LOCUS       sequence-122226-         497 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Level 1 Golden Gate Cassette: sgRNA cassette targeting BolC.GA4a-1 
            in Brassica oleracea.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 497)
  AUTHORS   Lawrenson T, Shorinola O, Stacey N, Li C, Ostergaard L, Patron N, 
            Uauy C, Harwood W
  TITLE     Induction of targeted, heritable mutations in barley and Brassica 
            oleracea using RNA-guided Cas9 nuclease.
  JOURNAL   Genome Biol. 2015 Nov 30;16(1):258. doi: 10.1186/s13059-015-0826-7.
  PUBMED    26616834
REFERENCE   2  (bases 1 to 497)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome 
            Biol."; date: "2015-11-30"; volume: "16(1):258. doi"; pages: " 
            10.1186/s13059-015-0826-7"
COMMENT     Sequence Label: pICSL11061
FEATURES             Location/Qualifiers
     source          1..497
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    25..49
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_RNA        complement(61..136)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    470..494
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 gcagaagaca agtaaagcgt aatgccaact ttgtacaaga aagctgggtc tagaaaaaaa
       61 gcaccgactc ggtgccactt tttcaagttg ataacggact agccttattt taacttgcta
      121 tttctagctc taaaacctcc ggccgcaagt gaaaacaatc actacttcga ctctagctgt
      181 atataaactc agcttcgttt tcttatctaa gcgatgtggg acttttgaag attgttttca
      241 acttaaatgg gcctatataa gaaatactat tgttctttcc catataaatg ggcctgcttc
      301 tcttctttca gattcccagg ggccttttga agattatctt catatcttaa gaatgaagat
      361 gctccgagct cgaattctag tttgtcttca cagagtgggg cccactgcat ccaccccagt
      421 acattaaaaa cgtccgcaat gtgttattaa gttgtctaag cgtcaatttg tttacaccac
      481 aatatatcct gccacca
//
