LOCUS       sequence-121498-        9287 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MCS-containing binary vector designed for transgenic research with 
            Marchantia polymorpha as well as other plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9287)
  AUTHORS   Ishizaki K, Nishihama R, Ueda M, Inoue K, Ishida S, Nishimura Y, 
            Shikanai T, Kohchi T
  TITLE     Development of Gateway Binary Vector Series with Four Different 
            Selection Markers for the Liverwort Marchantia polymorpha.
  JOURNAL   PLoS One. 2015 Sep 25;10(9):e0138876. doi: 
            10.1371/journal.pone.0138876. eCollection 2015.
  PUBMED    26406247
REFERENCE   2  (bases 1 to 9287)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-09-25"; volume: "10(9):e0138876. doi"; pages: " 
            10.1371/journal.pone.0138876. eCollection 2015"
COMMENT     Sequence Label: pMpGWB400
FEATURES             Location/Qualifiers
     source          1..9287
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    54..78
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     266..288
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     280..297
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      362..614
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(648..664)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(648..664)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(661..683)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    672..688
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(696..702,703..720,721..726))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    741..762
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     polyA_signal    847..1021
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(1078..1875)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLL
                     SSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEH
                     QGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDI
                     ALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1189..1208)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     1799..1818
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(1938..2614)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     complement(1981..2001)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     misc_feature    2809..2833
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             3354..4145
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      4391..4979
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4880..4899
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5133..5150
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    5165..5305
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(5231..5250)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5391..5413
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      5649..5843
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(5909..6982)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(7411..8040)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgcat gcctgcaggt cgactctaga ggatccccgg gtaccgagct cgaatttccc
      361 cgatcgttca aacatttggc aataaagttt cttaagattg aatcctgttg ccggtcttgc
      421 gatgattatc atataatttc tgttgaatta cgttaagcat gtaataatta acatgtaatg
      481 catgacgtta tttatgagat gggtttttat gattagagtc ccgcaattat acatttaata
      541 cgcgatagaa aacaaaatat agcgcgcaaa ctaggataaa ttatcgcgcg cggtgtcatc
      601 tatgttacta gatcgggaat tggttccgga accaattcgt aatcatggtc atagctgttt
      661 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
      721 tgtaaagcct ggggtgccta atgagtgagc taactcacat taggctgaat taggcgcgcc
      781 tatttctgaa ttcaacacat tgcggacgtt tttaatgtac tgaattaacg ccgaattaat
      841 tcgggggatc tggattttag tactggattt tggttttagg aattagaaat tttattgata
      901 gaagtatttt acaaatacaa atacatacta agggtttctt atatgctcaa cacatgagcg
      961 aaaccctata ggaaccctaa ttcccttatc tgggaactac tcacacatta ttatggagaa
     1021 actcgagctt gtcgatcgac tctagctaga ggatcgatcc gaaccccaga gtcccgctca
     1081 gaagaactcg tcaagaaggc gatagaaggc gatgcgctgc gaatcgggag cggcgatacc
     1141 gtaaagcacg aggaagcggt cagcccattc gccgccaagc tcttcagcaa tatcacgggt
     1201 agccaacgct atgtcctgat agcggtccgc cacacccagc cggccacagt cgatgaatcc
     1261 agaaaagcgg ccattttcca ccatgatatt cggcaagcag gcatcgccat gtgtcacgac
     1321 gagatcctcg ccgtcgggca tgcgcgcctt gagcctggcg aacagttcgg ctggcgcgag
     1381 cccctgatgc tcttcgtcca gatcatcctg atcgacaaga ccggcttcca tccgagtacg
     1441 tgctcgctcg atgcgatgtt tcgcttggtg gtcgaatggg caggtagccg gatcaagcgt
     1501 atgcagccgc cgcattgcat cagccatgat ggatactttc tcggcaggag caaggtgaga
     1561 tgacaggaga tcctgccccg gcacttcgcc caatagcagc cagtcccttc ccgcttcagt
     1621 gacaacgtcg agcacagctg cgcaaggaac gcccgtcgtg gccagccacg atagccgcgc
     1681 tgcctcgtcc tggagttcat tcagggcacc ggacaggtcg gtcttgacaa aaagaaccgg
     1741 gcgcccctgc gctgacagcc ggaacacggc ggcatcagag cagccgattg tctgttgtgc
     1801 ccagtcatag ccgaatagcc tctccaccca agcggccgga gaacctgcgt gcaatccatc
     1861 ttgttcaatc cccatggtcg atcgacagat ctgcgaaagc tcgagagaga tagatttgta
     1921 gagagagact ggtgatttca gcgtgtcctc tccaaatgaa atgaacttcc ttatatagag
     1981 gaagggtctt gcgaaggata gtgggattgt gcgtcatccc ttacgtcagt ggagatatca
     2041 catcaatcca cttgctttga agacgtggtt ggaacgtctt ctttttccac gatgctcctc
     2101 gtgggtgggg gtccatcttt gggaccactg tcggcagagg catcttgaac gatagccttt
     2161 cctttatcgc aatgatggca tttgtaggtg ccaccttcct tttctactgt ccttttgatg
     2221 aagtgacaga tagctgggca atggaatccg aggaggtttc ccgatattac cctttgttga
     2281 aaagtctcaa tagccctttg gtcttctgag actgtatctt tgatattctt ggagtagacg
     2341 agagtgtcgt gctccaccat gttcacatca atccacttgc tttgaagacg tggttggaac
     2401 gtcttctttt tccacgatgc tcctcgtggg tgggggtcca tctttgggac cactgtcggc
     2461 agaggcatct tgaacgatag cctttccttt atcgcaatga tggcatttgt aggtgccacc
     2521 ttccttttct actgtccttt tgatgaagtg acagatagct gggcaatgga atccgaggag
     2581 gtttcccgat attacccttt gttgaaaagt ctcaatagcc ctttggtctt ctgagactgt
     2641 atctttgata ttcttggagt agacgagagt gtcgtgctcc accatgttgg caagctgctc
     2701 tagccaatac gcaaaccgaa ttcagaaata aattcagcct aattcggcgt taattcagta
     2761 cattaaaaac gtccgcaatg tgttattaag ttgtctaagc gtcaatttgt ttacaccaca
     2821 atatatcctg ccaccagcca gccaacagct ccccgaccgg cagctcggca caaaatcacc
     2881 actcgataca ggcagcccat cagtccggga cggcgtcagc gggagagccg ttgtaaggcg
     2941 gcagactttg ctcatgttac cgatgctatt cggaagaacg gcaactaagc tgccgggttt
     3001 gaaacacgga tgatctcgcg gagggtagca tgttgattgt aacgatgaca gagcgttgct
     3061 gcctgtgatc aattcgggca cgaacccagt ggacataagc ctgttcggtt cgtaagctgt
     3121 aatgcaagta gcgtatgcgc tcacgcaact ggtccagaac cttgaccgaa cgcagcggtg
     3181 gtaacggcgc agtggcggtt ttcatggctt gttatgactg tttttttggg gtacagtcta
     3241 tgcctcgggc atccaagcag caagcgcgtt acgccgtggg tcgatgtttg atgttatgga
     3301 gcagcaacga tgttacgcag cagggcagtc gccctaaaac aaagttaaac atcatggggg
     3361 aagcggtgat cgccgaagta tcgactcaac tatcagaggt agttggcgtc atcgagcgcc
     3421 atctcgaacc gacgttgctg gccgtacatt tgtacggctc cgcagtggat ggcggcctga
     3481 agccacacag tgatattgat ttgctggtta cggtgaccgt aaggcttgat gaaacaacgc
     3541 ggcgagcttt gatcaacgac cttttggaaa cttcggcttc ccctggagag agcgagattc
     3601 tccgcgctgt agaagtcacc attgttgtgc acgacgacat cattccgtgg cgttatccag
     3661 ctaagcgcga actgcaattt ggagaatggc agcgcaatga cattcttgca ggtatcttcg
     3721 agccagccac gatcgacatt gatctggcta tcttgctgac aaaagcaaga gaacatagcg
     3781 ttgccttggt aggtccagcg gcggaggaac tctttgatcc ggttcctgaa caggatctat
     3841 ttgaggcgct aaatgaaacc ttaacgctat ggaactcgcc gcccgactgg gctggcgatg
     3901 agcgaaatgt agtgcttacg ttgtcccgca tttggtacag cgcagtaacc ggcaaaatcg
     3961 cgccgaagga tgtcgctgcc gactgggcaa tggagcgcct gccggcccag tatcagcccg
     4021 tcatacttga agctagacag gcttatcttg gacaagaaga agatcgcttg gcctcgcgcg
     4081 cagatcagtt ggaagaattt gtccactacg tgaaaggcga gatcaccaag gtagtcggca
     4141 aataatgtct agctagaaat tcgttcaagc cgacgccgct tcgcggcgcg gcttaactca
     4201 agcgttagat gcactaagca cataattgct cacagccaaa ctatcaggtc aagtctgctt
     4261 ttattatttt taagcgtgca taataagccc tacacaaatt gggagatata tcatgcatga
     4321 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     4381 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     4441 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     4501 taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag
     4561 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     4621 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     4681 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     4741 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     4801 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     4861 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     4921 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     4981 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     5041 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
     5101 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
     5161 agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatggt
     5221 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc
     5281 gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg
     5341 acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg
     5401 catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagggtg ccttgatgtg
     5461 ggcgccggcg gtcgagtggc gacggcgcgg cttgtccgcg ccctggtaga ttgcctggcc
     5521 gtaggccagc catttttgag cggccagcgg ccgcgatagg ccgacgcgaa gcggcggggc
     5581 gtagggagcg cagcgaccga agggtaggcg ctttttgcag ctcttcggct gtgcgctggc
     5641 cagacagtta tgcacaggcc aggcgggttt taagagtttt aataagtttt aaagagtttt
     5701 aggcggaaaa atcgcctttt ttctctttta tatcagtcac ttacatgtgt gaccggttcc
     5761 caatgtacgg ctttgggttc ccaatgtacg ggttccggtt cccaatgtac ggctttgggt
     5821 tcccaatgta cgtgctatcc acaggaaaga gaccttttcg acctttttcc cctgctaggg
     5881 caatttgccc tagcatctgc tccgtacatt aggaaccggc ggatgcttcg ccctcgatca
     5941 ggttgcggta gcgcatgact aggatcgggc cagcctgccc cgcctcctcc ttcaaatcgt
     6001 actccggcag gtcatttgac ccgatcagct tgcgcacggt gaaacagaac ttcttgaact
     6061 ctccggcgct gccactgcgt tcgtagatcg tcttgaacaa ccatctggct tctgccttgc
     6121 ctgcggcgcg gcgtgccagg cggtagagaa aacggccgat gccgggatcg atcaaaaagt
     6181 aatcggggtg aaccgtcagc acgtccgggt tcttgccttc tgtgatctcg cggtacatcc
     6241 aatcagctag ctcgatctcg atgtactccg gccgcccggt ttcgctcttt acgatcttgt
     6301 agcggctaat caaggcttca ccctcggata ccgtcaccag gcggccgttc ttggccttct
     6361 tcgtacgctg catggcaacg tgcgtggtgt ttaaccgaat gcaggtttct accaggtcgt
     6421 ctttctgctt tccgccatcg gctcgccggc agaacttgag tacgtccgca acgtgtggac
     6481 ggaacacgcg gccgggcttg tctcccttcc cttcccggta tcggttcatg gattcggtta
     6541 gatgggaaac cgccatcagt accaggtcgt aatcccacac actggccatg ccggccggcc
     6601 ctgcggaaac ctctacgtgc ccgtctggaa gctcgtagcg gatcacctcg ccagctcgtc
     6661 ggtcacgctt cgacagacgg aaaacggcca cgtccatgat gctgcgacta tcgcgggtgc
     6721 ccacgtcata gagcatcgga acgaaaaaat ctggttgctc gtcgcccttg ggcggcttcc
     6781 taatcgacgg cgcaccggct gccggcggtt gccgggattc tttgcggatt cgatcagcgg
     6841 ccgcttgcca cgattcaccg gggcgtgctt ctgcctcgat gcgttgccgc tgggcggcct
     6901 gcgcggcctt caacttctcc accaggtcat cacccagcgc cgcgccgatt tgtaccgggc
     6961 cggatggttt gcgaccgctc acgccgattc ctcgggcttg ggggttccag tgccattgca
     7021 gggccggcag acaacccagc cgcttacgcc tggccaaccg cccgttcctc cacacatggg
     7081 gcattccacg gcgtcggtgc ctggttgttc ttgattttcc atgccgcctc ctttagccgc
     7141 taaaattcat ctactcattt attcatttgc tcatttactc tggtagctgc gcgatgtatt
     7201 cagatagcag ctcggtaatg gtcttgcctt ggcgtaccgc gtacatcttc agcttggtgt
     7261 gatcctccgc cggcaactga aagttgaccc gcttcatggc tggcgtgtct gccaggctgg
     7321 ccaacgttgc agccttgctg ctgcgtgcgc tcggacggcc ggcacttagc gtgtttgtgc
     7381 ttttgctcat tttctcttta cctcattaac tcaaatgagt tttgatttaa tttcagcggc
     7441 cagcgcctgg acctcgcggg cagcgtcgcc ctcgggttct gattcaagaa cggttgtgcc
     7501 ggcggcggca gtgcctgggt agctcacgcg ctgcgtgata cgggactcaa gaatgggcag
     7561 ctcgtacccg gccagcgcct cggcaacctc accgccgatg cgcgtgcctt tgatcgcccg
     7621 cgacacgaca aaggccgctt gtagccttcc atccgtgacc tcaatgcgct gcttaaccag
     7681 ctccaccagg tcggcggtgg cccatatgtc gtaagggctt ggctgcaccg gaatcagcac
     7741 gaagtcggct gccttgatcg cggacacagc caagtccgcc gcctggggcg ctccgtcgat
     7801 cactacgaag tcgcgccggc cgatggcctt cacgtcgcgg tcaatcgtcg ggcggtcgat
     7861 gccgacaacg gttagcggtt gatcttcccg cacggccgcc caatcgcggg cactgccctg
     7921 gggatcggaa tcgactaaca gaacatcggc cccggcgagt tgcagggcgc gggctagatg
     7981 ggttgcgatg gtcgtcttgc ctgacccgcc tttctggtta agtacagcga taaccttcat
     8041 gcgttcccct tgcgtatttg tttatttact catcgcatca tatacgcagc gaccgcatga
     8101 cgcaagctgt tttactcaaa tacacatcac ctttttagac ggcggcgctc ggtttcttca
     8161 gcggccaagc tggccggcca ggccgccagc ttggcatcag acaaaccggc caggatttca
     8221 tgcagccgca cggttgagac gtgcgcgggc ggctcgaaca cgtacccggc cgcgatcatc
     8281 tccgcctcga tctcttcggt aatgaaaaac ggttcgtcct ggccgtcctg gtgcggtttc
     8341 atgcttgttc ctcttggcgt tcattctcgg cggccgccag ggcgtcggcc tcggtcaatg
     8401 cgtcctcacg gaaggcaccg cgccgcctgg cctcggtggg cgtcacttcc tcgctgcgct
     8461 caagtgcgcg gtacagggtc gagcgatgca cgccaagcag tgcagccgcc tctttcacgg
     8521 tgcggccttc ctggtcgatc agctcgcggg cgtgcgcgat ctgtgccggg gtgagggtag
     8581 ggcgggggcc aaacttcacg cctcgggcct tggcggcctc gcgcccgctc cgggtgcggt
     8641 cgatgattag ggaacgctcg aactcggcaa tgccggcgaa cacggtcaac accatgcggc
     8701 cggccggcgt ggtggtgtcg gcccacggct ctgccaggct acgcaggccc gcgccggcct
     8761 cctggatgcg ctcggcaatg tccagtaggt cgcgggtgct gcgggccagg cggtctagcc
     8821 tggtcactgt cacaacgtcg ccagggcgta ggtggtcaag catcctggcc agctccgggc
     8881 ggtcgcgcct ggtgccggtg atcttctcgg aaaacagctt ggtgcagccg gccgcgtgca
     8941 gttcggcccg ttggttggtc aagtcctggt cgtcggtgct gacgcgggca tagcccagca
     9001 ggccagcggc ggcgctcttg ttcatggcgt aatgtctccg gttctagtcg caagtattct
     9061 actttatgcg actaaaacac gcgacaagaa aacgccagga aaagggcagg gcggcagcct
     9121 gtcgcgtaac ttaggacttg tgcgacatgt cgttttcaga agacggctgc actgaacgtc
     9181 agaagccgac tgcactatag cagcggaggg gttggatcaa agtactttga tcccgagggg
     9241 aaccctgtgg ttggcatgca catacaaatg gacgaacgga taaacct
//
