LOCUS       sequence-122054-       11447 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway binary vector designed for transgenic research with 
            Marchantia polymorpha as well as other plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11447)
  AUTHORS   Ishizaki K, Nishihama R, Ueda M, Inoue K, Ishida S, Nishimura Y, 
            Shikanai T, Kohchi T
  TITLE     Development of Gateway Binary Vector Series with Four Different 
            Selection Markers for the Liverwort Marchantia polymorpha.
  JOURNAL   PLoS One. 2015 Sep 25;10(9):e0138876. doi: 
            10.1371/journal.pone.0138876. eCollection 2015.
  PUBMED    26406247
REFERENCE   2  (bases 1 to 11447)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-09-25"; volume: "10(9):e0138876. doi"; pages: " 
            10.1371/journal.pone.0138876. eCollection 2015"
COMMENT     Sequence Label: pMpGWB226
FEATURES             Location/Qualifiers
     source          1..11447
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    54..78
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     266..288
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     280..297
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    323..447
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(484..489,490..507,508..514)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             568..1227
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(634..653)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             1569..1874
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     1753..1772
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(1915..2039)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2071..2766
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRSDMALK
                     LVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAVARYC
                     DLPSKLGHK"
     terminator      2788..3040
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(3074..3090)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3074..3090)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3087..3109)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3098..3114
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3122..3128,3129..3146,3147..3152))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3167..3188
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     polyA_signal    3273..3445
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(3502..4035)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MVRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(4098..4774)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     complement(4141..4161)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     misc_feature    4969..4993
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             5514..6305
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      6551..7139
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7040..7059
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7293..7310
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    7325..7465
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(7391..7410)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7551..7573
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      7809..8003
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(8069..9142)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(9571..10200)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgggt ctagagttat caacaagttt gtacaaaaaa gctgaacgag aaacgtaaaa
      361 tgatataaat atcaatatat taaattagat tttgcataaa aaacagacta cataatactg
      421 taaaacacaa catatccagt cactatggcg gcccctcgag gggccgcatt aggcacccca
      481 ggctttacac tttatgcttc cggctcgtat aatgtgtgga ttttgagtta ggatccgtcg
      541 agattttcag gagctaagga agctaaaatg gagaaaaaaa tcactggata taccaccgtt
      601 gatatatccc aatggcatcg taaagaacat tttgaggcat ttcagtcagt tgctcaatgt
      661 acctataacc agaccgttca gctggatatt acggcctttt taaagaccgt aaagaaaaat
      721 aagcacaagt tttatccggc ctttattcac attcttgccc gcctgatgaa tgctcatccg
      781 gaattccgta tggcaatgaa agacggtgag ctggtgatat gggatagtgt tcacccttgt
      841 tacaccgttt tccatgagca aactgaaacg ttttcatcgc tctggagtga ataccacgac
      901 gatttccggc agtttctaca catatattcg caagatgtgg cgtgttacgg tgaaaacctg
      961 gcctatttcc ctaaagggtt tattgagaat atgtttttcg tctcagccaa tccctgggtg
     1021 agtttcacca gttttgattt aaacgtggcc aatatggaca acttcttcgc ccccgttttc
     1081 accatgggca aatattatac gcaaggcgac aaggtgctga tgccgctggc gattcaggtt
     1141 catcatgccg tttgtgatgg cttccatgtc ggcagaatgc ttaatgaatt acaacagtac
     1201 tgcgatgagt ggcagggcgg ggcgtaaaga tctggatccg gcttactaaa agccagataa
     1261 cagtatgcgt atttgcgcgc tgatttttgc ggtataagaa tatatactga tatgtatacc
     1321 cgaagtatgt caaaaagagg tatgctatga agcagcgtat tacagtgaca gttgacagcg
     1381 acagctatca gttgctcaag gcatatatga tgtcaatatc tccggtctgg taagcacaac
     1441 catgcagaat gaagcccgtc gtctgcgtgc cgaacgctgg aaagcggaaa atcaggaagg
     1501 gatggctgag gtcgcccggt ttattgaaat gaacggctct tttgctgacg agaacagggg
     1561 ctggtgaaat gcagtttaag gtttacacct ataaaagaga gagccgttat cgtctgtttg
     1621 tggatgtaca gagtgatatt attgacacgc ccgggcgacg gatggtgatc cccctggcca
     1681 gtgcacgtct gctgtcagat aaagtccccc gtgaacttta cccggtggtg catatcgggg
     1741 atgaaagctg gcgcatgatg accaccgata tggccagtgt gccggtctcc gttatcgggg
     1801 aagaagtggc tgatctcagc caccgcgaaa atgacatcaa aaacgccatt aacctgatgt
     1861 tctggggaat ataaatgtca ggctccctta tacacagcca gtctgcaggt cgaccatagt
     1921 gactggatat gttgtgtttt acagtattat gtagtctgtt ttttatgcaa aatctaattt
     1981 aatatattga tatttatatc attttacgtt tctcgttcag ctttcttgta caaagtggtt
     2041 gataacagcg ctggaggctc cggtgggagc atgagcgagc tgattaagga gaacatgcac
     2101 atgaagctgt acatggaggg caccgtgaac aaccaccact tcaagtgcac atccgagggc
     2161 gaaggcaagc cctacgaggg cacccagacc atgagaatca aggtggtcga gggcggccct
     2221 ctccccttcg ccttcgacat cctggctacc agcttcatgt acggcagcag aaccttcatc
     2281 aaccacaccc agggcatccc cgacttcttt aagcagtcct tccctgaggg cttcacatgg
     2341 gagagagtca ccacatacga agacgggggc gtgctgaccg ctacccagga caccagcctc
     2401 caggacggct gcctcatcta caacgtcaag atcagagggg tgaacttccc atccaacggc
     2461 cctgtgatgc agaagaaaac actcggctgg gaggccaaca ccgagatgct gtaccccgct
     2521 gacggcggcc tggaaggcag aagcgacatg gccctgaagc tcgtgggcgg gggccacctg
     2581 atctgcaact tcaagaccac atacagatcc aagaaacccg ctaagaacct caagatgccc
     2641 ggcgtctact atgtggacca cagactggaa agaatcaagg aggccgacaa agagacctac
     2701 gtcgagcagc acgaggtggc tgtggccaga tactgcgacc tccctagcaa actggggcac
     2761 aagtgagctt agagctcgaa tttccccgat cgttcaaaca tttggcaata aagtttctta
     2821 agattgaatc ctgttgccgg tcttgcgatg attatcatat aatttctgtt gaattacgtt
     2881 aagcatgtaa taattaacat gtaatgcatg acgttattta tgagatgggt ttttatgatt
     2941 agagtcccgc aattatacat ttaatacgcg atagaaaaca aaatatagcg cgcaaactag
     3001 gataaattat cgcgcgcggt gtcatctatg ttactagatc gggaattggt tccggaacca
     3061 attcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
     3121 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
     3181 tcacattagg ctgaattagg cgcgcctatt tctgaattca acacattgcg gacgttttta
     3241 atgtactgaa ttaacgccga attaattcgg gggatctgga ttttagtact ggattttggt
     3301 tttaggaatt agattttatt gatagaagta ttttacaaat acaaatacat actaagggtt
     3361 tcttatatgc tcaacacatg agcgaaaccc tataggaacc ctaattccct tatctgggaa
     3421 ctactcacac attattatgg agaaactcga gcttgtcgat cgactctagg ccgggaagcc
     3481 gatctcggct tgaacgaatt gttaggtggc ggtacttggg tcgatatcaa agtgcatcac
     3541 ttcttcccgt atgcccaact ttgtatagag agccactgcg ggatcgtcac cgtaatctgc
     3601 ttgcacgtag atcacataag caccaagcgc gttggcctca tgcttgagga gattgatgag
     3661 cgcggtggca atgccctgcc tccggtgctc gccggagact gcgagatcat agatatagat
     3721 ctcactacgc ggctgctcaa acttgggcag aacgtaagcc gcgagagcgc caacaaccgc
     3781 ttcttggtcg aaggcagcaa gcgcgatgaa tgtcttacta cggagcaagt tcccgaggta
     3841 atcggagtcc ggctgatgtt gggagtaggt ggctacgtct ccgaactcac gaccgaaaag
     3901 atcaagagca gcccgcatgg atttgacttg gtcagggccg agcctacatg tgcgaatgat
     3961 gcccatactt gagccaccta actttgtttt agggcgactg ccctgctgcg taacatcgtt
     4021 gctgctgcgt accatggtcg atcgacagat ctgcgaaagc tcgagagaga tagatttgta
     4081 gagagagact ggtgatttca gcgtgtcctc tccaaatgaa atgaacttcc ttatatagag
     4141 gaagggtctt gcgaaggata gtgggattgt gcgtcatccc ttacgtcagt ggagatatca
     4201 catcaatcca cttgctttga agacgtggtt ggaacgtctt ctttttccac gatgctcctc
     4261 gtgggtgggg gtccatcttt gggaccactg tcggcagagg catcttgaac gatagccttt
     4321 cctttatcgc aatgatggca tttgtaggtg ccaccttcct tttctactgt ccttttgatg
     4381 aagtgacaga tagctgggca atggaatccg aggaggtttc ccgatattac cctttgttga
     4441 aaagtctcaa tagccctttg gtcttctgag actgtatctt tgatattctt ggagtagacg
     4501 agagtgtcgt gctccaccat gttcacatca atccacttgc tttgaagacg tggttggaac
     4561 gtcttctttt tccacgatgc tcctcgtggg tgggggtcca tctttgggac cactgtcggc
     4621 agaggcatct tgaacgatag cctttccttt atcgcaatga tggcatttgt aggtgccacc
     4681 ttccttttct actgtccttt tgatgaagtg acagatagct gggcaatgga atccgaggag
     4741 gtttcccgat attacccttt gttgaaaagt ctcaatagcc ctttggtctt ctgagactgt
     4801 atctttgata ttcttggagt agacgagagt gtcgtgctcc accatgttgg caagctgctc
     4861 tagccaatac gcaaaccgaa ttcagaaata aattcagcct aattcggcgt taattcagta
     4921 cattaaaaac gtccgcaatg tgttattaag ttgtctaagc gtcaatttgt ttacaccaca
     4981 atatatcctg ccaccagcca gccaacagct ccccgaccgg cagctcggca caaaatcacc
     5041 actcgataca ggcagcccat cagtccggga cggcgtcagc gggagagccg ttgtaaggcg
     5101 gcagactttg ctcatgttac cgatgctatt cggaagaacg gcaactaagc tgccgggttt
     5161 gaaacacgga tgatctcgcg gagggtagca tgttgattgt aacgatgaca gagcgttgct
     5221 gcctgtgatc aattcgggca cgaacccagt ggacataagc ctgttcggtt cgtaagctgt
     5281 aatgcaagta gcgtatgcgc tcacgcaact ggtccagaac cttgaccgaa cgcagcggtg
     5341 gtaacggcgc agtggcggtt ttcatggctt gttatgactg tttttttggg gtacagtcta
     5401 tgcctcgggc atccaagcag caagcgcgtt acgccgtggg tcgatgtttg atgttatgga
     5461 gcagcaacga tgttacgcag cagggcagtc gccctaaaac aaagttaaac atcatggggg
     5521 aagcggtgat cgccgaagta tcgactcaac tatcagaggt agttggcgtc atcgagcgcc
     5581 atctcgaacc gacgttgctg gccgtacatt tgtacggctc cgcagtggat ggcggcctga
     5641 agccacacag tgatattgat ttgctggtta cggtgaccgt aaggcttgat gaaacaacgc
     5701 ggcgagcttt gatcaacgac cttttggaaa cttcggcttc ccctggagag agcgagattc
     5761 tccgcgctgt agaagtcacc attgttgtgc acgacgacat cattccgtgg cgttatccag
     5821 ctaagcgcga actgcaattt ggagaatggc agcgcaatga cattcttgca ggtatcttcg
     5881 agccagccac gatcgacatt gatctggcta tcttgctgac aaaagcaaga gaacatagcg
     5941 ttgccttggt aggtccagcg gcggaggaac tctttgatcc ggttcctgaa caggatctat
     6001 ttgaggcgct aaatgaaacc ttaacgctat ggaactcgcc gcccgactgg gctggcgatg
     6061 agcgaaatgt agtgcttacg ttgtcccgca tttggtacag cgcagtaacc ggcaaaatcg
     6121 cgccgaagga tgtcgctgcc gactgggcaa tggagcgcct gccggcccag tatcagcccg
     6181 tcatacttga agctagacag gcttatcttg gacaagaaga agatcgcttg gcctcgcgcg
     6241 cagatcagtt ggaagaattt gtccactacg tgaaaggcga gatcaccaag gtagtcggca
     6301 aataatgtct agctagaaat tcgttcaagc cgacgccgct tcgcggcgcg gcttaactca
     6361 agcgttagat gcactaagca cataattgct cacagccaaa ctatcaggtc aagtctgctt
     6421 ttattatttt taagcgtgca taataagccc tacacaaatt gggagatata tcatgcatga
     6481 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     6541 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     6601 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     6661 taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag
     6721 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     6781 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     6841 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     6901 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     6961 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     7021 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     7081 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     7141 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     7201 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
     7261 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
     7321 agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatggt
     7381 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc
     7441 gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg
     7501 acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg
     7561 catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagggtg ccttgatgtg
     7621 ggcgccggcg gtcgagtggc gacggcgcgg cttgtccgcg ccctggtaga ttgcctggcc
     7681 gtaggccagc catttttgag cggccagcgg ccgcgatagg ccgacgcgaa gcggcggggc
     7741 gtagggagcg cagcgaccga agggtaggcg ctttttgcag ctcttcggct gtgcgctggc
     7801 cagacagtta tgcacaggcc aggcgggttt taagagtttt aataagtttt aaagagtttt
     7861 aggcggaaaa atcgcctttt ttctctttta tatcagtcac ttacatgtgt gaccggttcc
     7921 caatgtacgg ctttgggttc ccaatgtacg ggttccggtt cccaatgtac ggctttgggt
     7981 tcccaatgta cgtgctatcc acaggaaaga gaccttttcg acctttttcc cctgctaggg
     8041 caatttgccc tagcatctgc tccgtacatt aggaaccggc ggatgcttcg ccctcgatca
     8101 ggttgcggta gcgcatgact aggatcgggc cagcctgccc cgcctcctcc ttcaaatcgt
     8161 actccggcag gtcatttgac ccgatcagct tgcgcacggt gaaacagaac ttcttgaact
     8221 ctccggcgct gccactgcgt tcgtagatcg tcttgaacaa ccatctggct tctgccttgc
     8281 ctgcggcgcg gcgtgccagg cggtagagaa aacggccgat gccgggatcg atcaaaaagt
     8341 aatcggggtg aaccgtcagc acgtccgggt tcttgccttc tgtgatctcg cggtacatcc
     8401 aatcagctag ctcgatctcg atgtactccg gccgcccggt ttcgctcttt acgatcttgt
     8461 agcggctaat caaggcttca ccctcggata ccgtcaccag gcggccgttc ttggccttct
     8521 tcgtacgctg catggcaacg tgcgtggtgt ttaaccgaat gcaggtttct accaggtcgt
     8581 ctttctgctt tccgccatcg gctcgccggc agaacttgag tacgtccgca acgtgtggac
     8641 ggaacacgcg gccgggcttg tctcccttcc cttcccggta tcggttcatg gattcggtta
     8701 gatgggaaac cgccatcagt accaggtcgt aatcccacac actggccatg ccggccggcc
     8761 ctgcggaaac ctctacgtgc ccgtctggaa gctcgtagcg gatcacctcg ccagctcgtc
     8821 ggtcacgctt cgacagacgg aaaacggcca cgtccatgat gctgcgacta tcgcgggtgc
     8881 ccacgtcata gagcatcgga acgaaaaaat ctggttgctc gtcgcccttg ggcggcttcc
     8941 taatcgacgg cgcaccggct gccggcggtt gccgggattc tttgcggatt cgatcagcgg
     9001 ccgcttgcca cgattcaccg gggcgtgctt ctgcctcgat gcgttgccgc tgggcggcct
     9061 gcgcggcctt caacttctcc accaggtcat cacccagcgc cgcgccgatt tgtaccgggc
     9121 cggatggttt gcgaccgctc acgccgattc ctcgggcttg ggggttccag tgccattgca
     9181 gggccggcag acaacccagc cgcttacgcc tggccaaccg cccgttcctc cacacatggg
     9241 gcattccacg gcgtcggtgc ctggttgttc ttgattttcc atgccgcctc ctttagccgc
     9301 taaaattcat ctactcattt attcatttgc tcatttactc tggtagctgc gcgatgtatt
     9361 cagatagcag ctcggtaatg gtcttgcctt ggcgtaccgc gtacatcttc agcttggtgt
     9421 gatcctccgc cggcaactga aagttgaccc gcttcatggc tggcgtgtct gccaggctgg
     9481 ccaacgttgc agccttgctg ctgcgtgcgc tcggacggcc ggcacttagc gtgtttgtgc
     9541 ttttgctcat tttctcttta cctcattaac tcaaatgagt tttgatttaa tttcagcggc
     9601 cagcgcctgg acctcgcggg cagcgtcgcc ctcgggttct gattcaagaa cggttgtgcc
     9661 ggcggcggca gtgcctgggt agctcacgcg ctgcgtgata cgggactcaa gaatgggcag
     9721 ctcgtacccg gccagcgcct cggcaacctc accgccgatg cgcgtgcctt tgatcgcccg
     9781 cgacacgaca aaggccgctt gtagccttcc atccgtgacc tcaatgcgct gcttaaccag
     9841 ctccaccagg tcggcggtgg cccatatgtc gtaagggctt ggctgcaccg gaatcagcac
     9901 gaagtcggct gccttgatcg cggacacagc caagtccgcc gcctggggcg ctccgtcgat
     9961 cactacgaag tcgcgccggc cgatggcctt cacgtcgcgg tcaatcgtcg ggcggtcgat
    10021 gccgacaacg gttagcggtt gatcttcccg cacggccgcc caatcgcggg cactgccctg
    10081 gggatcggaa tcgactaaca gaacatcggc cccggcgagt tgcagggcgc gggctagatg
    10141 ggttgcgatg gtcgtcttgc ctgacccgcc tttctggtta agtacagcga taaccttcat
    10201 gcgttcccct tgcgtatttg tttatttact catcgcatca tatacgcagc gaccgcatga
    10261 cgcaagctgt tttactcaaa tacacatcac ctttttagac ggcggcgctc ggtttcttca
    10321 gcggccaagc tggccggcca ggccgccagc ttggcatcag acaaaccggc caggatttca
    10381 tgcagccgca cggttgagac gtgcgcgggc ggctcgaaca cgtacccggc cgcgatcatc
    10441 tccgcctcga tctcttcggt aatgaaaaac ggttcgtcct ggccgtcctg gtgcggtttc
    10501 atgcttgttc ctcttggcgt tcattctcgg cggccgccag ggcgtcggcc tcggtcaatg
    10561 cgtcctcacg gaaggcaccg cgccgcctgg cctcggtggg cgtcacttcc tcgctgcgct
    10621 caagtgcgcg gtacagggtc gagcgatgca cgccaagcag tgcagccgcc tctttcacgg
    10681 tgcggccttc ctggtcgatc agctcgcggg cgtgcgcgat ctgtgccggg gtgagggtag
    10741 ggcgggggcc aaacttcacg cctcgggcct tggcggcctc gcgcccgctc cgggtgcggt
    10801 cgatgattag ggaacgctcg aactcggcaa tgccggcgaa cacggtcaac accatgcggc
    10861 cggccggcgt ggtggtgtcg gcccacggct ctgccaggct acgcaggccc gcgccggcct
    10921 cctggatgcg ctcggcaatg tccagtaggt cgcgggtgct gcgggccagg cggtctagcc
    10981 tggtcactgt cacaacgtcg ccagggcgta ggtggtcaag catcctggcc agctccgggc
    11041 ggtcgcgcct ggtgccggtg atcttctcgg aaaacagctt ggtgcagccg gccgcgtgca
    11101 gttcggcccg ttggttggtc aagtcctggt cgtcggtgct gacgcgggca tagcccagca
    11161 ggccagcggc ggcgctcttg ttcatggcgt aatgtctccg gttctagtcg caagtattct
    11221 actttatgcg actaaaacac gcgacaagaa aacgccagga aaagggcagg gcggcagcct
    11281 gtcgcgtaac ttaggacttg tgcgacatgt cgttttcaga agacggctgc actgaacgtc
    11341 agaagccgac tgcactatag cagcggaggg gttggatcaa agtactttga tcccgagggg
    11401 aaccctgtgg ttggcatgca catacaaatg gacgaacgga taaacct
//
