LOCUS       sequence-122291-        2476 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes a recombineering cassette for tagging a target gene within a
            genomic clone with a fluorescent protein. Cassette: bicistronic; 
            FRT-galK-FRT in 2nd mChO intron;use C-term after STOP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2476)
  AUTHORS   Tursun B, Cochella L, Carrera I, Hobert O
  TITLE     A toolkit and robust pipeline for the generation of fosmid-based 
            reporter genes in C. elegans.
  JOURNAL   PLoS One. 2009;4(3):e4625. doi: 10.1371/journal.pone.0004625. Epub 
            2009 Mar 4.
  PUBMED    19259264
REFERENCE   2  (bases 1 to 2476)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2009"; volume: "4(3):e4625. doi"; pages: " 
            10.1371/journal.pone.0004625. Epub 2009 Mar 4"
COMMENT     Sequence Label: pBALU12
FEATURES             Location/Qualifiers
     source          1..2476
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             255..275
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    complement(711..744)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     promoter        748..795
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     primer_bind     complement(1977..1993)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1977..1993)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     protein_bind    complement(2002..2035)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
ORIGIN
        1 gctgtctcat cctactttca cctagttaac tgcttgtctt aaaatctatg cttctcttta
       61 gtatctaaaa ttttcctaga agcttacaag tatataaatg gtctcttctc aataaaggtt
      121 gtatatttat tcatcttatt gaatctgcca tttcctcgtt tttgcgagtt tatatacctt
      181 ccaattttct ttctattgta ttttcaactt ctaattttaa ttcagggaaa ctgcttcaac
      241 gcatcatgac cgctccaaag aagaaacgca aagtaccggt agaaaaaatg gtctcaaagg
      301 gtgaagaaga taacatggca attattaaag agtttatgcg tttcaaggtg catatggagg
      361 gatctgtcaa tgggcatgag tttgaaattg aaggtgaagg agaaggccga ccatatgagg
      421 gaacacaaac cgcaaaacta aaggtaagtt taaacatata tatactaact aaccctgatt
      481 atttaaattt tcaggtaact aaaggcggac cattaccatt cgcctgggac atcctctctc
      541 cacagttcat gtatggaagt aaagcttatg ttaaacatcc ggcagatata ccagattatt
      601 tgaaactttc attcccggag ggttttaagt gggaacgcgt aatgaatttt gaagacggag
      661 gagttgttac agtgacgcaa gactcaaggt aagtttaaac agttcggtac gaagttccta
      721 tactttctag agaataggaa cttccctgtt gacaattaat catcggcata gtatatcggc
      781 atagtataat acgacaaggt gaggaactaa acccaggagg cagatcatga gtctgaaaga
      841 aaaaacacaa tctctgtttg ccaacgcatt tggctaccct gccactcaca ccattcaggc
      901 gcctggccgc gtgaatttga ttggtgaaca caccgactac aacgacggtt tcgttctgcc
      961 ctgcgcgatt gattatcaaa ccgtgatcag ttgtgcacca cgcgatgacc gtaaagttcg
     1021 cgtgatggca gccgattatg aaaatcagct cgacgagttt tccctcgatg cgcccattgt
     1081 cgcacatgaa aactatcaat gggctaacta cgttcgtggc gtggtgaaac atctgcaact
     1141 gcgtaacaac agcttcggcg gcgtggacat ggtgatcagc ggcaatgtgc cgcagggtgc
     1201 cgggttaagt tcttccgctt cactggaagt cgcggtcgga accgtattgc agcagcttta
     1261 tcatctgccg ctggacggcg cacaaatcgc gcttaacggt caggaagcag aaaaccagtt
     1321 tgtaggctgt aactgcggga tcatggatca gctaatttcc gcgctcggca agaaagatca
     1381 tgccttgctg atcgattgcc gctcactggg gaccaaagca gtttccatgc ccaaaggtgt
     1441 ggctgtcgtc atcatcaaca gtaacttcaa acgtaccctg gttggcagcg aatacaacac
     1501 ccgtcgtgaa cagtgcgaaa ccggtgcgcg tttcttccag cagccagccc tgcgtgatgt
     1561 caccattgaa gagttcaacg ctgttgcgca tgaactggac ccgatcgtgg caaaacgcgt
     1621 gcgtcatata ctgactgaaa acgcccgcac cgttgaagct gccagcgcgc tggagcaagg
     1681 cgacctgaaa cgtatgggcg agttgatggc ggagtctcat gcctctatgc gcgatgattt
     1741 cgaaatcacc gtgccgcaaa ttgacactct ggtagaaatc gtcaaagctg tgattggcga
     1801 caaaggtggc gtacgcatga ccggcggcgg atttggcggc tgtatcgtcg cgctgatccc
     1861 ggaagagctg gtgcctgccg tacagcaagc tgtcgctgaa caatatgaag caaaaacagg
     1921 tattaaagag actttttacg tttgtaaacc atcacaagga gcaggacagt gctgaggatc
     1981 cactagttct agagcggccg cgaagttcct atactttcta gagaatagga acttctaact
     2041 aaccatacat atttaaattt tcagcctcca agatggagaa tttatttata aagtcaaact
     2101 tcgaggaacg aatttcccct cggatggacc tgttatgcag aagaagacta tgggatggga
     2161 agcttcaagt gaaagaatgt accctgaaga cggtgctctt aagggagaga ttaaacaacg
     2221 tcttaaattg aaagatggag gacattacga tgctgaggta agtttaaaca tgattttact
     2281 aactaactaa tctgatttaa attttcaggt gaagacaact tacaaagcca aaaaaccagt
     2341 tcagctgcca ggagcgtaca atgttaatat taaactggat atcacctccc acaacgagga
     2401 ttacactatc gttgagcaat atgaaagagc tgaagggcgg cactcgacag gtggcatgga
     2461 tgaattgtat aagtaa
//
