LOCUS       sequence-122103-        1131 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid for the generation of gene disruptions via Bxb1-mediated 
            recombination into MIN-tagged cell lines. This vector can also be 
            used to express cDNAs..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1131)
  AUTHORS   Mulholland CB, Smets M, Schmidtmann E, Leidescher S, Markaki Y, 
            Hofweber M, Qin W, Manzo M, Kremmer E, Thanisch K, Bauer C, Rombaut 
            P, Herzog F, Leonhardt H, Bultmann S
  TITLE     A modular open platform for systematic functional studies under 
            physiological conditions.
  JOURNAL   Nucleic Acids Res. 2015 May 24. pii: gkv550.
  PUBMED    26007658
REFERENCE   2  (bases 1 to 1131)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2015 May 24. pii: gkv550."
COMMENT     Sequence Label: attB-mCh-Poly(A)
FEATURES             Location/Qualifiers
     source          1..1131
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     597..616
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(698..745)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     polyA_signal    881..1015
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(918..937)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     972..991
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     regulatory      1068..1077
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ccaacgctat gtcctgatag cggtccgcca cacccagccg gccacagtcg atgaatccag
       61 aaaagcggcc attttccacc atgatattcg gcaagcaggc atcgccatgg gtcacgacga
      121 gatcctcgcc gccgggcatg cgcgccttga gcctggcgaa cagttcggct ggcgcgagcc
      181 cctgatgctc ttcgtccaga tcatcctgat cgacaagacc ggcttccatc cgagtacgtg
      241 ctcgctcgat gcgatgtttc gcttggtggt cgaatgggca ggtagccgga tcaagcgtat
      301 gcagccgccg cattgcatca gccatgatgg atactttctc ggcaggagca aggtgagatg
      361 acaggagatc ctgccccggc acttcgccca atagcagcca gtcccttccc gcttcagtga
      421 caacgtcgag cacagctgcg caaggaacgc ccgtcgtggc cagccacgat agccgcgctg
      481 cctcgtcctg cagttcattc agggcaccgg acaggtcggt cttgacaaaa agaaccgggc
      541 gcccctgcgc tgacagccgg aacacggcgg catcagagca gccgattgtc tgttgtgccc
      601 agtcatagcc gaatagcctc tccacccaag cggccggaga acctgcgtgc aatccatctt
      661 gttcaatggc cgatcccatg gtttagttcc tcaccttgtc gtattatact atgccgatat
      721 actatgccga tgattaattg tcaacagaat aaaccgcggc gacgaggctt gcaggatcat
      781 aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc
      841 cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta
      901 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
      961 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatcctcta
     1021 gagaattcgt taactgcggc cgcactagtc aattcccggg cgatcgccca tggtggcgtc
     1081 cacggcgctg ttgctcgcct gggctctgct cctcaggccg ctcttgtaca g
//
