LOCUS       sequence-121468-         781 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  sgRNA for LSL.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 781)
  AUTHORS   Li Y, Park A, Mou H, Colpan C, Bizhanova A, Akama-Garren E, Joshi N,
            Hendrickson EA, Feldser D, Yin H, Anderson DG, Jacks T, Weng Z, Xue 
            W
  TITLE     A versatile reporter system for CRISPR-mediated chromosomal 
            rearrangements.
  JOURNAL   Genome Biol. 2015 May 28;16(1):111.
  PUBMED    26018130
REFERENCE   2  (bases 1 to 781)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome 
            Biol."; date: "2015-05-28"; volume: "16(1)"; pages: "111"
COMMENT     Sequence Label: pX330.LoxP
FEATURES             Location/Qualifiers
     source          1..781
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        100..348
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     100..120
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     271..290
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        370..445
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
       61 tacggttcct ggccttttgc tggccttttg ctcacatgtg agggcctatt tcccatgatt
      121 ccttcatatt tgcatatacg atacaaggct gttagagaga taattggaat taatttgact
      181 gtaaacacaa agatattagt acaaaatacg tgacgtagaa agtaataatt tcttgggtag
      241 tttgcagttt taaaattatg ttttaaaatg gactatcata tgcttaccgt aacttgaaag
      301 tatttcgatt tcttggcttt atatatcttg tggaaaggac gaaacaccgc gtatagcata
      361 cattatacgg ttttagagct agaaatagca agttaaaata aggctagtcc gttatcaact
      421 tgaaaaagtg gcaccgagtc ggtgcttttt tgttttagag ctagaaatag caagttaaaa
      481 taaggctagt ccgtttttag cgcgtgcgcc aattctgcag acaaatggct ctagaggtac
      541 ccgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat
      601 tgacgtcaat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac
      661 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg
      721 acgtcaatga cggtaaatgg cccgcctggc attgtgccca gtacatgacc ttatgggact
      781 t
//
