaaa tcgagaaaat ctttaaagtc gtctacccag ttgacgatca
      301 ccacttcaag gttatcttgc attacggtac gctggtgatt gatggtgtga ccccgaatat
      361 gattgactat ttcggccgtc cgtatgaagg cattgccgtt tttgacggta aaaagatcac
      421 cgtcaccggt accctgtgga atggcaataa gattattgac gagcgtctga ttaacccgga
      481 cggcagcctg ctgttccgcg tgaccatcaa cggtgtcacg ggttggcgtc tgtgcgagcg
      541 catcctggca taatgaactg cacttgcttt gataattaat gataaacaat ctaaaagcac
      601 tctaatcgtt atcggagtgc ttttagatta ctaatcaaat tgcttctact aattgcctat
      661 cttccagtga tggaacagca tttgtgcatt ggctgcaaca atcagcctta cttgtgcctg
      721 ttctatttcc gaaccgaccg cttgtatgaa tccatcaaaa ttcgttttct ctatgttgga
      781 ttccttgttg ctcatattgt gatgataatt tctacaaata tagtcattgg taactatcta
      841 tgaaactgtt tgatactttt atagttgatt aaacttgttc atggcatttg ccttaatatc
      901 atccgctatg tcaatgtagg gtttcatagc tttgtagtcg ctgtgtcccg tccatttcat
      961 gaccacctgt gccgggattc cgagagccag cgcattgcag atgaatgtcc ttcttcctgc
     1021 atgggtactg agcaaagcgt atttgggtgt gacttcatca atacgttcat ttcccttgta
     1081 gtaggtttcc cgtacaggct cgttgatttc tgccagttcg cccagctctt tcaggtaatc
     1141 gttcatcttc tggttgctga tgacgggcag agccatgtaa ttctcgaaat ggatgtcctt
     1201 gtatttgtcc agtatggctt tgctgtattt gttcagttca atcgtcaggc tgtcggcagt
     1261 cttgactgtg gttatttcga tgtggtcgga cttcacatcg cttcttttca gattgcgaac
     1321 atccgaatac cgcaaactcg taaagcagca gaacaggaaa acatcacgca cacgttccag
     1381 gtattgctta tccttgggta tctggtagtc tttcagcttg ttcagttcat cccaagtcag
     1441 gaagattact tttttcgagg tggttttcag tttcggtttg aacgtatcgt atgcaatgtt
     1501 ctgatgatgt cctttcttga agctccagcg caggaaccat ttgaggaatc ccatttgctt
     1561 gccgatggtg ctgtttctca tatccttggt gtcacgcagg aagttgacgt attcgttcaa
     1621 tccaaactcg ttgaaatagt tgaacgttgc atcctccttg aactctttga ggtggttcct
     1681 cactgctgca aatttttcat aggtggatgc cgtccagtta ttctggttac cgcactcttt
     1741 tacaaactca tcgaacacct cccaaaagct gacaggggct tcttccggct gttcttcgct
     1801 ggtgtctttc attctcatgt tgaaagcttc cttcaactgt tgggtcgttg gcatgacctc
     1861 ctgcacctca aattccttga aaatattctg gatttcggca tagtatttca gcaagtccgt
     1921 attgatttcg gctgcacttt gctttagctt gttggtacat ccgctcttta cccgctgctt
     1981 atctgcatcc catttggcta cgtcaatccg gtagcccgtt gtaaactcga tgcgttggct
     2041 ggcaaagatg acacgcatac ggatgggtac gttctctacg attggcacac cgttcttttt
     2101 ccggctctcc aatgcaaaaa tgatgttgcg cttgatattc ataattgggt gcgtttgaaa
     2161 ttctacaccc aaatatacac ccaattattg agatagcaaa agacatttag aaacatttac
     2221 ttttactcta tattgtaatt tacacttgat tatcagtcgt ttgcagtctt atgatattct
     2281 gtgaaagtat aagttcgaga gcctgtctct ccgcaaaaaa cgctgaaaat cagcagattg
     2341 caaaacaaac accctgtttt acacccaaga atgtaaagtc ggctgttttt gttttattta
     2401 agataataca accactacat aataaaagag tagcgatatt aaaagaatcc gatgagaaaa
     2461 gactaatatt tatctatcca ttcagtttga tttttcagga ctttacatcg tcctgaaagt
     2521 atttgttggt accggtaccg aggacgcgta aacatttaca gttgcatgtg gcctattgtt
     2581 tttagccgtt aaatatttta taactattaa atagcgatac aaattgttcg aaactaatat
     2641 tgtttatatc atatattctc gcatgtttta aagctttatt aaattgattt tttgtaaaca
     2701 gtttttcgta ctctttgtta acccatttca ttacaaaagt ttcatatttt tttctctctt
     2761 taaatgccat ttttgctggc tttcttttta atacaattaa tgtgctatcc actttaggtt
     2821 ttggatggaa ataataccta ggaatttttg ctaatataga aatatctacc tctgccatta
     2881 acagcaatgc tagtgatctg tttgtatcta ataacatttt agcaaaacca tattccacta
     2941 ttaaataact tattgtggct gaactttcaa aaacaatttt tcgaattata tttgtgctta
     3001 tgttgtaagg tatgctgcca aatattttat atggattgtg gctaggaaat gtaaatttca
     3061 gtatatcatc atttactatt tgatagttag gataatttaa gagcttatta cgagttacct
     3121 cacataattt agaatcaatt tctatcgccg ttacaaaatt acatctcttt accaatccag
     3181 cagtaaaatg acctttccct gcacctattt caaagatgtt atctttttca tctaaactta
     3241 tgcaattcat tattttttct atgtgatatt ttgaagtaat aaaattttga ctatctttta
     3301 tatttacttt gttcattata acctctcctt aatttattgc atctcttttc gaatatttat
     3361 gttttttgag aaaagaacgt actcatggtt catcccgata tgcgtatcgg tctgtatatc
     3421 agcaactttc tatgtgtttc aactacaata gtcatctatt ctcatctttc tgagtccacc
     3481 ccctgcaaag cccctcttta cgacataaaa attcggtcgg aaaaggtatg caaaagatgt
     3541 ttctctcttt aagagaaact cttcgggatg caaaaatatg aaaataactc caattcacca
     3601 aattatatag cgactttttt acaaaatgct aaaatttgtt gatttccgtc aagcaattgt
     3661 tgagcaaaaa tgtcttttac gataaaatga tacctcaata tcaactgttt agcaaaacga
     3721 tatttctctt aaagagagaa acaccttttt gttcaccaat ccccgacttt taatcccgcg
     3781 gccatgattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
     3841 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
     3901 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
     3961 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
     4021 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca
     4081 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
     4141 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
     4201 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
     4261 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
     4321 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
     4381 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
     4441 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
     4501 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
     4561 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
     4621 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
     4681 actcataacg cgtcaattcg agggggatca attccgtgat aggtgggctg cccttcctgg
     4741 ttggcttggt ttcatcagcc atccgcttgc cctcatctgt tacgccggcg gtagccggcc
     4801 agcctcgcag agcaggattc ccgttgagca ccgccaggtg cgaataaggg acagtgaaga
     4861 aggaacaccc gctcgcgggt gggcctactt cacctatcct gcccggctga cgccgttgga
     4921 tacaccaagg aaagtctaca cgaacccttt ggcaaaatcc tgtatatcgt gcgaaaaagg
     4981 atggatatac cgaaaaaatc gctataatga ccccgaagca gggttatgca gcggaaaacg
     5041 gaattgatcc ggccacgatg cgtccggcgt agaggatctg aagatcagca gttcaacctg
     5101 ttgatagtac gtactaagct ctcatgtttc acgtactaag ctctcatgtt taacgtacta
     5161 agctctcatg tttaacgaac taaaccctca tggctaacgt actaagctct catggctaac
     5221 gtactaagct ctcatgtttc acgtactaag ctctcatgtt tgaacaataa aattaatata
     5281 aatcagcaac ttaaatagcc tctaaggttt taagttttat aagaaaaaaa agaatatata
     5341 aggcttttaa agcttttaag gtttaacggt tgtggacaac aagccaggga tgtaacgcac
     5401 tgagaagccc ttagagcctc tcaaagcaat tttgagtgac acaggaacac ttaacggctg
     5461 acatgggaat tcccctccac cgcggtggca aatttgcgcg ccacaattat tattcat
//
