LOCUS       sequence-121394-       13898 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway binary vector designed for transgenic research with 
            Marchantia polymorpha as well as other plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13898)
  AUTHORS   Ishizaki K, Nishihama R, Ueda M, Inoue K, Ishida S, Nishimura Y, 
            Shikanai T, Kohchi T
  TITLE     Development of Gateway Binary Vector Series with Four Different 
            Selection Markers for the Liverwort Marchantia polymorpha.
  JOURNAL   PLoS One. 2015 Sep 25;10(9):e0138876. doi: 
            10.1371/journal.pone.0138876. eCollection 2015.
  PUBMED    26406247
REFERENCE   2  (bases 1 to 13898)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-09-25"; volume: "10(9):e0138876. doi"; pages: " 
            10.1371/journal.pone.0138876. eCollection 2015"
COMMENT     Sequence Label: pMpGWB303
FEATURES             Location/Qualifiers
     source          1..13898
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    54..78
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     266..288
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     280..297
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2057..2181
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(2218..2223,2224..2241,2242..2248)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             2302..2961
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(2368..2387)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             3303..3608
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     3487..3506
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(3649..3773)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      3805..4057
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(4091..4107)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4091..4107)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4104..4126)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4115..4131
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4139..4145,4146..4163,4164..4169))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4184..4205
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     polyA_signal    4290..4465
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        complement(6549..7225)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     complement(6592..6612)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     misc_feature    7420..7444
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             7965..8756
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      9002..9590
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9491..9510
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9744..9761
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    9776..9916
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(9842..9861)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     10002..10024
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      10260..10454
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(10520..11593)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(12022..12651)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttggcc cgggcaaatg agtcacacac attgttgaga gacatatcaa aataactcaa
      361 ttgtaaggta agttgataga tttcccagtt aaatgcatta ttttaacaat atttgtatct
      421 caaattttca aaatcatatc aaatttgcag atcttgatga tgattcacaa ctctgtcttc
      481 gatttttgat tgttaacggg tatcgatatt tgggtaactt aaaaagaatt tgctaaagat
      541 cttaaaaaac agattaaaaa aacctttcaa aatagctaat cctttaaatc cgtttcacaa
      601 tataatgctc attgatgata atcgaaaaga ttccatttta aagtgcatat aaattgctta
      661 attatctctt tctgtctcgt aatattttgt tctctcctat tggatcggac ccgcatcttc
      721 tcgatcagtt cactatacta atatttggtc aacttcaaga gagattagaa cttcgttctc
      781 caaagtgtaa aggccgacaa gcagaaaagg aaacttcaat cagaagcctt ctaggttttc
      841 ccgcgtccca aaaagcgaag ggaggacggg gaggacggtg aggccagaag aagaaagcaa
      901 cagtaaaatc tcatgtgatt gcgtgcatgt catggatcta tgggatgcta gtccaccgtt
      961 tggaagtatg tgcagaagga gaaaggcgcg ggaggccaaa agcgacttcg gccacctctc
     1021 tactttctag aaagtttgct cgcagcttgt aataagcgcg gaaaaggtac tcgaaaaacc
     1081 ggagcaatcg gtgtggtatc agcagtcggg ttcgctaaac ctacgtcttc ttattttgga
     1141 atgttaaatt gaaagggcgt gccggcccta agcacggacg atgtgacaga cggaggagtg
     1201 tgagcaattc ggatgacgat gctggccggc agtcacgaca aagagtccga aggggcatgg
     1261 acagggcaat atataaatgg aaaagaggct ttgaatattc ctaacccttc gtcgtcggcc
     1321 tcccaacttg ccctcgcatg ctgaattggc cgcctatctg cgaatggccg gccgccgacc
     1381 ctaagtcgca ataacttgaa cggagccgat gcaaagtcct tatcgaacgc tcgttattat
     1441 aaacggggca gacctaggta tttaattata tcaaaaccct ccgcaatttc gctaatcatt
     1501 ccctaatttc ttctactttg gcgtctagtg ctgtggtcgc gcttgctctc aaagctcgct
     1561 gggaggcagt ggagcgtctg gcttaaaatc gatcgccgca accctagagt tcctttcact
     1621 tctttctctc ttgcgtggag agcatcgaca tcgaagagct gcatcttgtc ttctggtatg
     1681 cgccttgtgc cctcgtctct gtacgtcgtt acgattgctc gatattgtga ttgtctagac
     1741 ctgaaacccg actggagctt ttacgtggtg tcgattgggg cctcgagcga gtgatttttt
     1801 aggttcgttt tagccaagta catcccactt tggtcagtcc tgttatctcg gtaaggtgtt
     1861 cagttcggtc tctggtgcgg aggtgcctcg taagtttcct gcatgataga tttgtctatc
     1921 cttgacttga agaggagtcg aaggtcagcg aggttggctg tgtgctatgg acgtcggtgc
     1981 tccttggttc cgtgcttcta tgggtgttgc ctcaacagta ttgatgtgcc tgcagaaagg
     2041 ttgactagag ttatcaacaa gtttgtacaa aaaagctgaa cgagaaacgt aaaatgatat
     2101 aaatatcaat atattaaatt agattttgca taaaaaacag actacataat actgtaaaac
     2161 acaacatatc cagtcactat ggcggcccct cgaggggccg cattaggcac cccaggcttt
     2221 acactttatg cttccggctc gtataatgtg tggattttga gttaggatcc ggcgagattt
     2281 tcaggagcta aggaagctaa aatggagaaa aaaatcactg gatataccac cgttgatata
     2341 tcccaatggc atcgtaaaga acattttgag gcatttcagt cagttgctca atgtacctat
     2401 aaccagaccg ttcagctgga tattacggcc tttttaaaga ccgtaaagaa aaataagcac
     2461 aagttttatc cggcctttat tcacattctt gcccgcctga tgaatgctca tccggaattc
     2521 cgtatggcaa tgaaagacgg tgagctggtg atatgggata gtgttcaccc ttgttacacc
     2581 gttttccatg agcaaactga aacgttttca tcgctctgga gtgaatacca cgacgatttc
     2641 cggcagtttc tacacatata ttcgcaagat gtggcgtgtt acggtgaaaa cctggcctat
     2701 ttccctaaag ggtttattga gaatatgttt ttcgtctcag ccaatccctg ggtgagtttc
     2761 accagttttg atttaaacgt ggccaatatg gacaacttct tcgcccccgt tttcaccatg
     2821 ggcaaatatt atacgcaagg cgacaaggtg ctgatgccgc tggcgattca ggttcatcat
     2881 gccgtctgtg atggcttcca tgtcggcaga atgcttaatg aattacaaca gtactgcgat
     2941 gagtggcagg gcggggcgta aagatctgga tccggcttac taaaagccag ataacagtat
     3001 gcgtatttgc gcgctgattt ttgcggtata agaatatata ctgatatgta tacccgaagt
     3061 atgtcaaaaa gaggtgtgct atgaagcagc gtattacagt gacagttgac agcgacagct
     3121 atcagttgct caaggcatat atgatgtcaa tatctccggt ctggtaagca caaccatgca
     3181 gaatgaagcc cgtcgtctgc gtgccgaacg ctggaaagcg gaaaatcagg aagggatggc
     3241 tgaggtcgcc cggtttattg aaatgaacgg ctcttttgct gacgagaaca gggactggtg
     3301 aaatgcagtt taaggtttac acctataaaa gagagagccg ttatcgtctg tttgtggatg
     3361 tacagagtga tattattgac acgcccgggc gacggatggt gatccccctg gccagtgcac
     3421 gtctgctgtc agataaagtc tcccgtgaac tttacccggt ggtgcatatc ggggatgaaa
     3481 gctggcgcat gatgaccacc gatatggcca gtgtgccggt ctccgttatc ggggaagaag
     3541 tggctgatct cagccaccgc gaaaatgaca tcaaaaacgc cattaacctg atgttctggg
     3601 gaatataaat gtcaggctcc cttatacaca gccagtctgc aggtcgacca tagtgactgg
     3661 atatgttgtg ttttacagta ttatgtagtc tgttttttat gcaaaatcta atttaatata
     3721 ttgatattta tatcatttta cgtttctcgt tcagctttct tgtacaaagt ggttgataac
     3781 agcgcttaga gctcgaattt ccccgatcgt tcaaacattt ggcaataaag tttcttaaga
     3841 ttgaatcctg ttgccggtct tgcgatgatt atcatataat ttctgttgaa ttacgttaag
     3901 catgtaataa ttaacatgta atgcatgacg ttatttatga gatgggtttt tatgattaga
     3961 gtcccgcaat tatacattta atacgcgata gaaaacaaaa tatagcgcgc aaactaggat
     4021 aaattatcgc gcgcggtgtc atctatgtta ctagatcggg aattggttcc ggaaccaatt
     4081 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
     4141 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
     4201 cattaggctg aattaggcgc gcctatttct gaattcaaca cattgcggac gtttttaatg
     4261 tactgaatta acgccgaatt aattcggggg atctggattt tagtactgga ttttggtttt
     4321 aggaattaga aattttattg atagaagtat tttacaaata caaatacata ctaagggttt
     4381 cttatatgct caacacatga gcgaaaccct ataggaaccc taaattccct tatctgggaa
     4441 ctactcacac attattatgg agaaactcga agttagtata tagtccttcc atcaccctct
     4501 gtgataatct ccttaaaact tgctccccca ggaatcatag gcagaacgtg ctcctggtgg
     4561 ggaactatca catccagtaa ataaggaccg ggagaatcta gcatagtttg aattgctgct
     4621 cttagttcat ccttcttcac cacacgcgca tttggcacct tgaaaccgtc tgcaactgta
     4681 cagaagtcgg ggtagatctg ctcctcgtga tctggatcac ctaagtaagt atgtgctctg
     4741 ttggccttgt agaatctatc ctccaattgt acgaccattc ctagatgttg gttgtttaag
     4801 atcatgatct tcacgggtaa cttctccaca aaaattgtag ccaactcctg tacgttcatc
     4861 acaaagctac catcgccatc gatgtcgatc acaggaatat ctggttgagt tgcggcagcc
     4921 cccaaagcag aaggaagtcc gaatcccatg gctccaagac ctccagatgt caaccaacgc
     4981 cgcggttcgt tgtacttgaa ccactgggct gcccacattt gatgttggcc aacaccagta
     5041 gatatgatgg catttccacc agtgagttcg tacagcgtct gtatggcaga ttgaggagca
     5101 atggcatcct cgaatacggg aaacttcatt ggccatttct gcttttgttc ttctagttct
     5161 gccacccagt tggagaagtc gaaagtcctc ttttgactct tcaagagggc attgatttca
     5221 cttagcgcag gtttcacatc agcacacatg gacacatggg gctgtttgtt ctttccaatt
     5281 tcagcagggt caatatcgat atgaacgatg ctggacctgc tagcaaaagt ctccagttta
     5341 ccagtcactc gatcgtcgaa acgcactcca aatgccaaca acagatctgc ttggtccacg
     5401 gcgtagttgg cgtaaacagt tccatgcatt ccaagcattt tcaaacttaa tggatcagac
     5461 tcgggatacg ctcccaaacc cattaaagtc gatgtgatag gaattccggt aagctcgaca
     5521 aattccctca actcttcgct ggcgtgattg caacctcctc cagaatacac tacaggcttc
     5581 ttagaactag aaatgagtct tataatctgc tcgattaacg ttggttccgg tggccgaggc
     5641 agtcgttgta agtagccctt cagttgcatt ggctgagacc aatctggaat agccatttgc
     5701 tgttgcacgt ccttgggaat atctaccaag actgggcccg gcctacctga agctgccaaa
     5761 aagaaagcct ccctgattac gcgagggatg tcatcaatgt tcataacaag gtaattgtgc
     5821 ttggtgatcg atctcgtaac ctccacaatt ggagtttctt gaaaagcatc ggtaccaatc
     5881 atcctactgg atacttgacc tgtaatggcc actagtggca cactgtccaa gagggcatca
     5941 gcgagcccgg taacaagatt ggtcgctcca ggaccggatg tggctataca aacccctact
     6001 cgtccagacg atttagcata accttcagca gcgaaaatct ccccctgctc gtggcgacag
     6061 agaatgttgc ggatgctgct ggaacgtgta agagcctggt gaatctccat agacgctccg
     6121 ccgggataag cgaacaccgt tctaatccct tctcgctcaa gagcctccac caaaatgtcg
     6181 gctcctttgc ggggctcgtc atccttgtag cgagactgaa agtcggacgg ggttggtgca
     6241 acgagtgact tcccgctgct aagccgtggt ccactcacat cgactgcaga ctggatcttc
     6301 tcgctaacag cttgggcttt cacttccagt gaacgctcag tcttccgaga ggtgttgtat
     6361 gcatgtcccc tcagagcggc tcccgagaag aaaccactcc tcacctttag ctctttagct
     6421 cccttgcaac aagaccttgc cgatggctgc accttctcca ggctcgctat gctcaaaggc
     6481 acagctgcgc atccactaat tgacgccatg ctcgagagag atagatttgt agagagagac
     6541 tggtgatttc agcgtgtcct ctccaaatga aatgaacttc cttatataga ggaagggtct
     6601 tgcgaaggat agtgggattg tgcgtcatcc cttacgtcag tggagatatc acatcaatcc
     6661 acttgctttg aagacgtggt tggaacgtct tctttttcca cgatgctcct cgtgggtggg
     6721 ggtccatctt tgggaccact gtcggcagag gcatcttgaa cgatagcctt tcctttatcg
     6781 caattatggc atttgtaggt gccaccttcc ttttctactg tccttttgat gaagtgacag
     6841 atagctgggc aatggaatcc gaggaggttt cccgatatta ccctttgttg aaaagtctca
     6901 atagcccttt ggtcttctga gactgtatct ttgatattct tggagtagac gagagtgtcg
     6961 tgctccacca tgttcacatc aatccacttg ctttgaagac gtggttggaa cgtcttcttt
     7021 ttccacgatg ctcctcgtgg gtgggggtcc atctttggga ccactgtcgg cagaggcatc
     7081 ttgaacgata gcctttcctt tatcgcaatg atggcatttg taggtgccac cttccttttc
     7141 tactgtcctt ttgatgaagt gacagatagc tgggcaatgg aatccgagga ggtttcccga
     7201 tattaccctt tgttgaaaag tctcaatagc cctttggtct tctgagactg tatctttgat
     7261 attcttggag tagacgagag tgtcgtgctc caccatgttg gcaagctgct ctagccaata
     7321 cgcaaaccga attcagaaat aaattcagcc taattcggcg ttaattcagt acattaaaaa
     7381 cgtccgcaat gtgttattaa gttgtctaag cgtcaatttg tttacaccac aatatatcct
     7441 gccaccagcc agccaacagc tccccgaccg gcagctcggc acaaaatcac cactcgatac
     7501 aggcagccca tcagtccggg acggcgtcag cgggagagcc gttgtaaggc ggcagacttt
     7561 gctcatgtta ccgatgctat tcggaagaac ggcaactaag ctgccgggtt tgaaacacgg
     7621 atgatctcgc ggagggtagc atgttgattg taacgatgac agagcgttgc tgcctgtgat
     7681 caattcgggc acgaacccag tggacataag cctgttcggt tcgtaagctg taatgcaagt
     7741 agcgtatgcg ctcacgcaac tggtccagaa ccttgaccga acgcagcggt ggtaacggcg
     7801 cagtggcggt tttcatggct tgttatgact gtttttttgg ggtacagtct atgcctcggg
     7861 catccaagca gcaagcgcgt tacgccgtgg gtcgatgttt gatgttatgg agcagcaacg
     7921 atgttacgca gcagggcagt cgccctaaaa caaagttaaa catcatgggg gaagcggtga
     7981 tcgccgaagt atcgactcaa ctatcagagg tagttggcgt catcgagcgc catctcgaac
     8041 cgacgttgct ggccgtacat ttgtacggct ccgcagtgga tggcggcctg aagccacaca
     8101 gtgatattga tttgctggtt acggtgaccg taaggcttga tgaaacaacg cggcgagctt
     8161 tgatcaacga ccttttggaa acttcggctt cccctggaga gagcgagatt ctccgcgctg
     8221 tagaagtcac cattgttgtg cacgacgaca tcattccgtg gcgttatcca gctaagcgcg
     8281 aactgcaatt tggagaatgg cagcgcaatg acattcttgc aggtatcttc gagccagcca
     8341 cgatcgacat tgatctggct atcttgctga caaaagcaag agaacatagc gttgccttgg
     8401 taggtccagc ggcggaggaa ctctttgatc cggttcctga acaggatcta tttgaggcgc
     8461 taaatgaaac cttaacgcta tggaactcgc cgcccgactg ggctggcgat gagcgaaatg
     8521 tagtgcttac gttgtcccgc atttggtaca gcgcagtaac cggcaaaatc gcgccgaagg
     8581 atgtcgctgc cgactgggca atggagcgcc tgccggccca gtatcagccc gtcatacttg
     8641 aagctagaca ggcttatctt ggacaagaag aagatcgctt ggcctcgcgc gcagatcagt
     8701 tggaagaatt tgtccactac gtgaaaggcg agatcaccaa ggtagtcggc aaataatgtc
     8761 tagctagaaa ttcgttcaag ccgacgccgc ttcgcggcgc ggcttaactc aagcgttaga
     8821 tgcactaagc acataattgc tcacagccaa actatcaggt caagtctgct tttattattt
     8881 ttaagcgtgc ataataagcc ctacacaaat tgggagatat atcatgcatg accaaaatcc
     8941 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     9001 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     9061 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     9121 tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact
     9181 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     9241 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     9301 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     9361 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     9421 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     9481 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     9541 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     9601 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     9661 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     9721 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga
     9781 tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatgg tgcactctca
     9841 gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga
     9901 ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg
     9961 tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca
    10021 gaggttttca ccgtcatcac cgaaacgcgc gaggcagggt gccttgatgt gggcgccggc
    10081 ggtcgagtgg cgacggcgcg gcttgtccgc gccctggtag attgcctggc cgtaggccag
    10141 ccatttttga gcggccagcg gccgcgatag gccgacgcga agcggcgggg cgtagggagc
    10201 gcagcgaccg aagggtaggc gctttttgca gctcttcggc tgtgcgctgg ccagacagtt
    10261 atgcacaggc caggcgggtt ttaagagttt taataagttt taaagagttt taggcggaaa
    10321 aatcgccttt tttctctttt atatcagtca cttacatgtg tgaccggttc ccaatgtacg
    10381 gctttgggtt cccaatgtac gggttccggt tcccaatgta cggctttggg ttcccaatgt
    10441 acgtgctatc cacaggaaag agaccttttc gacctttttc ccctgctagg gcaatttgcc
    10501 ctagcatctg ctccgtacat taggaaccgg cggatgcttc gccctcgatc aggttgcggt
    10561 agcgcatgac taggatcggg ccagcctgcc ccgcctcctc cttcaaatcg tactccggca
    10621 ggtcatttga cccgatcagc ttgcgcacgg tgaaacagaa cttcttgaac tctccggcgc
    10681 tgccactgcg ttcgtagatc gtcttgaaca accatctggc ttctgccttg cctgcggcgc
    10741 ggcgtgccag gcggtagaga aaacggccga tgccgggatc gatcaaaaag taatcggggt
    10801 gaaccgtcag cacgtccggg ttcttgcctt ctgtgatctc gcggtacatc caatcagcta
    10861 gctcgatctc gatgtactcc ggccgcccgg tttcgctctt tacgatcttg tagcggctaa
    10921 tcaaggcttc accctcggat accgtcacca ggcggccgtt cttggccttc ttcgtacgct
    10981 gcatggcaac gtgcgtggtg tttaaccgaa tgcaggtttc taccaggtcg tctttctgct
    11041 ttccgccatc ggctcgccgg cagaacttga gtacgtccgc aacgtgtgga cggaacacgc
    11101 ggccgggctt gtctcccttc ccttcccggt atcggttcat ggattcggtt agatgggaaa
    11161 ccgccatcag taccaggtcg taatcccaca cactggccat gccggccggc cctgcggaaa
    11221 cctctacgtg cccgtctgga agctcgtagc ggatcacctc gccagctcgt cggtcacgct
    11281 tcgacagacg gaaaacggcc acgtccatga tgctgcgact atcgcgggtg cccacgtcat
    11341 agagcatcgg aacgaaaaaa tctggttgct cgtcgccctt gggcggcttc ctaatcgacg
    11401 gcgcaccggc tgccggcggt tgccgggatt ctttgcggat tcgatcagcg gccgcttgcc
    11461 acgattcacc ggggcgtgct tctgcctcga tgcgttgccg ctgggcggcc tgcgcggcct
    11521 tcaacttctc caccaggtca tcacccagcg ccgcgccgat ttgtaccggg ccggatggtt
    11581 tgcgaccgct cacgccgatt cctcgggctt gggggttcca gtgccattgc agggccggca
    11641 gacaacccag ccgcttacgc ctggccaacc gcccgttcct ccacacatgg ggcattccac
    11701 ggcgtcggtg cctggttgtt cttgattttc catgccgcct cctttagccg ctaaaattca
    11761 tctactcatt tattcatttg ctcatttact ctggtagctg cgcgatgtat tcagatagca
    11821 gctcggtaat ggtcttgcct tggcgtaccg cgtacatctt cagcttggtg tgatcctccg
    11881 ccggcaactg aaagttgacc cgcttcatgg ctggcgtgtc tgccaggctg gccaacgttg
    11941 cagccttgct gctgcgtgcg ctcggacggc cggcacttag cgtgtttgtg cttttgctca
    12001 ttttctcttt acctcattaa ctcaaatgag ttttgattta atttcagcgg ccagcgcctg
    12061 gacctcgcgg gcagcgtcgc cctcgggttc tgattcaaga acggttgtgc cggcggcggc
    12121 agtgcctggg tagctcacgc gctgcgtgat acgggactca agaatgggca gctcgtaccc
    12181 ggccagcgcc tcggcaacct caccgccgat gcgcgtgcct ttgatcgccc gcgacacgac
    12241 aaaggccgct tgtagccttc catccgtgac ctcaatgcgc tgcttaacca gctccaccag
    12301 gtcggcggtg gcccatatgt cgtaagggct tggctgcacc ggaatcagca cgaagtcggc
    12361 tgccttgatc gcggacacag ccaagtccgc cgcctggggc gctccgtcga tcactacgaa
    12421 gtcgcgccgg ccgatggcct tcacgtcgcg gtcaatcgtc gggcggtcga tgccgacaac
    12481 ggttagcggt tgatcttccc gcacggccgc ccaatcgcgg gcactgccct ggggatcgga
    12541 atcgactaac agaacatcgg ccccggcgag ttgcagggcg cgggctagat gggttgcgat
    12601 ggtcgtcttg cctgacccgc ctttctggtt aagtacagcg ataaccttca tgcgttcccc
    12661 ttgcgtattt gtttatttac tcatcgcatc atatacgcag cgaccgcatg acgcaagctg
    12721 ttttactcaa atacacatca cctttttaga cggcggcgct cggtttcttc agcggccaag
    12781 ctggccggcc aggccgccag cttggcatca gacaaaccgg ccaggatttc atgcagccgc
    12841 acggttgaga cgtgcgcggg cggctcgaac acgtacccgg ccgcgatcat ctccgcctcg
    12901 atctcttcgg taatgaaaaa cggttcgtcc tggccgtcct ggtgcggttt catgcttgtt
    12961 cctcttggcg ttcattctcg gcggccgcca gggcgtcggc ctcggtcaat gcgtcctcac
    13021 ggaaggcacc gcgccgcctg gcctcggtgg gcgtcacttc ctcgctgcgc tcaagtgcgc
    13081 ggtacagggt cgagcgatgc acgccaagca gtgcagccgc ctctttcacg gtgcggcctt
    13141 cctggtcgat cagctcgcgg gcgtgcgcga tctgtgccgg ggtgagggta gggcgggggc
    13201 caaacttcac gcctcgggcc ttggcggcct cgcgcccgct ccgggtgcgg tcgatgatta
    13261 gggaacgctc gaactcggca atgccggcga acacggtcaa caccatgcgg ccggccggcg
    13321 tggtggtgtc ggcccacggc tctgccaggc tacgcaggcc cgcgccggcc tcctggatgc
    13381 gctcggcaat gtccagtagg tcgcgggtgc tgcgggccag gcggtctagc ctggtcactg
    13441 tcacaacgtc gccagggcgt aggtggtcaa gcatcctggc cagctccggg cggtcgcgcc
    13501 tggtgccggt gatcttctcg gaaaacagct tggtgcagcc ggccgcgtgc agttcggccc
    13561 gttggttggt caagtcctgg tcgtcggtgc tgacgcgggc atagcccagc aggccagcgg
    13621 cggcgctctt gttcatggcg taatgtctcc ggttctagtc gcaagtattc tactttatgc
    13681 gactaaaaca cgcgacaaga aaacgccagg aaaagggcag ggcggcagcc tgtcgcgtaa
    13741 cttaggactt gtgcgacatg tcgttttcag aagacggctg cactgaacgt cagaagccga
    13801 ctgcactata gcagcggagg ggttggatca aagtactttg atcccgaggg gaaccctgtg
    13861 gttggcatgc acatacaaat ggacgaacgg ataaacct
//
