LOCUS       sequence-121804-       13622 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway binary vector designed for transgenic research with 
            Marchantia polymorpha as well as other plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13622)
  AUTHORS   Ishizaki K, Nishihama R, Ueda M, Inoue K, Ishida S, Nishimura Y, 
            Shikanai T, Kohchi T
  TITLE     Development of Gateway Binary Vector Series with Four Different 
            Selection Markers for the Liverwort Marchantia polymorpha.
  JOURNAL   PLoS One. 2015 Sep 25;10(9):e0138876. doi: 
            10.1371/journal.pone.0138876. eCollection 2015.
  PUBMED    26406247
REFERENCE   2  (bases 1 to 13622)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-09-25"; volume: "10(9):e0138876. doi"; pages: " 
            10.1371/journal.pone.0138876. eCollection 2015"
COMMENT     Sequence Label: pMpGWB316
FEATURES             Location/Qualifiers
     source          1..13622
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    54..78
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     266..288
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     280..297
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    323..447
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(484..489,490..507,508..514)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             568..1227
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(634..653)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             1569..1874
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     1753..1772
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(1915..2039)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2050..3477
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGRYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     CDS             3484..3504
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      3529..3781
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(3815..3831)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3815..3831)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3828..3850)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3839..3855
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3863..3869,3870..3887,3888..3893))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3908..3929
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     polyA_signal    4014..4189
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        complement(6273..6949)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     complement(6316..6336)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     misc_feature    7144..7168
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             7689..8480
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      8726..9314
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9215..9234
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9468..9485
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    9500..9640
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(9566..9585)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9726..9748
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      9984..10178
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(10244..11317)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(11746..12375)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgggt ctagagttat caacaagttt gtacaaaaaa gctgaacgag aaacgtaaaa
      361 tgatataaat atcaatatat taaattagat tttgcataaa aaacagacta cataatactg
      421 taaaacacaa catatccagt cactatggcg gcccctcgag gggccgcatt aggcacccca
      481 ggctttacac tttatgcttc cggctcgtat aatgtgtgga ttttgagtta ggatccggcg
      541 agattttcag gagctaagga agctaaaatg gagaaaaaaa tcactggata taccaccgtt
      601 gatatatccc aatggcatcg taaagaacat tttgaggcat ttcagtcagt tgctcaatgt
      661 acctataacc agaccgttca gctggatatt acggcctttt taaagaccgt aaagaaaaat
      721 aagcacaagt tttatccggc ctttattcac attcttgccc gcctgatgaa tgctcatccg
      781 gaattccgta tggcaatgaa agacggtgag ctggtgatat gggatagtgt tcacccttgt
      841 tacaccgttt tccatgagca aactgaaacg ttttcatcgc tctggagtga ataccacgac
      901 gatttccggc agtttctaca catatattcg caagatgtgg cgtgttacgg tgaaaacctg
      961 gcctatttcc ctaaagggtt tattgagaat atgtttttcg tctcagccaa tccctgggtg
     1021 agtttcacca gttttgattt aaacgtggcc aatatggaca acttcttcgc ccccgttttc
     1081 accatgggca aatattatac gcaaggcgac aaggtgctga tgccgctggc gattcaggtt
     1141 catcatgccg tctgtgatgg cttccatgtc ggcagaatgc ttaatgaatt acaacagtac
     1201 tgcgatgagt ggcagggcgg ggcgtaaacg cgtggatccg gcttactaaa agccagataa
     1261 cagtatgcgt atttgcgcgc tgatttttgc ggtataagaa tatatactga tatgtatacc
     1321 cgaagtatgt caaaaagagg tgtgctatga agcagcgtat tacagtgaca gttgacagcg
     1381 acagctatca gttgctcaag gcatatatga tgtcaatatc tccggtctgg taagcacaac
     1441 catgcagaat gaagcccgtc gtctgcgtgc cgaacgctgg aaagcggaaa atcaggaagg
     1501 gatggctgag gtcgcccggt ttattgaaat gaacggctct tttgctgacg agaacaggga
     1561 ctggtgaaat gcagtttaag gtttacacct ataaaagaga gagccgttat cgtctgtttg
     1621 tggatgtaca gagtgatatt attgacacgc ccgggcgacg gatggtgatc cccctggcca
     1681 gtgcacgtct gctgtcagat aaagtctccc gtgaacttta cccggtggtg catatcgggg
     1741 atgaaagctg gcgcatgatg accaccgata tggccagtgt gccggtctcc gttatcgggg
     1801 aagaagtggc tgatctcagc caccgcgaaa atgacatcaa aaacgccatt aacctgatgt
     1861 tctggggaat ataaatgtca ggctccctta tacacagcca gtctgcaggt cgaccatagt
     1921 gactggatat gttgtgtttt acagtattat gtagtctgtt ttttatgcaa aatctaattt
     1981 aatatattga tatttatatc attttacgtt tctcgttcag ctttcttgta caaagtggtt
     2041 gataacagca tggtgagcaa gggcgaggag gtcatcaaag agttcatgcg cttcaaggtg
     2101 cgcatggagg gctccatgaa cggccacgag ttcgagatcg agggcgaggg cgagggccgc
     2161 ccctacgagg gcacccagac cgccaagctg aaggtgacca agggcggccc cctgcccttc
     2221 gcctgggaca tcctgtcccc ccagttcatg tacggctcca aggcgtacgt gaagcacccc
     2281 gccgacatcc ccgattacaa gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg
     2341 atgaacttcg aggacggcgg tctggtgacc gtgacccagg actcctccct gcaggacggc
     2401 acgctgatct acaaggtgaa gatgcgcggc accaacttcc cccccgacgg ccccgtaatg
     2461 cagaagaaga ccatgggctg ggaggcctcc accgagcgcc tgtacccccg cgacggcgtg
     2521 ctgaagggcg agatccacca ggccctgaag ctgaaggacg gcggccacta cctggtggag
     2581 ttcaagacca tctacatggc caagaagccc gtgcaactgc ccggctacta ctacgtggac
     2641 accaagctgg acatcacctc ccacaacgag gactacacca tcgtggaaca gtacgagcgc
     2701 tccgagggcc gccaccacct gttcctgggg catggcaccg gcagcaccgg cagcggcagc
     2761 tccggcaccg cctcctccga ggacaacaac atggccgtca tcaaagagtt catgcgcttc
     2821 aaggtgcgca tggagggctc catgaacggc cacgagttcg agatcgaggg cgagggcgag
     2881 ggccgcccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg cggccccctg
     2941 cccttcgcct gggacatcct gtccccccag ttcatgtacg gctccaaggc gtacgtgaag
     3001 caccccgccg acatccccga ttacaagaag ctgtccttcc ccgagggctt caagtgggag
     3061 cgcgtgatga acttcgagga cggcggtctg gtgaccgtga cccaggactc ctccctgcag
     3121 gacggcacgc tgatctacaa ggtgaagatg cgcggcacca acttcccccc cgacggcccc
     3181 gtaatgcaga agaagaccat gggctgggag gcctccaccg agcgcctgta cccccgcgac
     3241 ggcgtgctga agggcgagat ccaccaggcc ctgaagctga aggacggcgg ccgctacctg
     3301 gtggagttca agaccatcta catggccaag aagcccgtgc aactgcccgg ctactactac
     3361 gtggacacca agctggacat cacctcccac aacgaggact acaccatcgt ggaacagtac
     3421 gagcgctccg agggccgcca ccacctgttc ctgtacggca tggacgagct gtacaagttg
     3481 cagcctaaga agaagagaaa ggttggagga tagagctcga atttccccga tcgttcaaac
     3541 atttggcaat aaagtttctt aagattgaat cctgttgccg gtcttgcgat gattatcata
     3601 taatttctgt tgaattacgt taagcatgta ataattaaca tgtaatgcat gacgttattt
     3661 atgagatggg tttttatgat tagagtcccg caattataca tttaatacgc gatagaaaac
     3721 aaaatatagc gcgcaaacta ggataaatta tcgcgcgcgg tgtcatctat gttactagat
     3781 cgggaattgg ttccggaacc aattcgtaat catggtcata gctgtttcct gtgtgaaatt
     3841 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
     3901 gtgcctaatg agtgagctaa ctcacattag gctgaattag gcgcgcctat ttctgaattc
     3961 aacacattgc ggacgttttt aatgtactga attaacgccg aattaattcg ggggatctgg
     4021 attttagtac tggattttgg ttttaggaat tagaaatttt attgatagaa gtattttaca
     4081 aatacaaata catactaagg gtttcttata tgctcaacac atgagcgaaa ccctatagga
     4141 accctaaatt cccttatctg ggaactactc acacattatt atggagaaac tcgaagttag
     4201 tatatagtcc ttccatcacc ctctgtgata atctccttaa aacttgctcc cccaggaatc
     4261 ataggcagaa cgtgctcctg gtggggaact atcacatcca gtaaataagg accgggagaa
     4321 tctagcatag tttgaattgc tgctcttagt tcatccttct tcaccacacg cgcatttggc
     4381 accttgaaac cgtctgcaac tgtacagaag tcggggtaga tctgctcctc gtgatctgga
     4441 tcacctaagt aagtatgtgc tctgttggcc ttgtagaatc tatcctccaa ttgtacgacc
     4501 attcctagat gttggttgtt taagatcatg atcttcacgg gtaacttctc cacaaaaatt
     4561 gtagccaact cctgtacgtt catcacaaag ctaccatcgc catcgatgtc gatcacagga
     4621 atatctggtt gagttgcggc agcccccaaa gcagaaggaa gtccgaatcc catggctcca
     4681 agacctccag atgtcaacca acgccgcggt tcgttgtact tgaaccactg ggctgcccac
     4741 atttgatgtt ggccaacacc agtagatatg atggcatttc caccagtgag ttcgtacagc
     4801 gtctgtatgg cagattgagg agcaatggca tcctcgaata cgggaaactt cattggccat
     4861 ttctgctttt gttcttctag ttctgccacc cagttggaga agtcgaaagt cctcttttga
     4921 ctcttcaaga gggcattgat ttcacttagc gcaggtttca catcagcaca catggacaca
     4981 tggggctgtt tgttctttcc aatttcagca gggtcaatat cgatatgaac gatgctggac
     5041 ctgctagcaa aagtctccag tttaccagtc actcgatcgt cgaaacgcac tccaaatgcc
     5101 aacaacagat ctgcttggtc cacggcgtag ttggcgtaaa cagttccatg cattccaagc
     5161 attttcaaac ttaatggatc agactcggga tacgctccca aacccattaa agtcgatgtg
     5221 ataggaattc cggtaagctc gacaaattcc ctcaactctt cgctggcgtg attgcaacct
     5281 cctccagaat acactacagg cttcttagaa ctagaaatga gtcttataat ctgctcgatt
     5341 aacgttggtt ccggtggccg aggcagtcgt tgtaagtagc ccttcagttg cattggctga
     5401 gaccaatctg gaatagccat ttgctgttgc acgtccttgg gaatatctac caagactggg
     5461 cccggcctac ctgaagctgc caaaaagaaa gcctccctga ttacgcgagg gatgtcatca
     5521 atgttcataa caaggtaatt gtgcttggtg atcgatctcg taacctccac aattggagtt
     5581 tcttgaaaag catcggtacc aatcatccta ctggatactt gacctgtaat ggccactagt
     5641 ggcacactgt ccaagagggc atcagcgagc ccggtaacaa gattggtcgc tccaggaccg
     5701 gatgtggcta tacaaacccc tactcgtcca gacgatttag cataaccttc agcagcgaaa
     5761 atctccccct gctcgtggcg acagagaatg ttgcggatgc tgctggaacg tgtaagagcc
     5821 tggtgaatct ccatagacgc tccgccggga taagcgaaca ccgttctaat cccttctcgc
     5881 tcaagagcct ccaccaaaat gtcggctcct ttgcggggct cgtcatcctt gtagcgagac
     5941 tgaaagtcgg acggggttgg tgcaacgagt gacttcccgc tgctaagccg tggtccactc
     6001 acatcgactg cagactggat cttctcgcta acagcttggg ctttcacttc cagtgaacgc
     6061 tcagtcttcc gagaggtgtt gtatgcatgt cccctcagag cggctcccga gaagaaacca
     6121 ctcctcacct ttagctcttt agctcccttg caacaagacc ttgccgatgg ctgcaccttc
     6181 tccaggctcg ctatgctcaa aggcacagct gcgcatccac taattgacgc catgctcgag
     6241 agagatagat ttgtagagag agactggtga tttcagcgtg tcctctccaa atgaaatgaa
     6301 cttccttata tagaggaagg gtcttgcgaa ggatagtggg attgtgcgtc atcccttacg
     6361 tcagtggaga tatcacatca atccacttgc tttgaagacg tggttggaac gtcttctttt
     6421 tccacgatgc tcctcgtggg tgggggtcca tctttgggac cactgtcggc agaggcatct
     6481 tgaacgatag cctttccttt atcgcaatta tggcatttgt aggtgccacc ttccttttct
     6541 actgtccttt tgatgaagtg acagatagct gggcaatgga atccgaggag gtttcccgat
     6601 attacccttt gttgaaaagt ctcaatagcc ctttggtctt ctgagactgt atctttgata
     6661 ttcttggagt agacgagagt gtcgtgctcc accatgttca catcaatcca cttgctttga
     6721 agacgtggtt ggaacgtctt ctttttccac gatgctcctc gtgggtgggg gtccatcttt
     6781 gggaccactg tcggcagagg catcttgaac gatagccttt cctttatcgc aatgatggca
     6841 tttgtaggtg ccaccttcct tttctactgt ccttttgatg aagtgacaga tagctgggca
     6901 atggaatccg aggaggtttc ccgatattac cctttgttga aaagtctcaa tagccctttg
     6961 gtcttctgag actgtatctt tgatattctt ggagtagacg agagtgtcgt gctccaccat
     7021 gttggcaagc tgctctagcc aatacgcaaa ccgaattcag aaataaattc agcctaattc
     7081 ggcgttaatt cagtacatta aaaacgtccg caatgtgtta ttaagttgtc taagcgtcaa
     7141 tttgtttaca ccacaatata tcctgccacc agccagccaa cagctccccg accggcagct
     7201 cggcacaaaa tcaccactcg atacaggcag cccatcagtc cgggacggcg tcagcgggag
     7261 agccgttgta aggcggcaga ctttgctcat gttaccgatg ctattcggaa gaacggcaac
     7321 taagctgccg ggtttgaaac acggatgatc tcgcggaggg tagcatgttg attgtaacga
     7381 tgacagagcg ttgctgcctg tgatcaattc gggcacgaac ccagtggaca taagcctgtt
     7441 cggttcgtaa gctgtaatgc aagtagcgta tgcgctcacg caactggtcc agaaccttga
     7501 ccgaacgcag cggtggtaac ggcgcagtgg cggttttcat ggcttgttat gactgttttt
     7561 ttggggtaca gtctatgcct cgggcatcca agcagcaagc gcgttacgcc gtgggtcgat
     7621 gtttgatgtt atggagcagc aacgatgtta cgcagcaggg cagtcgccct aaaacaaagt
     7681 taaacatcat gggggaagcg gtgatcgccg aagtatcgac tcaactatca gaggtagttg
     7741 gcgtcatcga gcgccatctc gaaccgacgt tgctggccgt acatttgtac ggctccgcag
     7801 tggatggcgg cctgaagcca cacagtgata ttgatttgct ggttacggtg accgtaaggc
     7861 ttgatgaaac aacgcggcga gctttgatca acgacctttt ggaaacttcg gcttcccctg
     7921 gagagagcga gattctccgc gctgtagaag tcaccattgt tgtgcacgac gacatcattc
     7981 cgtggcgtta tccagctaag cgcgaactgc aatttggaga atggcagcgc aatgacattc
     8041 ttgcaggtat cttcgagcca gccacgatcg acattgatct ggctatcttg ctgacaaaag
     8101 caagagaaca tagcgttgcc ttggtaggtc cagcggcgga ggaactcttt gatccggttc
     8161 ctgaacagga tctatttgag gcgctaaatg aaaccttaac gctatggaac tcgccgcccg
     8221 actgggctgg cgatgagcga aatgtagtgc ttacgttgtc ccgcatttgg tacagcgcag
     8281 taaccggcaa aatcgcgccg aaggatgtcg ctgccgactg ggcaatggag cgcctgccgg
     8341 cccagtatca gcccgtcata cttgaagcta gacaggctta tcttggacaa gaagaagatc
     8401 gcttggcctc gcgcgcagat cagttggaag aatttgtcca ctacgtgaaa ggcgagatca
     8461 ccaaggtagt cggcaaataa tgtctagcta gaaattcgtt caagccgacg ccgcttcgcg
     8521 gcgcggctta actcaagcgt tagatgcact aagcacataa ttgctcacag ccaaactatc
     8581 aggtcaagtc tgcttttatt atttttaagc gtgcataata agccctacac aaattgggag
     8641 atatatcatg catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     8701 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     8761 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     8821 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag
     8881 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     8941 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     9001 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     9061 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     9121 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     9181 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     9241 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     9301 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     9361 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     9421 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     9481 gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt
     9541 cacaccgcat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt
     9601 atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc
     9661 cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac
     9721 cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca
     9781 gggtgccttg atgtgggcgc cggcggtcga gtggcgacgg cgcggcttgt ccgcgccctg
     9841 gtagattgcc tggccgtagg ccagccattt ttgagcggcc agcggccgcg ataggccgac
     9901 gcgaagcggc ggggcgtagg gagcgcagcg accgaagggt aggcgctttt tgcagctctt
     9961 cggctgtgcg ctggccagac agttatgcac aggccaggcg ggttttaaga gttttaataa
    10021 gttttaaaga gttttaggcg gaaaaatcgc cttttttctc ttttatatca gtcacttaca
    10081 tgtgtgaccg gttcccaatg tacggctttg ggttcccaat gtacgggttc cggttcccaa
    10141 tgtacggctt tgggttccca atgtacgtgc tatccacagg aaagagacct tttcgacctt
    10201 tttcccctgc tagggcaatt tgccctagca tctgctccgt acattaggaa ccggcggatg
    10261 cttcgccctc gatcaggttg cggtagcgca tgactaggat cgggccagcc tgccccgcct
    10321 cctccttcaa atcgtactcc ggcaggtcat ttgacccgat cagcttgcgc acggtgaaac
    10381 agaacttctt gaactctccg gcgctgccac tgcgttcgta gatcgtcttg aacaaccatc
    10441 tggcttctgc cttgcctgcg gcgcggcgtg ccaggcggta gagaaaacgg ccgatgccgg
    10501 gatcgatcaa aaagtaatcg gggtgaaccg tcagcacgtc cgggttcttg ccttctgtga
    10561 tctcgcggta catccaatca gctagctcga tctcgatgta ctccggccgc ccggtttcgc
    10621 tctttacgat cttgtagcgg ctaatcaagg cttcaccctc ggataccgtc accaggcggc
    10681 cgttcttggc cttcttcgta cgctgcatgg caacgtgcgt ggtgtttaac cgaatgcagg
    10741 tttctaccag gtcgtctttc tgctttccgc catcggctcg ccggcagaac ttgagtacgt
    10801 ccgcaacgtg tggacggaac acgcggccgg gcttgtctcc cttcccttcc cggtatcggt
    10861 tcatggattc ggttagatgg gaaaccgcca tcagtaccag gtcgtaatcc cacacactgg
    10921 ccatgccggc cggccctgcg gaaacctcta cgtgcccgtc tggaagctcg tagcggatca
    10981 cctcgccagc tcgtcggtca cgcttcgaca gacggaaaac ggccacgtcc atgatgctgc
    11041 gactatcgcg ggtgcccacg tcatagagca tcggaacgaa aaaatctggt tgctcgtcgc
    11101 ccttgggcgg cttcctaatc gacggcgcac cggctgccgg cggttgccgg gattctttgc
    11161 ggattcgatc agcggccgct tgccacgatt caccggggcg tgcttctgcc tcgatgcgtt
    11221 gccgctgggc ggcctgcgcg gccttcaact tctccaccag gtcatcaccc agcgccgcgc
    11281 cgatttgtac cgggccggat ggtttgcgac cgctcacgcc gattcctcgg gcttgggggt
    11341 tccagtgcca ttgcagggcc ggcagacaac ccagccgctt acgcctggcc aaccgcccgt
    11401 tcctccacac atggggcatt ccacggcgtc ggtgcctggt tgttcttgat tttccatgcc
    11461 gcctccttta gccgctaaaa ttcatctact catttattca tttgctcatt tactctggta
    11521 gctgcgcgat gtattcagat agcagctcgg taatggtctt gccttggcgt accgcgtaca
    11581 tcttcagctt ggtgtgatcc tccgccggca actgaaagtt gacccgcttc atggctggcg
    11641 tgtctgccag gctggccaac gttgcagcct tgctgctgcg tgcgctcgga cggccggcac
    11701 ttagcgtgtt tgtgcttttg ctcattttct ctttacctca ttaactcaaa tgagttttga
    11761 tttaatttca gcggccagcg cctggacctc gcgggcagcg tcgccctcgg gttctgattc
    11821 aagaacggtt gtgccggcgg cggcagtgcc tgggtagctc acgcgctgcg tgatacggga
    11881 ctcaagaatg ggcagctcgt acccggccag cgcctcggca acctcaccgc cgatgcgcgt
    11941 gcctttgatc gcccgcgaca cgacaaaggc cgcttgtagc cttccatccg tgacctcaat
    12001 gcgctgctta accagctcca ccaggtcggc ggtggcccat atgtcgtaag ggcttggctg
    12061 caccggaatc agcacgaagt cggctgcctt gatcgcggac acagccaagt ccgccgcctg
    12121 gggcgctccg tcgatcacta cgaagtcgcg ccggccgatg gccttcacgt cgcggtcaat
    12181 cgtcgggcgg tcgatgccga caacggttag cggttgatct tcccgcacgg ccgcccaatc
    12241 gcgggcactg ccctggggat cggaatcgac taacagaaca tcggccccgg cgagttgcag
    12301 ggcgcgggct agatgggttg cgatggtcgt cttgcctgac ccgcctttct ggttaagtac
    12361 agcgataacc ttcatgcgtt ccccttgcgt atttgtttat ttactcatcg catcatatac
    12421 gcagcgaccg catgacgcaa gctgttttac tcaaatacac atcacctttt tagacggcgg
    12481 cgctcggttt cttcagcggc caagctggcc ggccaggccg ccagcttggc atcagacaaa
    12541 ccggccagga tttcatgcag ccgcacggtt gagacgtgcg cgggcggctc gaacacgtac
    12601 ccggccgcga tcatctccgc ctcgatctct tcggtaatga aaaacggttc gtcctggccg
    12661 tcctggtgcg gtttcatgct tgttcctctt ggcgttcatt ctcggcggcc gccagggcgt
    12721 cggcctcggt caatgcgtcc tcacggaagg caccgcgccg cctggcctcg gtgggcgtca
    12781 cttcctcgct gcgctcaagt gcgcggtaca gggtcgagcg atgcacgcca agcagtgcag
    12841 ccgcctcttt cacggtgcgg ccttcctggt cgatcagctc gcgggcgtgc gcgatctgtg
    12901 ccggggtgag ggtagggcgg gggccaaact tcacgcctcg ggccttggcg gcctcgcgcc
    12961 cgctccgggt gcggtcgatg attagggaac gctcgaactc ggcaatgccg gcgaacacgg
    13021 tcaacaccat gcggccggcc ggcgtggtgg tgtcggccca cggctctgcc aggctacgca
    13081 ggcccgcgcc ggcctcctgg atgcgctcgg caatgtccag taggtcgcgg gtgctgcggg
    13141 ccaggcggtc tagcctggtc actgtcacaa cgtcgccagg gcgtaggtgg tcaagcatcc
    13201 tggccagctc cgggcggtcg cgcctggtgc cggtgatctt ctcggaaaac agcttggtgc
    13261 agccggccgc gtgcagttcg gcccgttggt tggtcaagtc ctggtcgtcg gtgctgacgc
    13321 gggcatagcc cagcaggcca gcggcggcgc tcttgttcat ggcgtaatgt ctccggttct
    13381 agtcgcaagt attctacttt atgcgactaa aacacgcgac aagaaaacgc caggaaaagg
    13441 gcagggcggc agcctgtcgc gtaacttagg acttgtgcga catgtcgttt tcagaagacg
    13501 gctgcactga acgtcagaag ccgactgcac tatagcagcg gaggggttgg atcaaagtac
    13561 tttgatcccg aggggaaccc tgtggttggc atgcacatac aaatggacga acggataaac
    13621 ct
//
