LOCUS       sequence-122197-         442 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Level 1 Golden Gate Cassette: sgRNA cassette targeting PM19_1 in 
            barley.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 442)
  AUTHORS   Lawrenson T, Shorinola O, Stacey N, Li C, Ostergaard L, Patron N, 
            Uauy C, Harwood W
  TITLE     Induction of targeted, heritable mutations in barley and Brassica 
            oleracea using RNA-guided Cas9 nuclease.
  JOURNAL   Genome Biol. 2015 Nov 30;16(1):258. doi: 10.1186/s13059-015-0826-7.
  PUBMED    26616834
REFERENCE   2  (bases 1 to 442)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome 
            Biol."; date: "2015-11-30"; volume: "16(1):258. doi"; pages: " 
            10.1186/s13059-015-0826-7"
COMMENT     Sequence Label: pICSL11057
FEATURES             Location/Qualifiers
     source          1..442
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 aggaccaagc ccgttattct gacagttctg gtgctcaaca catttatatt tatcaaggag
       61 cacattgtta ctcactgcta ggagggaatc gaactaggaa tattgatcag aggaactacg
      121 agagagctga agataactgc cctctagctc tcactgatct gggtcgcata gtgagatgca
      181 gcccacgtga gttcagcaac ggtctagcgc tgggctttta ggcccgcatg atcgggcttt
      241 tgtcgggtgg tcgacgtgtt cacgattggg gagagcaacg cagcagttcc tcttagttta
      301 gtcccacctc gcctgtccag cagagttctg accggtttat aaactcgctt gctgcatcag
      361 acttgctctc cactctgggc tcttgtttta gagctagaaa tagcaagtta aaataaggct
      421 agtccgttat caacttgaaa aa
//
