LOCUS       sequence-122203-         457 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Level 1 Golden Gate Cassette: sgRNA cassette targeting PM19_3 in 
            barley.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 457)
  AUTHORS   Lawrenson T, Shorinola O, Stacey N, Li C, Ostergaard L, Patron N, 
            Uauy C, Harwood W
  TITLE     Induction of targeted, heritable mutations in barley and Brassica 
            oleracea using RNA-guided Cas9 nuclease.
  JOURNAL   Genome Biol. 2015 Nov 30;16(1):258. doi: 10.1186/s13059-015-0826-7.
  PUBMED    26616834
REFERENCE   2  (bases 1 to 457)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome 
            Biol."; date: "2015-11-30"; volume: "16(1):258. doi"; pages: " 
            10.1186/s13059-015-0826-7"
COMMENT     Sequence Label: pICSL11058
FEATURES             Location/Qualifiers
     source          1..457
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    18..42
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_RNA        complement(54..129)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 acaatctgag cgtaatgcca actttgtaca agaaagctgg gtctagaaaa aaagcaccga
       61 ctcggtgcca ctttttcaag ttgataacgg actagcctta ttttaacttg ctatttctag
      121 ctctaaaact gttacgacca acgccagcca agtctgatgc agcaagcgag tttataaacc
      181 ggtcagaact ctgctggaca ggcgaggtgg gactaaacta anaggaactg ctgcgttgct
      241 ctccccaatc gtgaacacgt cgaccacccg acaaaagccc gatcatgcgg gcctaaaagc
      301 ccagcgctag accgttgctg anctcacgtg ggctgcatct cactatgcga cccagatcag
      361 tgagagctag agggcagtta tcttcagctc tctcgtagtt cctctgatca atattcctag
      421 ttcgattccc tcctagcagt gagtaacaat gtgctcc
//
