LOCUS       sequence-122004-         677 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  cyclic nucleotide reporter for bacteria or plant cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 677)
  AUTHORS   Wheeler JI, Freihat L, Irving HR
  TITLE     A cyclic nucleotide sensitive promoter reporter system suitable for 
            bacteria and plant cells.
  JOURNAL   BMC Biotechnol. 2013 Nov 9;13:97. doi: 10.1186/1472-6750-13-97.
  PUBMED    24206622
REFERENCE   2  (bases 1 to 677)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2013-11-9"; volume: "13:97. doi"; pages: " 
            10.1186/1472-6750-13-97"
COMMENT     Sequence Label: pOPTXGARE
FEATURES             Location/Qualifiers
     source          1..677
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    40..64
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             625..642
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gccgggcctt tctttatgtt tttggcgtct tccatggcaa ccactttgta caagaaagct
       61 gggtccatag ctcttttctt tttttcttgt attatgtcaa acaaaggaag atataagttt
      121 ggctttagga gttggcacaa agttaaatta actaggtggg gagcgaggag gtacttataa
      181 tgtctcgttc atttttaaaa gaatcatgaa agagaaacta gaaaaatatt atccaatatt
      241 aatcaactat ttctacattc tctttgttag gctctctttg ttaggctctc tttgttaggc
      301 tctctttgtt aggctctctt tgttaggaat ttttgttaga aactagactt caatatcaat
      361 atccaataaa tataaatctt tctaataaaa agtaaaaaaa attcttaaat caacttttgt
      421 gtaaaaagag aaacaatgaa ttcttataga gaagttcatt tttaaaatta tggttttagt
      481 tattanaaaa atatcaaaat actattgaat aaattgtgta actaatttaa aacttaaata
      541 tatcaaataa ttaagtttac taaacttctt agtgtattag agtagtggga agaaatggac
      601 ccactgtcca atatgtttaa aatgcatcat catcatcatc attacaagaa aatttcaact
      661 ttggcctgtg gctctgg
//
