LOCUS       sequence-121412-        7650 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  activating MTOR mutation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 7650)
  AUTHORS   Grabiner BC, Nardi V, Birsoy K, Possemato R, Shen K, Sinha S, Jordan
            A, Beck AH, Sabatini DM
  TITLE     A diverse array of cancer-associated MTOR mutations are 
            hyperactivating and can predict rapamycin sensitivity.
  JOURNAL   Cancer Discov. 2014 May;4(5):554-63. doi: 
            10.1158/2159-8290.CD-13-0929. Epub 2014 Mar 14.
  PUBMED    24631838
REFERENCE   2  (bases 1 to 7650)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-13-0929"; journalName: "Cancer Discov"; date: 
            "2014-05"; volume: "4"; issue: "5"; pages: "554-63"
COMMENT     Sequence Label: pcDNA3-FLAG-MTOR-L1460P
FEATURES             Location/Qualifiers
     source          1..7650
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      2852..2861
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6061..6339
                     /codon_start=1
                     /product="FKBP-rapamycin binding domain of human FRAP"
                     /label=FRB
                     /translation="ILWHEMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTL
                     KETSFNQAYGRDLMEAQEWCRKYMKSGNVKDLTQAWDLYYHVFRRISK"
     CDS             6073..6345
                     /codon_start=1
                     /product="FKBP-rapamycin binding domain of human FRAP"
                     /label=FRB
                     /translation="EMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTLKETS
                     FNQAYGRDLMEAQEWCRKYMKSGNVKDLTQAWDLYYHVFRRISKQL"
ORIGIN
        1 atgcttggaa ccggacctgc cgccgccacc accgctgcca ccacatctag caatgtgagc
       61 gtcctgcagc agtttgccag tggcctaaag agccggaatg aggaaaccag ggccaaagcc
      121 gccaaggagc tccagcacta tgtcaccatg gaactccgag agatgagtca agaggagtct
      181 actcgcttct atgaccaact gaaccatcac atttttgaat tggtttccag ctcagatgcc
      241 aatgagagga aaggtggcat cttggccata gctagcctca taggagtgga aggtgggaat
      301 gccacccgaa ttggcagatt tgccaactat cttcggaacc tcctcccctc caatgaccca
      361 gttgtcatgg aaatggcatc caaggccatt ggccgtcttg ccatggcagg ggacactttt
      421 accgctgagt acgtggaatt tgaggtgaag cgagccctgg aatggctggg tgctgaccgc
      481 aatgagggcc ggagacatgc agctgtcctg gttctccgtg agctggccat cagcgtccct
      541 accttcttct tccagcaagt gcaacccttc tttgacaaca tttttgtggc cgtgtgggac
      601 cccaaacagg ccatccgtga gggagctgta gccgcccttc gtgcctgtct gattctcaca
      661 acccagcgtg agccgaagga gatgcagaag cctcagtggt acaggcacac atttgaagaa
      721 gcagagaagg gatttgatga gaccttggcc aaagagaagg gcatgaatcg ggatgatcgg
      781 atccatggag ccttgttgat ccttaacgag ctggtccgaa tcagcagcat ggagggagag
      841 cgtctgagag aagaaatgga agaaatcaca cagcagcagc tggtacacga caagtactgc
      901 aaagatctca tgggcttcgg aacaaaacct cgtcacatta cccccttcac cagtttccag
      961 gctgtacagc cccagcagtc aaatgccttg gtggggctgc tggggtacag ctctcaccaa
     1021 ggcctcatgg gatttgggac ctcccccagt ccagctaagt ccaccctggt ggagagccgg
     1081 tgttgcagag acttgatgga ggagaaattt gatcaggtgt gccagtgggt gctgaaatgc
     1141 aggaatagca agaactcgct gatccaaatg acaatcctta atttgttgcc ccgcttggct
     1201 gcattccgac cttctgcctt cacagatacc cagtatctcc aagataccat gaaccatgtc
     1261 ctaagctgtg tcaagaagga gaaggaacgt acagcggcct tccaagccct ggggctactt
     1321 tctgtggctg tgaggtctga gtttaaggtc tatttgcctc gcgtgctgga catcatccga
     1381 gcggccctgc ccccaaagga cttcgcccat aagaggcaga aggcaatgca ggtggacgcc
     1441 acagtcttca cttgcatcag catgctggct cgagcaatgg ggccaggcat ccagcaggat
     1501 atcaaggagc tgctggagcc catgctggca gtgggactaa gccctgccct cactgcagtg
     1561 ctctacgacc tgagccgtca gattccacag ctaaagaagg acattcaaga tgggctactg
     1621 aaaatgctgt ccctggtcct tatgcacaaa ccccttcgcc acccaggcat gcccaagggc
     1681 ctggcccatc agctggcctc tcctggcctc acgaccctcc ctgaggccag cgatgtgggc
     1741 agcatcactc ttgccctccg aacgcttggc agctttgaat ttgaaggcca ctctctgacc
     1801 caatttgttc gccactgtgc ggatcatttc ctgaacagtg agcacaagga gatccgcatg
     1861 gaggctgccc gcacctgctc ccgcctgctc acaccctcca tccacctcat cagtggccat
     1921 gctcatgtgg ttagccagac cgcagtgcaa gtggtggcag atgtgcttag caaactgctc
     1981 gtagttggga taacagatcc tgaccctgac attcgctact gtgtcttggc gtccctggac
     2041 gagcgctttg atgcacacct ggcccaggcg gagaacttgc aggccttgtt tgtggctctg
     2101 aatgaccagg tgtttgagat ccgggagctg gccatctgca ctgtgggccg actcagtagc
     2161 atgaaccctg cctttgtcat gcctttcctg cgcaagatgc tcatccagat tttgacagag
     2221 ttggagcaca gtgggattgg aagaatcaaa gagcagagtg cccgcatgct ggggcacctg
     2281 gtctccaatg ccccccgact catccgcccc tacatggagc ctattctgaa ggcattaatt
     2341 ttgaaactga aagatccaga ccctgatcca aacccaggtg tgatcaataa tgtcctggca
     2401 acaataggag aattggcaca ggttagtggc ctggaaatga ggaaatgggt tgatgaactt
     2461 tttattatca tcatggacat gctccaggat tcctctttgt tggccaaaag gcaggtggct
     2521 ctgtggaccc tgggacagtt ggtggccagc actggctatg tagtagagcc ctacaggaag
     2581 taccctactt tgcttgaggt gctactgaat tttctgaaga ctgagcagaa ccagggtaca
     2641 cgcagagagg ccatccgtgt gttagggctt ttaggggctt tggatcctta caagcacaaa
     2701 gtgaacattg gcatgataga ccagtcccgg gatgcctctg ctgtcagcct gtcagaatcc
     2761 aagtcaagtc aggattcctc tgactatagc actagtgaaa tgctggtcaa catgggaaac
     2821 ttgcctctgg atgagttcta cccagctgtg tccatggtgg ccctgatgcg gatcttccga
     2881 gaccagtcac tctctcatca tcacaccatg gttgtccagg ccatcacctt catcttcaag
     2941 tccctgggac tcaaatgtgt gcagttcctg ccccaggtca tgcccacgtt ccttaatgtc
     3001 attcgagtct gtgatggggc catccgggaa tttttgttcc agcagctggg aatgttggtg
     3061 tcctttgtga agagccacat cagaccttat atggatgaaa tagtcaccct catgagagaa
     3121 ttctgggtca tgaacacctc aattcagagc acgatcattc ttctcattga gcaaattgtg
     3181 gtagctcttg ggggtgaatt taagctctac ctgccccagc tgatcccaca catgctgcgt
     3241 gtcttcatgc atgacaacag cccaggccgc attgtctcta tcaagttact ggctgcaatc
     3301 cagctgtttg gcgccaacct ggatgactac ctgcatttac tgctgcctcc tattgttaag
     3361 ttgtttgatg cccctgaagc tccactgcca tctcgaaagg cagcgctaga gactgtggac
     3421 cgcctgacgg agtccctgga tttcactgac tatgcctccc ggatcattca ccctattgtt
     3481 cgaacactgg accagagccc agaactgcgc tccacagcca tggacacgct gtcttcactt
     3541 gtttttcagc tggggaagaa gtaccaaatt ttcattccaa tggtgaataa agttctggtg
     3601 cgacaccgaa tcaatcatca gcgctatgat gtgctcatct gcagaattgt caagggatac
     3661 acacttgctg atgaagagga ggatcctttg atttaccagc atcggatgct taggagtggc
     3721 caaggggatg cattggctag tggaccagtg gaaacaggac ccatgaagaa actgcacgtc
     3781 agcaccatca acctccaaaa ggcctggggc gctgccagga gggtctccaa agatgactgg
     3841 ctggaatggc tgagacggct gagcctggag ctgctgaagg actcatcatc gccctccctg
     3901 cgctcctgct gggccctggc acaggcctac aacccgatgg ccagggatct cttcaatgct
     3961 gcatttgtgt cctgctggtc tgaactgaat gaagatcaac aggatgagct catcagaagc
     4021 atcgagttgg ccctcacctc acaagacatc gctgaagtca cacagaccct cttaaacttg
     4081 gctgaattca tggaacacag tgacaagggc cccctgccac tgagagatga caatggcatt
     4141 gttctgctgg gtgagagagc tgccaagtgc cgagcatatg ccaaagcact acactacaaa
     4201 gaactggagt tccagaaagg ccccacccct gccattctag aatctctcat cagcattaat
     4261 aataagctac agcagccgga ggcagcggcc ggagtgttag aatatgccat gaaacacttt
     4321 ggagagctgg agatccaggc tacctggtat gagaaactgc acgagtggga ggatgcccct
     4381 gtggcctatg acaagaaaat ggacaccaac aaggacgacc cagagctgat gctgggccgc
     4441 atgcgctgcc tcgaggcctt gggggaatgg ggtcaactcc accagcagtg ctgtgaaaag
     4501 tggaccctgg ttaatgatga gacccaagcc aagatggccc ggatggctgc tgcagctgca
     4561 tggggtttag gtcagtggga cagcatggaa gaatacacct gtatgatccc tcgggacacc
     4621 catgatgggg cattttatag agctgtgctg gcactgcatc aggacctctt ctccttggca
     4681 caacagtgca ttgacaaggc cagggacctg ctggatgctg aattaactgc aatggcagga
     4741 gagagttaca gtcgggcata tggggccatg gtttcttgcc acatgctgtc cgagctggag
     4801 gaggttatcc agtacaaact tgtccccgag cgacgagaga tcatccgcca gatctggtgg
     4861 gagagactgc agggctgcca gcgtatcgta gaggactggc agaaaatcct tatggtgcgg
     4921 tcccttgtgg tcagccctca tgaagacatg agaacctggc tcaagtatgc aagcctgtgc
     4981 ggcaagagtg gcaggctggc tcttgctcat aaaactttag tgttgctcct gggagttgat
     5041 ccgtctcggc aacttgacca tcctctgcca acagttcacc ctcaggtgac ctatgcctac
     5101 atgaaaaaca tgtggaagag tgcccgcaag atcgatgcct tccagcacat gcagcatttt
     5161 gtccagacca tgcagcaaca ggcccagcat gccatcgcta ctgaggacca gcagcataag
     5221 caggaactgc acaagctcat ggcccgatgc ttcctgaaac ttggagagtg gcagctgaat
     5281 ctacagggca tcaatgagag cacaatcccc aaagtgctgc agtactacag cgccgccaca
     5341 gagcacgacc gcagctggta caaggcctgg catgcgtggg cagtgatgaa cttcgaagct
     5401 gtgctacact acaaacatca gaaccaagcc cgcgatgaga agaagaaact gcgtcatgcc
     5461 agcggggcca acatcaccaa cgccaccact gccgccacca cggccgccac tgccaccacc
     5521 actgccagca ccgagggcag caacagtgag agcgaggccg agagcaccga gaacagcccc
     5581 accccatcgc cgctgcagaa gaaggtcact gaggatctgt ccaaaaccct cctgatgtac
     5641 acggtgcctg ccgtccaggg cttcttccgt tccatctcct tgtcacgagg caacaacctc
     5701 caggatacac tcagagttct caccttatgg tttgattatg gtcactggcc agatgtcaat
     5761 gaggccttag tggagggggt gaaagccatc cagattgata cctggctaca ggttatacct
     5821 cagctcattg caagaattga tacgcccaga cccttggtgg gacgtctcat tcaccagctt
     5881 ctcacagaca ttggtcggta ccacccccag gccctcatct acccactgac agtggcttct
     5941 aagtctacca cgacagcccg gcacaatgca gccaacaaga ttctgaagaa catgtgtgag
     6001 cacagcaaca ccctggtcca gcaggccatg atggtgagcg aggagctgat ccgagtggcc
     6061 atcctctggc atgagatgtg gcatgaaggc ctggaagagg catctcgttt gtactttggg
     6121 gaaaggaacg tgaaaggcat gtttgaggtg ctggagccct tgcatgctat gatggaacgg
     6181 ggcccccaga ctctgaagga aacatccttt aatcaggcct atggtcgaga tttaatggag
     6241 gcccaagagt ggtgcaggaa gtacatgaaa tcagggaatg tcaaggacct cacccaagcc
     6301 tgggacctct attatcatgt gttccgacga atctcaaagc agctgcctca gctcacatcc
     6361 ttagagctgc aatatgtttc cccaaaactt ctgatgtgcc gggaccttga attggctgtg
     6421 ccaggaacat atgaccccaa ccagccaatc attcgcattc agtccatagc accgtctttg
     6481 caagtcatca catccaagca gaggccccgg aaattgacac ttatgggcag caacggacat
     6541 gagtttgttt tccttctaaa aggccatgaa gatctgcgcc aggatgagcg tgtgatgcag
     6601 ctcttcggcc tggttaacac ccttctggcc aatgacccaa catctcttcg gaaaaacctc
     6661 agcatccaga gatacgctgt catcccttta tcgaccaact cgggcctcat tggctgggtt
     6721 ccccactgtg acacactgca cgccctcatc cgggactaca gggagaagaa gaagatcctt
     6781 ctcaacatcg agcatcgcat catgttgcgg atggctccgg actatgacca cttgactctg
     6841 atgcagaagg tggaggtgtt tgagcatgcc gtcaataata cagctgggga cgacctggcc
     6901 aagctgctgt ggctgaaaag ccccagctcc gaggtgtggt ttgaccgaag aaccaattat
     6961 acccgttctt tagcggtcat gtcaatggtt gggtatattt taggcctggg agatagacac
     7021 ccatccaacc tgatgctgga ccgtctgagt gggaagatcc tgcacattga ctttggggac
     7081 tgctttgagg ttgctatgac ccgagagaag tttccagaga agattccatt tagactaaca
     7141 agaatgttga ccaatgctat ggaggttaca ggcctggatg gcaactacag aatcacatgc
     7201 cacacagtga tggaggtgct gcgagagcac aaggacagtg tcatggccgt gctggaagcc
     7261 tttgtctatg accccttgct gaactggagg ctgatggaca caaataccaa aggcaacaag
     7321 cgatcccgaa cgaggacgga ttcctactct gctggccagt cagtcgaaat tttggacggt
     7381 gtggaacttg gagagccagc ccataagaaa acggggacca cagtgccaga atctattcat
     7441 tctttcattg gagacggttt ggtgaaacca gaggccctaa ataagaaagc tatccagatt
     7501 attaacaggg ttcgagataa gctcactggt cgggacttct ctcatgatga cactttggat
     7561 gttccaacgc aagttgagct gctcatcaaa caagcgacat cccatgaaaa cctctgccag
     7621 tgctatattg gctggtgccc tttctggtaa
//
