LOCUS       sequence-122051-       15237 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway binary vector designed for transgenic research with 
            Marchantia polymorpha as well as other plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15237)
  AUTHORS   Ishizaki K, Nishihama R, Ueda M, Inoue K, Ishida S, Nishimura Y, 
            Shikanai T, Kohchi T
  TITLE     Development of Gateway Binary Vector Series with Four Different 
            Selection Markers for the Liverwort Marchantia polymorpha.
  JOURNAL   PLoS One. 2015 Sep 25;10(9):e0138876. doi: 
            10.1371/journal.pone.0138876. eCollection 2015.
  PUBMED    26406247
REFERENCE   2  (bases 1 to 15237)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-09-25"; volume: "10(9):e0138876. doi"; pages: " 
            10.1371/journal.pone.0138876. eCollection 2015"
COMMENT     Sequence Label: pMpGWB325
FEATURES             Location/Qualifiers
     source          1..15237
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    54..78
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     266..288
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     280..297
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        812..1157
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    1179..1303
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(1340..1345,1346..1363,1364..1370)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             1424..2083
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1490..1509)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             2425..2730
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     2609..2628
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(2771..2895)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             join(2927..2929,2930..2932,2933..3643)
                     /codon_start=1
                     /product="enhanced variant of YFP (Heikal et al., 2001)"
                     /label=Citrine
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             3446..4183
                     /codon_start=1
                     /product="circularly permuted form of the Venus yellow 
                     fluorescent protein (Nagai et al., 2004)"
                     /label=cp173Venus
                     /note="efficient FRET acceptor for CFP"
                     /translation="DGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYKAGGSGGSMVSKGEELFTGVVPILVELDGDVNGHKFSVS
                     GEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMP
                     EGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSH
                     NVYIMADKQKNGIKVNFKIRHNIE"
     CDS             join(3665..3667,3668..3670,3671..4381)
                     /codon_start=1
                     /product="enhanced variant of YFP (Heikal et al., 2001)"
                     /label=Citrine
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             4184..4921
                     /codon_start=1
                     /product="circularly permuted form of the Venus yellow 
                     fluorescent protein (Nagai et al., 2004)"
                     /label=cp173Venus
                     /note="efficient FRET acceptor for CFP"
                     /translation="DGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYKAGGSGGSMVSKGEELFTGVVPILVELDGDVNGHKFSVS
                     GEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMP
                     EGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSH
                     NVYIMADKQKNGIKVNFKIRHNIE"
     CDS             join(4403..4405,4406..4408,4409..5119)
                     /codon_start=1
                     /product="enhanced variant of YFP (Heikal et al., 2001)"
                     /label=Citrine
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     terminator      5144..5396
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(5430..5446)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5430..5446)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5443..5465)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5454..5470
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5478..5484,5485..5502,5503..5508))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5523..5544
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     polyA_signal    5629..5804
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        complement(7888..8564)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     misc_feature    8759..8783
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             9304..10095
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      10341..10929
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10830..10849
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11083..11100
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    11115..11255
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(11181..11200)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     11341..11363
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      11599..11793
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(11859..12932)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(13361..13990)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgcat gcctgcaggt ccccagatta gccttttcaa tttcagaaag aatgctaacc
      361 cacagatggt tagagaggct tacgcagcag gtctcatcaa gacgatctac ccgagcaata
      421 atctccagga aatcaaatac cttcccaaga aggttaaaga tgcagtcaaa agattcagga
      481 ctaactgcat caagaacaca gagaaagata tatttctcaa gatcagaagt actattccag
      541 tatggacgat tcaaggcttg cttcacaaac caaggcaagt aatagagatt ggagtctcta
      601 aaaaggtagt tcccactgaa tcaaaggcca tggagtcaaa gattcaaata gaggacctaa
      661 cagaactcgc cgtaaagact ggcgaacagt tcatacagag tctcttacga ctcaatgaca
      721 agaagaaaat cttcgtcaac atggtggagc acgacacact tgtctactcc aaaaatatca
      781 aagatacagt ctcagaagac caaagggcaa ttgagacttt tcaacaaagg gtaatatccg
      841 gaaacctcct cggattccat tgcccagcta tctgtcactt tattgtgaag atagtggaaa
      901 aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg aaaggccatc gttgaagatg
      961 cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag
     1021 aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa
     1081 gggatgacgc acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat
     1141 ttcatttgga gagaacacgg gggactctag agttatcaac aagtttgtac aaaaaagctg
     1201 aacgagaaac gtaaaatgat ataaatatca atatattaaa ttagattttg cataaaaaac
     1261 agactacata atactgtaaa acacaacata tccagtcact atggcggccc ctcgaggggc
     1321 cgcattaggc accccaggct ttacacttta tgcttccggc tcgtataatg tgtggatttt
     1381 gagttaggat ccgtcgagat tttcaggagc taaggaagct aaaatggaga aaaaaatcac
     1441 tggatatacc accgttgata tatcccaatg gcatcgtaaa gaacattttg aggcatttca
     1501 gtcagttgct caatgtacct ataaccagac cgttcagctg gatattacgg cctttttaaa
     1561 gaccgtaaag aaaaataagc acaagtttta tccggccttt attcacattc ttgcccgcct
     1621 gatgaatgct catccggaat tccgtatggc aatgaaagac ggtgagctgg tgatatggga
     1681 tagtgttcac ccttgttaca ccgttttcca tgagcaaact gaaacgtttt catcgctctg
     1741 gagtgaatac cacgacgatt tccggcagtt tctacacata tattcgcaag atgtggcgtg
     1801 ttacggtgaa aacctggcct atttccctaa agggtttatt gagaatatgt ttttcgtctc
     1861 agccaatccc tgggtgagtt tcaccagttt tgatttaaac gtggccaata tggacaactt
     1921 cttcgccccc gttttcacca tgggcaaata ttatacgcaa ggcgacaagg tgctgatgcc
     1981 gctggcgatt caggttcatc atgccgtttg tgatggcttc catgtcggca gaatgcttaa
     2041 tgaattacaa cagtactgcg atgagtggca gggcggggcg taaagatctg gatccggctt
     2101 actaaaagcc agataacagt atgcgtattt gcgcgctgat ttttgcggta taagaatata
     2161 tactgatatg tatacccgaa gtatgtcaaa aagaggtatg ctatgaagca gcgtattaca
     2221 gtgacagttg acagcgacag ctatcagttg ctcaaggcat atatgatgtc aatatctccg
     2281 gtctggtaag cacaaccatg cagaatgaag cccgtcgtct gcgtgccgaa cgctggaaag
     2341 cggaaaatca ggaagggatg gctgaggtcg cccggtttat tgaaatgaac ggctcttttg
     2401 ctgacgagaa caggggctgg tgaaatgcag tttaaggttt acacctataa aagagagagc
     2461 cgttatcgtc tgtttgtgga tgtacagagt gatattattg acacgcccgg gcgacggatg
     2521 gtgatccccc tggccagtgc acgtctgctg tcagataaag tcccccgtga actttacccg
     2581 gtggtgcata tcggggatga aagctggcgc atgatgacca ccgatatggc cagtgtgccg
     2641 gtctccgtta tcggggaaga agtggctgat ctcagccacc gcgaaaatga catcaaaaac
     2701 gccattaacc tgatgttctg gggaatataa atgtcaggct cccttataca cagccagtct
     2761 gcaggtcgac catagtgact ggatatgttg tgttttacag tattatgtag tctgtttttt
     2821 atgcaaaatc taatttaata tattgatatt tatatcattt tacgtttctc gttcagcttt
     2881 cttgtacaaa gtggttgata acagcgctgg aggctccggt gggagcatgg tgagcaaggg
     2941 cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg
     3001 ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct
     3061 gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccacctt
     3121 cggctacggc ctgatgtgct tcgcccgcta ccccgaccac atgaagcagc acgacttctt
     3181 caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg
     3241 caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga
     3301 gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa
     3361 ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa
     3421 cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca
     3481 gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagctacca
     3541 gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt
     3601 gaccgccgcc gggatcactc tcggcatgga cgagctgtac aaggctggag gctccggtgg
     3661 gagcatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct
     3721 ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac
     3781 ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc
     3841 caccctcgtg accaccttcg gctacggcct gatgtgcttc gcccgctacc ccgaccacat
     3901 gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat
     3961 cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac
     4021 cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg
     4081 gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa
     4141 gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct
     4201 cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa
     4261 ccactacctg agctaccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat
     4321 ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa
     4381 ggctggaggc tccggtggga gcatggtgag caagggcgag gagctgttca ccggggtggt
     4441 gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga
     4501 gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa
     4561 gctgcccgtg ccctggccca ccctcgtgac caccttcggc tacggcctga tgtgcttcgc
     4621 ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta
     4681 cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt
     4741 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga
     4801 ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat
     4861 catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga
     4921 ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc
     4981 cgtgctgctg cccgacaacc actacctgag ctaccagtcc gccctgagca aagaccccaa
     5041 cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg
     5101 catggacgag ctgtacaagt aagcttagag ctcgaatttc cccgatcgtt caaacatttg
     5161 gcaataaagt ttcttaagat tgaatcctgt tgccggtctt gcgatgatta tcatataatt
     5221 tctgttgaat tacgttaagc atgtaataat taacatgtaa tgcatgacgt tatttatgag
     5281 atgggttttt atgattagag tcccgcaatt atacatttaa tacgcgatag aaaacaaaat
     5341 atagcgcgca aactaggata aattatcgcg cgcggtgtca tctatgttac tagatcggga
     5401 attggttccg gaaccaattc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
     5461 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
     5521 taatgagtga gctaactcac attaggctga attaggcgcg cctatttctg aattcaacac
     5581 attgcggacg tttttaatgt actgaattaa cgccgaatta attcggggga tctggatttt
     5641 agtactggat tttggtttta ggaattagaa attttattga tagaagtatt ttacaaatac
     5701 aaatacatac taagggtttc ttatatgctc aacacatgag cgaaacccta taggaaccct
     5761 aaattccctt atctgggaac tactcacaca ttattatgga gaaactcgaa gttagtatat
     5821 agtccttcca tcaccctctg tgataatctc cttaaaactt gctcccccag gaatcatagg
     5881 cagaacgtgc tcctggtggg gaactatcac atccagtaaa taaggaccgg gagaatctag
     5941 catagtttga attgctgctc ttagttcatc cttcttcacc acacgcgcat ttggcacctt
     6001 gaaaccgtct gcaactgtac agaagtcggg gtagatctgc tcctcgtgat ctggatcacc
     6061 taagtaagta tgtgctctgt tggccttgta gaatctatcc tccaattgta cgaccattcc
     6121 tagatgttgg ttgtttaaga tcatgatctt cacgggtaac ttctccacaa aaattgtagc
     6181 caactcctgt acgttcatca caaagctacc atcgccatcg atgtcgatca caggaatatc
     6241 tggttgagtt gcggcagccc ccaaagcaga aggaagtccg aatcccatgg ctccaagacc
     6301 tccagatgtc aaccaacgcc gcggttcgtt gtacttgaac cactgggctg cccacatttg
     6361 atgttggcca acaccagtag atatgatggc atttccacca gtgagttcgt acagcgtctg
     6421 tatggcagat tgaggagcaa tggcatcctc gaatacggga aacttcattg gccatttctg
     6481 cttttgttct tctagttctg ccacccagtt ggagaagtcg aaagtcctct tttgactctt
     6541 caagagggca ttgatttcac ttagcgcagg tttcacatca gcacacatgg acacatgggg
     6601 ctgtttgttc tttccaattt cagcagggtc aatatcgata tgaacgatgc tggacctgct
     6661 agcaaaagtc tccagtttac cagtcactcg atcgtcgaaa cgcactccaa atgccaacaa
     6721 cagatctgct tggtccacgg cgtagttggc gtaaacagtt ccatgcattc caagcatttt
     6781 caaacttaat ggatcagact cgggatacgc tcccaaaccc attaaagtcg atgtgatagg
     6841 aattccggta agctcgacaa attccctcaa ctcttcgctg gcgtgattgc aacctcctcc
     6901 agaatacact acaggcttct tagaactaga aatgagtctt ataatctgct cgattaacgt
     6961 tggttccggt ggccgaggca gtcgttgtaa gtagcccttc agttgcattg gctgagacca
     7021 atctggaata gccatttgct gttgcacgtc cttgggaata tctaccaaga ctgggcccgg
     7081 cctacctgaa gctgccaaaa agaaagcctc cctgattacg cgagggatgt catcaatgtt
     7141 cataacaagg taattgtgct tggtgatcga tctcgtaacc tccacaattg gagtttcttg
     7201 aaaagcatcg gtaccaatca tcctactgga tacttgacct gtaatggcca ctagtggcac
     7261 actgtccaag agggcatcag cgagcccggt aacaagattg gtcgctccag gaccggatgt
     7321 ggctatacaa acccctactc gtccagacga tttagcataa ccttcagcag cgaaaatctc
     7381 cccctgctcg tggcgacaga gaatgttgcg gatgctgctg gaacgtgtaa gagcctggtg
     7441 aatctccata gacgctccgc cgggataagc gaacaccgtt ctaatccctt ctcgctcaag
     7501 agcctccacc aaaatgtcgg ctcctttgcg gggctcgtca tccttgtagc gagactgaaa
     7561 gtcggacggg gttggtgcaa cgagtgactt cccgctgcta agccgtggtc cactcacatc
     7621 gactgcagac tggatcttct cgctaacagc ttgggctttc acttccagtg aacgctcagt
     7681 cttccgagag gtgttgtatg catgtcccct cagagcggct cccgagaaga aaccactcct
     7741 cacctttagc tctttagctc ccttgcaaca agaccttgcc gatggctgca ccttctccag
     7801 gctcgctatg ctcaaaggca cagctgcgca tccactaatt gacgccatgc tcgagagaga
     7861 tagatttgta gagagagact ggtgatttca gcgtgtcctc tccaaatgaa atgaacttcc
     7921 ttatatagag gaagggtctt gcgaaggata gtgggattgt gcgtcatccc ttacgtcagt
     7981 ggagatatca catcaatcca cttgctttga agacgtggtt ggaacgtctt ctttttccac
     8041 gatgctcctc gtgggtgggg gtccatcttt gggaccactg tcggcagagg catcttgaac
     8101 gatagccttt cctttatcgc aattatggca tttgtaggtg ccaccttcct tttctactgt
     8161 ccttttgatg aagtgacaga tagctgggca atggaatccg aggaggtttc ccgatattac
     8221 cctttgttga aaagtctcaa tagccctttg gtcttctgag actgtatctt tgatattctt
     8281 ggagtagacg agagtgtcgt gctccaccat gttcacatca atccacttgc tttgaagacg
     8341 tggttggaac gtcttctttt tccacgatgc tcctcgtggg tgggggtcca tctttgggac
     8401 cactgtcggc agaggcatct tgaacgatag cctttccttt atcgcaatga tggcatttgt
     8461 aggtgccacc ttccttttct actgtccttt tgatgaagtg acagatagct gggcaatgga
     8521 atccgaggag gtttcccgat attacccttt gttgaaaagt ctcaatagcc ctttggtctt
     8581 ctgagactgt atctttgata ttcttggagt agacgagagt gtcgtgctcc accatgttgg
     8641 caagctgctc tagccaatac gcaaaccgaa ttcagaaata aattcagcct aattcggcgt
     8701 taattcagta cattaaaaac gtccgcaatg tgttattaag ttgtctaagc gtcaatttgt
     8761 ttacaccaca atatatcctg ccaccagcca gccaacagct ccccgaccgg cagctcggca
     8821 caaaatcacc actcgataca ggcagcccat cagtccggga cggcgtcagc gggagagccg
     8881 ttgtaaggcg gcagactttg ctcatgttac cgatgctatt cggaagaacg gcaactaagc
     8941 tgccgggttt gaaacacgga tgatctcgcg gagggtagca tgttgattgt aacgatgaca
     9001 gagcgttgct gcctgtgatc aattcgggca cgaacccagt ggacataagc ctgttcggtt
     9061 cgtaagctgt aatgcaagta gcgtatgcgc tcacgcaact ggtccagaac cttgaccgaa
     9121 cgcagcggtg gtaacggcgc agtggcggtt ttcatggctt gttatgactg tttttttggg
     9181 gtacagtcta tgcctcgggc atccaagcag caagcgcgtt acgccgtggg tcgatgtttg
     9241 atgttatgga gcagcaacga tgttacgcag cagggcagtc gccctaaaac aaagttaaac
     9301 atcatggggg aagcggtgat cgccgaagta tcgactcaac tatcagaggt agttggcgtc
     9361 atcgagcgcc atctcgaacc gacgttgctg gccgtacatt tgtacggctc cgcagtggat
     9421 ggcggcctga agccacacag tgatattgat ttgctggtta cggtgaccgt aaggcttgat
     9481 gaaacaacgc ggcgagcttt gatcaacgac cttttggaaa cttcggcttc ccctggagag
     9541 agcgagattc tccgcgctgt agaagtcacc attgttgtgc acgacgacat cattccgtgg
     9601 cgttatccag ctaagcgcga actgcaattt ggagaatggc agcgcaatga cattcttgca
     9661 ggtatcttcg agccagccac gatcgacatt gatctggcta tcttgctgac aaaagcaaga
     9721 gaacatagcg ttgccttggt aggtccagcg gcggaggaac tctttgatcc ggttcctgaa
     9781 caggatctat ttgaggcgct aaatgaaacc ttaacgctat ggaactcgcc gcccgactgg
     9841 gctggcgatg agcgaaatgt agtgcttacg ttgtcccgca tttggtacag cgcagtaacc
     9901 ggcaaaatcg cgccgaagga tgtcgctgcc gactgggcaa tggagcgcct gccggcccag
     9961 tatcagcccg tcatacttga agctagacag gcttatcttg gacaagaaga agatcgcttg
    10021 gcctcgcgcg cagatcagtt ggaagaattt gtccactacg tgaaaggcga gatcaccaag
    10081 gtagtcggca aataatgtct agctagaaat tcgttcaagc cgacgccgct tcgcggcgcg
    10141 gcttaactca agcgttagat gcactaagca cataattgct cacagccaaa ctatcaggtc
    10201 aagtctgctt ttattatttt taagcgtgca taataagccc tacacaaatt gggagatata
    10261 tcatgcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
    10321 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
    10381 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
    10441 tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag
    10501 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
    10561 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
    10621 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
    10681 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
    10741 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
    10801 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
    10861 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
    10921 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
    10981 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
    11041 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
    11101 gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac
    11161 cgcatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca
    11221 ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg
    11281 acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct
    11341 ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagggtg
    11401 ccttgatgtg ggcgccggcg gtcgagtggc gacggcgcgg cttgtccgcg ccctggtaga
    11461 ttgcctggcc gtaggccagc catttttgag cggccagcgg ccgcgatagg ccgacgcgaa
    11521 gcggcggggc gtagggagcg cagcgaccga agggtaggcg ctttttgcag ctcttcggct
    11581 gtgcgctggc cagacagtta tgcacaggcc aggcgggttt taagagtttt aataagtttt
    11641 aaagagtttt aggcggaaaa atcgcctttt ttctctttta tatcagtcac ttacatgtgt
    11701 gaccggttcc caatgtacgg ctttgggttc ccaatgtacg ggttccggtt cccaatgtac
    11761 ggctttgggt tcccaatgta cgtgctatcc acaggaaaga gaccttttcg acctttttcc
    11821 cctgctaggg caatttgccc tagcatctgc tccgtacatt aggaaccggc ggatgcttcg
    11881 ccctcgatca ggttgcggta gcgcatgact aggatcgggc cagcctgccc cgcctcctcc
    11941 ttcaaatcgt actccggcag gtcatttgac ccgatcagct tgcgcacggt gaaacagaac
    12001 ttcttgaact ctccggcgct gccactgcgt tcgtagatcg tcttgaacaa ccatctggct
    12061 tctgccttgc ctgcggcgcg gcgtgccagg cggtagagaa aacggccgat gccgggatcg
    12121 atcaaaaagt aatcggggtg aaccgtcagc acgtccgggt tcttgccttc tgtgatctcg
    12181 cggtacatcc aatcagctag ctcgatctcg atgtactccg gccgcccggt ttcgctcttt
    12241 acgatcttgt agcggctaat caaggcttca ccctcggata ccgtcaccag gcggccgttc
    12301 ttggccttct tcgtacgctg catggcaacg tgcgtggtgt ttaaccgaat gcaggtttct
    12361 accaggtcgt ctttctgctt tccgccatcg gctcgccggc agaacttgag tacgtccgca
    12421 acgtgtggac ggaacacgcg gccgggcttg tctcccttcc cttcccggta tcggttcatg
    12481 gattcggtta gatgggaaac cgccatcagt accaggtcgt aatcccacac actggccatg
    12541 ccggccggcc ctgcggaaac ctctacgtgc ccgtctggaa gctcgtagcg gatcacctcg
    12601 ccagctcgtc ggtcacgctt cgacagacgg aaaacggcca cgtccatgat gctgcgacta
    12661 tcgcgggtgc ccacgtcata gagcatcgga acgaaaaaat ctggttgctc gtcgcccttg
    12721 ggcggcttcc taatcgacgg cgcaccggct gccggcggtt gccgggattc tttgcggatt
    12781 cgatcagcgg ccgcttgcca cgattcaccg gggcgtgctt ctgcctcgat gcgttgccgc
    12841 tgggcggcct gcgcggcctt caacttctcc accaggtcat cacccagcgc cgcgccgatt
    12901 tgtaccgggc cggatggttt gcgaccgctc acgccgattc ctcgggcttg ggggttccag
    12961 tgccattgca gggccggcag acaacccagc cgcttacgcc tggccaaccg cccgttcctc
    13021 cacacatggg gcattccacg gcgtcggtgc ctggttgttc ttgattttcc atgccgcctc
    13081 ctttagccgc taaaattcat ctactcattt attcatttgc tcatttactc tggtagctgc
    13141 gcgatgtatt cagatagcag ctcggtaatg gtcttgcctt ggcgtaccgc gtacatcttc
    13201 agcttggtgt gatcctccgc cggcaactga aagttgaccc gcttcatggc tggcgtgtct
    13261 gccaggctgg ccaacgttgc agccttgctg ctgcgtgcgc tcggacggcc ggcacttagc
    13321 gtgtttgtgc ttttgctcat tttctcttta cctcattaac tcaaatgagt tttgatttaa
    13381 tttcagcggc cagcgcctgg acctcgcggg cagcgtcgcc ctcgggttct gattcaagaa
    13441 cggttgtgcc ggcggcggca gtgcctgggt agctcacgcg ctgcgtgata cgggactcaa
    13501 gaatgggcag ctcgtacccg gccagcgcct cggcaacctc accgccgatg cgcgtgcctt
    13561 tgatcgcccg cgacacgaca aaggccgctt gtagccttcc atccgtgacc tcaatgcgct
    13621 gcttaaccag ctccaccagg tcggcggtgg cccatatgtc gtaagggctt ggctgcaccg
    13681 gaatcagcac gaagtcggct gccttgatcg cggacacagc caagtccgcc gcctggggcg
    13741 ctccgtcgat cactacgaag tcgcgccggc cgatggcctt cacgtcgcgg tcaatcgtcg
    13801 ggcggtcgat gccgacaacg gttagcggtt gatcttcccg cacggccgcc caatcgcggg
    13861 cactgccctg gggatcggaa tcgactaaca gaacatcggc cccggcgagt tgcagggcgc
    13921 gggctagatg ggttgcgatg gtcgtcttgc ctgacccgcc tttctggtta agtacagcga
    13981 taaccttcat gcgttcccct tgcgtatttg tttatttact catcgcatca tatacgcagc
    14041 gaccgcatga cgcaagctgt tttactcaaa tacacatcac ctttttagac ggcggcgctc
    14101 ggtttcttca gcggccaagc tggccggcca ggccgccagc ttggcatcag acaaaccggc
    14161 caggatttca tgcagccgca cggttgagac gtgcgcgggc ggctcgaaca cgtacccggc
    14221 cgcgatcatc tccgcctcga tctcttcggt aatgaaaaac ggttcgtcct ggccgtcctg
    14281 gtgcggtttc atgcttgttc ctcttggcgt tcattctcgg cggccgccag ggcgtcggcc
    14341 tcggtcaatg cgtcctcacg gaaggcaccg cgccgcctgg cctcggtggg cgtcacttcc
    14401 tcgctgcgct caagtgcgcg gtacagggtc gagcgatgca cgccaagcag tgcagccgcc
    14461 tctttcacgg tgcggccttc ctggtcgatc agctcgcggg cgtgcgcgat ctgtgccggg
    14521 gtgagggtag ggcgggggcc aaacttcacg cctcgggcct tggcggcctc gcgcccgctc
    14581 cgggtgcggt cgatgattag ggaacgctcg aactcggcaa tgccggcgaa cacggtcaac
    14641 accatgcggc cggccggcgt ggtggtgtcg gcccacggct ctgccaggct acgcaggccc
    14701 gcgccggcct cctggatgcg ctcggcaatg tccagtaggt cgcgggtgct gcgggccagg
    14761 cggtctagcc tggtcactgt cacaacgtcg ccagggcgta ggtggtcaag catcctggcc
    14821 agctccgggc ggtcgcgcct ggtgccggtg atcttctcgg aaaacagctt ggtgcagccg
    14881 gccgcgtgca gttcggcccg ttggttggtc aagtcctggt cgtcggtgct gacgcgggca
    14941 tagcccagca ggccagcggc ggcgctcttg ttcatggcgt aatgtctccg gttctagtcg
    15001 caagtattct actttatgcg actaaaacac gcgacaagaa aacgccagga aaagggcagg
    15061 gcggcagcct gtcgcgtaac ttaggacttg tgcgacatgt cgttttcaga agacggctgc
    15121 actgaacgtc agaagccgac tgcactatag cagcggaggg gttggatcaa agtactttga
    15181 tcccgagggg aaccctgtgg ttggcatgca catacaaatg gacgaacgga taaacct
//
