LOCUS       sequence-122002-         346 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  express Fis1 tail-anchored FRB for heterodimerization with FKBP in 
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 346)
  AUTHORS   Narendra D, Kane LA, Hauser DN, Fearnley IM, Youle RJ
  TITLE     p62/SQSTM1 is required for Parkin-induced mitochondrial clustering 
            but not mitophagy; VDAC1 is dispensable for both.
  JOURNAL   Autophagy. 2010 Nov;6(8):1090-106.
  PUBMED    20890124
REFERENCE   2  (bases 1 to 346)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Autophagy."; date: "2010-11"; volume: "6(8)"; pages: "1090-106"
COMMENT     Sequence Label: pC4-RhE-FRB-Fis1
FEATURES             Location/Qualifiers
     source          1..346
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      46..55
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             64..342
                     /codon_start=1
                     /product="T2098L mutant of FKBP-rapamycin binding domain of
                     human FRAP"
                     /label=FRB*
                     /note="binds synthetic ligands such as AP21967"
                     /translation="ILWHEMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTL
                     KETSFNQAYGRDLMEAQEWCRKYMKSGNVKDLLQAWDLYYHVFRRISK"
ORIGIN
        1 ggcgtgaaac tcccgcacct cttcggccag cgaattccag aagccgccac catggcttct
       61 agaatcctct ggcatgagat gtggcatgaa ggcctggaag aggcatctcg tttgtacttt
      121 ggggaaagga acgtgaaagg catgtttgag gtgctggagc ccttgcatgc tatgatggaa
      181 cggggccccc agactctgaa ggaaacatcc tttaatcagg cctatggtcg agatttaatg
      241 gaggcccaag agtggtgcag gaagtacatg aaatcaggga atgtcaagga cctcctccaa
      301 gcctgggacc tctattatca tgtgttccga cgaatctcaa agacta
//
