LOCUS       sequence-12225-e        8372 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8372)
  AUTHORS   Huang HH, Camsund D, Lindblad P, Heidorn T
  TITLE     Design and characterization of molecular tools for a Synthetic 
            Biology approach towards developing cyanobacterial biotechnology.
  JOURNAL   Nucleic Acids Res. 2010 May . 38(8):2577-93.
  PUBMED    20236988
REFERENCE   2  (bases 1 to 8372)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2010 May . 38(8):2577-93."
COMMENT     Sequence Label: pPMQAK1
FEATURES             Location/Qualifiers
     source          1..8372
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     576..595
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     660..679
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             complement(join(823..1614,1615..1683))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1446..1465
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1684..1788)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1856..1874
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(1878..1921)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    1924..1945
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     CDS             complement(1974..2279)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(2076..2095)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     misc_feature    2621..2641
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     terminator      2642..2699
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(2723..2740)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      2881..3275
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
     oriT            3617..3704
                     /label=RSF1010 oriT
                     /note="origin of transfer of the broad-host-range plasmid 
                     RSF1010 (Scholz et al., 1989)"
     CDS             4943..5914
                     /codon_start=1
                     /gene="repB"
                     /product="replication protein B of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepB
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRRTALD
                     EYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
     CDS             6425..7264
                     /codon_start=1
                     /gene="repA"
                     /product="replication protein A of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepA
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
     CDS             7251..8102
                     /codon_start=1
                     /gene="repC"
                     /product="replication protein C of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepC
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
     CDS             complement(join(8225..8372,1..668))
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(join(8225..8372,1..668))
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
ORIGIN
        1 gcgattccga ctcgtccaac atcaatacaa cctattaatt tcccctcgtc aaaaataagg
       61 ttatcaagtg agaaatcacc atgagtgacg actgaatccg gtgagaatgg caaaagctta
      121 tgcatttctt tccagacttg ttcaacaggc cagccattac gctcgtcatc aaaatcactc
      181 gcatcaacca aaccgttatt cattcgtgat tgcgcctgag cgagacgaaa tacgcgatcg
      241 ctgttaaaag gacaattaca aacaggaatc gaatgcaacc ggcgcaggaa cactgccagc
      301 gcatcaacaa tattttcacc tgaatcagga tattcttcta atacctggaa tgctgttttc
      361 ccggggatcg cagtggtgag taaccatgca tcatcaggag tacggataaa atgcttgatg
      421 gtcggaagag gcataaattc cgtcagccag tttagtctga ccatctcatc tgtaacatca
      481 ttggcaacgc tacctttgcc atgtttcaga aacaactctg gcgcatcggg cttcccatac
      541 aatcgataga ttgtcgcacc tgattgcccg acattatcgc gagcccattt atacccatat
      601 aaatcagcat ccatgttgga atttaatcgc ggcctggagc aagacgtttc ccgttgaata
      661 tggctcataa caccccttgt attactgttt atgtaagcag acagttttat tgttcatgat
      721 gatatatttt tatcttgtgc aatgtaacat cagagatttt gagacacaac gtggctttgt
      781 tgaataaatc gaacttttgc tgagttgaag gatcagctcg agttaccaat gcttaatcag
      841 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
      901 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
      961 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     1021 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     1081 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     1141 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     1201 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     1261 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     1321 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     1381 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     1441 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     1501 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     1561 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     1621 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     1681 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     1741 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     1801 aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag
     1861 gcgtatcacg aggcagaatt tcagataaaa aaaatcctta gctttcgcta aggatgattt
     1921 ctggaattcg cggccgcttc tagagactgg ctgtgtataa gggagcctga catttatatt
     1981 ccccagaaca tcaggttaat ggcgtttttg atgtcatttt cgcggtggct gagatcagcc
     2041 acttcttccc cgataacgga gaccggcaca ctggccatat cggtggtcat catgcgccag
     2101 ctttcatccc cgatatgcac caccgggtaa agttcacggg agactttatc tgacagcaga
     2161 cgtgcactgg ccagggggat caccatccgt cgcccgggcg tgtcaataat atcactctgt
     2221 acatccacaa acagacgata acggctctct cttttatagg tgtaaacctt aaactgcatt
     2281 tcaccagccc ctgttctcgt cagcaaaaga gccgttcatt tcaataaacc gggcgacctc
     2341 agccatccct tcctgatttt ccgctttcca gcgttcggca cgcagacgac gggcttcatt
     2401 ctgcatggtt gcgcttacca gaccggagat attgacatca tatatgcctt gagcaactga
     2461 tagctgtcgc tgtcaactgt cactgtaata cgctgcttca tagcatacct ctttttgaca
     2521 tacttcgggt atacatatca gtatatattc ttataccgca aaaatcagcg cgcaaatacg
     2581 catactgtta tctggctttt agtaagccgg atccacgcgt tactagtagc ggccgctgca
     2641 gtccggcaaa aaagggcaag gtgtcaccac cctgcccttt ttctttaaaa ccgaaaagat
     2701 tacttcgcgt tatgcaggct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     2761 ggcgagcggt atcagctcac tcaaaggcgg taatcaattg agttctttta ccctcagccg
     2821 aaatgcctgc cgttgctaga cattgccagc cagtgcccgt cactcccgta ctaactgtca
     2881 cgaacccctg caataactgt cacgcccccc tgcaataact gtcacgaacc cctgcaataa
     2941 ctgtcacgcc cccaaacctg caaacccagc aggggcgggg gctggcgggg tgttggaaaa
     3001 atccatccat gattatctaa gaataatcca ctaggcgcgg ttatcagcgc ccttgtgggg
     3061 cgctgctgcc cttgcccaat atgcccggcc agaggccgga tagctggtct attcgctgcg
     3121 ctaggctaca caccgcccca ccgctgcgcg gcagggggaa aggcgggcaa agcccgctaa
     3181 accccacacc aaaccccgca gaaatacgct ggagcgcttt tagccgcttt agcggccttt
     3241 ccccctaccc gaagggtggg ggcgcgtgtg cagccccgca gggcctgtct cggtcgatca
     3301 ttcagcccgg ctcatccttc tggcgtggcg gcagaccgaa caaggcgcgg tcgtggtcgc
     3361 gttcaaggta cgcatccatt gccgccatga gccgatcctc cggccactcg ctgctgttca
     3421 ccttggccaa aatcatggcc cccaccagca ccttgcgcct tgtttcgttc ttgcgctctt
     3481 gctgctgttc ccttgcccgc acccgctgaa tttcggcatt gattcgcgct cgttgttctt
     3541 cgagcttggc cagccgatcc gccgccttgt tgctcccctt aaccatcttg acaccccatt
     3601 gttaatgtgc tgtctcgtag gctatcatgg aggcacagcg gcggcaatcc cgaccctact
     3661 ttgtagggga gggcgcactt accggtttct cttcgagaaa ctggcctaac ggccaccctt
     3721 cgggcggtgc gctctccgag ggccattgca tggagccgaa aagcaaaagc aacagcgagg
     3781 cagcatggcg atttatcacc ttacggcgaa aaccggcagc aggtcgggcg gccaatcggc
     3841 cagggccaag gccgactaca tccagcgcga aggcaagtat gcccgcgaca tggatgaagt
     3901 cttgcacgcc gaatccgggc acatgccgga gttcgtcgag cggcccgccg actactggga
     3961 tgctgccgac ctgtatgaac gcgccaatgg gcggctgttc aaggaggtcg aatttgccct
     4021 gccggtcgag ctgaccctcg accagcagaa ggcgctggcg tccgagttcg cccagcacct
     4081 gaccggtgcc gagcgcctgc cgtatacgct ggccatccat gccggtggcg gcgagaaccc
     4141 gcactgccac ctgatgatct ccgagcggat caatgacggc atcgagcggc ccgccgctca
     4201 gtggttcaag cggtacaacg gcaagacccc ggagaagggc ggggcacaga agaccgaagc
     4261 gctcaagccc aaggcatggc ttgagcagac ccgcgaggca tgggccgacc atgccaaccg
     4321 ggcattagag cgggctggcc acgacgcccg cattgaccac agaacacttg aggcgcaggg
     4381 catcgagcgc ctgcccggtg ttcacctggg gccgaacgtg gtggagatgg aaggccgggg
     4441 catccgcacc gaccgggcag acgtggccct gaacatcgac accgccaacg cccagatcat
     4501 cgacttacag gaataccggg aggcaataga ccatgaacgc aatcgacaga gtgaagaaat
     4561 ccagaggcat caacgagtta gcggagcaga tcgaaccgct ggcccagagc atggcgacac
     4621 tggccgacga agcccggcag gtcatgagcc agacccagca ggccagcgag gcgcaggcgg
     4681 cggagtggct gaaagcccag cgccagacag gggcggcatg ggtggagctg gccaaagagt
     4741 tgcgggaggt agccgccgag gtgagcagcg ccgcgcagag cgcccggagc gcgtcgcggg
     4801 ggtggcactg gaagctatgg ctaaccgtga tgctggcttc catgatgcct acggtggtgc
     4861 tgctgatcgc atcgttgctc ttgctcgacc tgacgccact gacaaccgag gacggctcga
     4921 tctggctgcg cttggtggcc cgatgaagaa cgacaggact ttgcaggcca taggccgaca
     4981 gctcaaggcc atgggctgtg agcgcttcga tatcggcgtc agggacgcca ccaccggcca
     5041 gatgatgaac cgggaatggt cagccgccga agtgctccag aacacgccat ggctcaagcg
     5101 gatgaatgcc cagggcaatg acgtgtatat caggcccgcc gagcaggagc ggcatggtct
     5161 ggtgctggtg gacgacctca gcgagtttga cctggatgac atgaaagccg agggccggga
     5221 gcctgccctg gtagtggaaa ccagcccgaa gaactatcag gcatgggtca aggtggccga
     5281 cgccgcaggc ggtgaacttc gggggcagat tgcccggacg ctggccagcg agtacgacgc
     5341 cgacccggcc agcgccgaca gccgccacta tggccgcttg gcgggcttca ccaaccgcaa
     5401 ggacaagcac accacccgcg ccggttatca gccgtgggtg ctgctgcgtg aatccaaggg
     5461 caagaccgcc accgctggcc cggcgctggt gcagcaggct ggccagcaga tcgagcaggc
     5521 ccagcggcag caggagaagg cccgcaggct ggccagcctc gaactgcccg agcggcagct
     5581 tagccgccac cggcgcacgg cgctggacga gtaccgcagc gagatggccg ggctggtcaa
     5641 gcgcttcggt gatgacctca gcaagtgcga ctttatcgcc gcgcagaagc tggccagccg
     5701 gggccgcagt gccgaggaaa tcggcaaggc catggccgag gccagcccag cgctggcaga
     5761 gcgcaagccc ggccacgaag cggattacat cgagcgcacc gtcagcaagg tcatgggtct
     5821 gcccagcgtc cagcttgcgc gggccgagct ggcacgggca ccggcacccc gccagcgagg
     5881 catggacagg ggcgggccag atttcagcat gtagtgcttg cgttggtact cacgcctgtt
     5941 atactatgag tactcacgca cagaaggggg ttttatggaa tacgaaaaaa gcgcttcagg
     6001 gtcggtctac ctgatcaaaa gtgacaaggg ctattggttg cccggtggct ttggttatac
     6061 gtcaaacaag gccgaggctg gccgcttttc agtcgctgat atggccagcc ttaaccttga
     6121 cggctgcacc ttgtccttgt tccgcgaaga caagcctttc ggccccggca agtttctcgg
     6181 tgactgatat gaaagaccaa aaggacaagc agaccggcga cctgctggcc agccctgacg
     6241 ctgtacgcca agcgcgatat gccgagcgca tgaaggccaa agggatgcgt cagcgcaagt
     6301 tctggctgac cgacgacgaa tacgaggcgc tgcgcgagtg cctggaagaa ctcagagcgg
     6361 cgcagggcgg gggtagtgac cccgccagcg cctaaccacc aactgcctgc aaaggaggca
     6421 atcaatggct acccataagc ctatcaatat tctggaggcg ttcgcagcag cgccgccacc
     6481 gctggactac gttttgccca acatggtggc cggtacggtc ggggcgctgg tgtcgcccgg
     6541 tggtgccggt aaatccatgc tggccctgca actggccgca cagattgcag gcgggccgga
     6601 tctgctggag gtgggcgaac tgcccaccgg cccggtgatc tacctgcccg ccgaagaccc
     6661 gcccaccgcc attcatcacc gcctgcacgc ccttggggcg cacctcagcg ccgaggaacg
     6721 gcaagccgtg gctgacggcc tgctgatcca gccgctgatc ggcagcctgc ccaacatcat
     6781 ggccccggag tggttcgacg gcctcaagcg cgccgccgag ggccgccgcc tgatggtgct
     6841 ggacacgctg cgccggttcc acatcgagga agaaaacgcc agcggcccca tggcccaggt
     6901 catcggtcgc atggaggcca tcgccgccga taccgggtgc tctatcgtgt tcctgcacca
     6961 tgccagcaag ggcgcggcca tgatgggcgc aggcgaccag cagcaggcca gccggggcag
     7021 ctcggtactg gtcgataaca tccgctggca gtcctacctg tcgagcatga ccagcgccga
     7081 ggccgaggaa tggggtgtgg acgacgacca gcgccggttc ttcgtccgct tcggtgtgag
     7141 caaggccaac tatggcgcac cgttcgctga tcggtggttc aggcggcatg acggcggggt
     7201 gctcaagccc gccgtgctgg agaggcagcg caagagcaag ggggtgcccc gtggtgaagc
     7261 ctaagaacaa gcacagcctc agccacgtcc ggcacgaccc ggcgcactgt ctggcccccg
     7321 gcctgttccg tgccctcaag cggggcgagc gcaagcgcag caagctggac gtgacgtatg
     7381 actacggcga cggcaagcgg atcgagttca gcggcccgga gccgctgggc gctgatgatc
     7441 tgcgcatcct gcaagggctg gtggccatgg ctgggcctaa tggcctagtg cttggcccgg
     7501 aacccaagac cgaaggcgga cggcagctcc ggctgttcct ggaacccaag tgggaggccg
     7561 tcaccgctga tgccatggtg gtcaaaggta gctatcgggc gctggcaaag gaaatcgggg
     7621 cagaggtcga tagtggtggg gcgctcaagc acatacagga ctgcatcgag cgcctttgga
     7681 aggtatccat catcgcccag aatggccgca agcggcaggg gtttcggctg ctgtcggagt
     7741 acgccagcga cgaggcggac gggcgcctgt acgtggccct gaaccccttg atcgcgcagg
     7801 ccgtcatggg tggcggccag catgtgcgca tcagcatgga cgaggtgcgg gcgctggaca
     7861 gcgaaaccgc ccgcctgctg caccagcggc tgtgtggctg gatcgacccc ggcaaaaccg
     7921 gcaaggcttc catagatacc ttgtgcggct atgtctggcc gtcagaggcc agtggttcga
     7981 ccatgcgcaa gcgccgccag cgggtgcgcg aggcgttgcc ggagctggtc gcgctgggct
     8041 ggacggtaac cgagttcgcg gcgggcaagt acgacatcac ccggcccaag gcggcaggct
     8101 gacccccccc actctattgt aaacaagaca tttttatctt ttatattcaa tggcttattt
     8161 tcctgctaat tggtaatacc atggtaccgc tctgccagtg ttacaaccaa ttaaccaatt
     8221 ctgattagaa aaactcatcg agcatcaaat gaaactgcaa tttattcata tcaggattat
     8281 caataccata tttttgaaaa agccgtttct gtaatgaagg agaaaactca ccgaggcagt
     8341 tccataggat ggcaagatcc tggtatcggt ct
//
