LOCUS       sequence-122310-       10787 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses R1335K mutant SpCas9 fused to TALE-TS3 in mammalian cell.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10787)
  AUTHORS   Bolukbasi MF, Gupta A, Oikemus S, Derr AG, Garber M, Brodsky MH, Zhu
            LJ, Wolfe SA
  TITLE     DNA-binding-domain fusions enhance the targeting range and precision
            of Cas9.
  JOURNAL   Nat Methods. 2015 Oct 19. doi: 10.1038/nmeth.3624.
  PUBMED    26480473
REFERENCE   2  (bases 1 to 10787)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Oct 19. doi: 10.1038/nmeth.3624."
COMMENT     Sequence Label: pCSDest2-2XNLS-SpCas9-MT3-NLS-3XHA-NLS-TALE_TS3
FEATURES             Location/Qualifiers
     source          1..10787
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        37..55
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     37..54
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     protein_bind    110..134
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      138..147
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             159..185
                     /codon_start=1
                     /product="nuclear localization signal (Makkerh et al., 
                     1996)"
                     /label=NLS
                     /translation="PAAKKKKLD"
     CDS             249..275
                     /codon_start=1
                     /product="nuclear localization signal (Makkerh et al., 
                     1996)"
                     /label=NLS
                     /translation="PAAKKKKLD"
     CDS             297..4397
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKKYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             4410..4430
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4431..4520
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     CDS             4533..4559
                     /codon_start=1
                     /product="nuclear localization signal (Makkerh et al., 
                     1996)"
                     /label=NLS
                     /translation="PAAKKKKLD"
     polyA_signal    6838..6972
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6862..6881)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6916..6935
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(7085..7105)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(7085..7103)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7124..7140)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7124..7140)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7137..7159)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7148..7164
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7172..7178,7179..7196,7197..7202))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7217..7238
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7355..7372)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7526..8114)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7606..7625)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8285..9076,9077..9145))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8908..8927
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9146..9250)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(9276..9731)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9413..9434)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9625..9644
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        join(9805..10787,1..2)
                     /label=CMV IE94 promoter
                     /note="enhancer/promoter region of simian cytomegalovirus 
                     major immediate early transcription unit IE94"
ORIGIN
        1 accgccattc tgcctgggga cgtcggagca agcttgattt aggtgacact atagaataca
       61 agctacttgt tctttttgca ggatcccatc gattcgaatt caaggatcaa caagtttgta
      121 caaaaaagca ggctggcgcc accatgggta gagccgcgcc ggcagctaag aaaaagaaac
      181 tggatcaatt gggcgctagc ggtagtcccg ggtctggagg tagcggctca agtggccgta
      241 cggcagctcc tgcggcaaaa aagaaaaagt tggactctga attcggaagc ggaagcgaca
      301 agaagtactc cattgggctc gatatcggta ccaacagcgt cggctgggcc gtcattacgg
      361 acgagtacaa ggtgccgagc aaaaaattca aagttctggg caataccgat cgccacagca
      421 taaagaagaa cctcattgga gccctcctgt tcgactccgg ggagacggcc gaagccacgc
      481 ggctcaaaag aacagcacgg cgcagatata cccgcagaaa gaatcggatc tgctacctgc
      541 aggagatctt tagtaatgag atggctaagg tggatgactc tttcttccat aggctggagg
      601 agtccttttt ggtggaggag gataaaaagc acgagcgcca cccaatcttt ggcaatatcg
      661 tggacgaggt ggcgtaccat gaaaagtacc caaccatata tcatctgagg aagaagctgg
      721 tagacagtac tgataaggct gacttgcggt tgatctatct cgcgctggcg cacatgatca
      781 aatttcgggg acacttcctc atcgaggggg acctgaaccc agacaacagc gatgtcgaca
      841 aactctttat ccaactggtt cagacttaca atcagctttt cgaggagaac ccgatcaacg
      901 catccggcgt tgacgccaaa gcaatcctga gcgctaggct gtccaaatcc cggcggctcg
      961 aaaacctcat cgcacagctc cctggggaga agaagaacgg cctgtttggt aatcttatcg
     1021 ccctgtcact cgggctgacc cccaacttta aatctaactt cgacctggcc gaagatgcca
     1081 agctgcaact gagcaaagac acctacgatg atgatctcga caatctgctg gcccagatcg
     1141 gcgaccagta cgcagacctt tttttggcgg caaagaacct gtcagacgcc attctgctga
     1201 gtgatattct gcgagtgaac acggagatca ccaaagctcc gctgagcgct agtatgatca
     1261 agcgctatga tgagcaccac caagacttga ctttgctgaa ggcccttgtc agacagcaac
     1321 tgcctgagaa gtacaaggaa attttcttcg atcagtctaa aaatggctac gccggataca
     1381 ttgacggcgg agcaagccag gaggaatttt acaaatttat taagcccatc ttggaaaaaa
     1441 tggacggcac cgaggagctg ctggtaaagc tgaacagaga agatctgttg cgcaaacagc
     1501 gcactttcga caatggaagc atcccccacc agattcacct gggcgaactg cacgctatcc
     1561 tcaggcggca agaggatttc tacccctttt tgaaagataa cagggaaaag attgagaaaa
     1621 tcctcacatt tcggataccc tactatgtag gccccctcgc tcggggaaat tccagattcg
     1681 cgtggatgac tcgcaaatca gaagagacca tcactccctg gaacttcgag gaagtcgtgg
     1741 ataagggggc ctctgcccag tccttcatcg aaaggatgac taactttgat aaaaatctgc
     1801 ctaacgaaaa ggtgcttcct aaacactctc tgctgtacga gtacttcaca gtttataacg
     1861 agctcaccaa ggtcaaatac gtcacagaag ggatgagaaa gccagcattc ctgtctggag
     1921 agcagaagaa agctatcgtg gacctcctct tcaagacgaa ccggaaagtt accgtgaaac
     1981 agctcaaaga agactatttc aaaaagattg aatgtttcga ctctgttgaa atcagcggag
     2041 tggaggatcg cttcaacgca tccctgggaa cgtatcacga tctcctgaaa atcattaaag
     2101 acaaggactt cctggacaat gaggagaacg aggacattct tgaggacatt gtcctcaccc
     2161 ttacgttgtt tgaagatagg gagatgattg aagaacgctt gaaaacttac gctcatctct
     2221 tcgacgacaa agtcatgaaa cagctcaaga gacgccgata tacaggatgg gggcggctgt
     2281 caagaaaact gatcaatggc atccgagaca agcagagtgg aaagacaatc ctggattttc
     2341 ttaagtccga tggatttgcc aaccggaact tcatgcagtt gatccatgat gactctctca
     2401 cctttaagga ggacatccag aaagcacaag tttctggcca gggggacagt cttcacgagc
     2461 acatcgctaa tcttgcaggt agcccagcta tcaaaaaggg aatactgcag accgttaagg
     2521 tcgtggatga actcgtcaaa gtaatgggaa ggcataagcc cgagaatatc gttatcgaga
     2581 tggcccgaga gaaccaaact acccagaagg gacagaagaa cagtagggaa aggatgaaga
     2641 ggattgaaga gggtataaaa gaactggggt cccaaatcct taaggaacac ccagttgaaa
     2701 acacccagct tcagaatgag aagctctacc tgtactacct gcagaacggc agggacatgt
     2761 acgtggatca ggaactggac atcaaccggt tgtccgacta cgacgtggat catatcgtgc
     2821 cccaaagctt tctcaaagat gattctattg ataataaagt gttgacaaga tccgataaaa
     2881 atagagggaa gagtgataac gtcccctcag aagaagttgt caagaaaatg aaaaattatt
     2941 ggcggcagct gctgaacgcc aaactgatca cacaacggaa gttcgataat ctgactaagg
     3001 ctgaacgagg tggcctgtct gagttggata aagccggctt catcaaaagg cagcttgttg
     3061 agacacgcca gatcaccaag cacgtggccc aaattctcga ttcacgcatg aacaccaagt
     3121 acgatgaaaa tgacaaactg attcgagagg tgaaagttat tactctgaag tctaagctgg
     3181 tctcagattt cagaaaggac tttcagtttt ataaggtgag agagatcaac aattaccacc
     3241 atgcgcatga tgcctacctg aatgcagtgg taggcactgc acttatcaaa aaatatccca
     3301 agctggaatc tgaatttgtt tacggagact ataaagtgta cgatgttagg aaaatgatcg
     3361 caaagtctga gcaggaaata ggcaaggcca ccgctaagta cttcttttac agcaatatta
     3421 tgaatttttt caagaccgag attacactgg ccaatggaga gattcggaag cgaccactta
     3481 tcgaaacaaa cggagaaaca ggagaaatcg tgtgggacaa gggtagggat ttcgcgacag
     3541 tccgcaaggt cctgtccatg ccgcaggtga acatcgttaa aaagaccgaa gtacagaccg
     3601 gaggcttctc caaggaaagt atcctcccga aaaggaacag cgacaagctg atcgcacgca
     3661 aaaaagattg ggaccccaag aaatacggcg gattcgattc tcctacagtc gcttacagtg
     3721 tactggttgt ggccaaagtg gagaaaggga agtctaaaaa actcaaaagc gtcaaggaac
     3781 tgctgggcat cacaatcatg gagcgatcca gcttcgagaa aaaccccatc gactttctcg
     3841 aagcgaaagg atataaagag gtcaaaaaag acctcatcat taagctgccc aagtactctc
     3901 tctttgagct tgaaaacggc cggaaacgaa tgctcgctag tgcgggcgag ctgcagaaag
     3961 gtaacgagct ggcactgccc tctaaatacg ttaatttctt gtatctggcc agccactatg
     4021 aaaagctcaa agggtctccc gaagataatg agcagaagca gctgttcgtg gaacaacaca
     4081 aacactacct tgatgagatc atcgagcaaa taagcgagtt ctccaaaaga gtgatcctcg
     4141 ccgacgctaa cctcgataag gtgctttctg cttacaataa gcacagggat aagcccatca
     4201 gggagcaggc agaaaacatt atccacttgt ttactctgac caacttgggc gcgcctgcag
     4261 ccttcaagta cttcgacacc accatagaca gaaagaagta cacctctaca aaggaggtcc
     4321 tggacgccac actgattcat cagtcaatta cggggctcta tgaaacaaga atcgacctct
     4381 ctcagctcgg tggagacggc accggcgggc ccaagaagaa gaggaaggta tacccatacg
     4441 atgttcctga ctatgcgggc tatccctatg acgtcccgga ctatgcagga tcgtatcctt
     4501 atgacgttcc agattacgct ggatccgccg ctccggcagc taagaaaaag aaactggatt
     4561 tcgaatccgg agtacctatg gtggacttga ggacactcgg ttattcgcaa cagcaacagg
     4621 agaaaatcaa gcctaaggtc aggagcaccg tcgcgcaaca ccacgaggcg cttgtggggc
     4681 atggcttcac tcatgcgcat attgtcgcgc tttcacagca ccctgcggcg cttgggacgg
     4741 tggctgtcaa ataccaagat atgattgcgg ccctgcccga agccacgcac gaggcaattg
     4801 taggggtcgg taaacagtgg tcgggagcgc gagcacttga ggcgctgctg actgtggcgg
     4861 gtgagcttag ggggcctccg ctccagctcg acaccgggca gctgctgaag atcgcgaaga
     4921 gagggggagt aacagcggta gaggcagtgc acgcctggcg caatgcgctc accggggccc
     4981 ccttgcccct gaacctgacc ccggaccaag tggtggctat cgccagcaac ggtggcggca
     5041 agcaagcgct cgaaacggtg cagcggctgt tgccggtgct gtgccaggac catggcctga
     5101 ccccggacca agtggtggct atcgccagca acaatggcgg caagcaagcg ctcgaaacgg
     5161 tgcagcggct gttgccggtg ctgtgccagg accatggcct gaccccggac caagtggtgg
     5221 ctatcgccag caacaatggc ggcaagcaag cgctcgaaac ggtgcagcgg ctgttgccgg
     5281 tgctgtgcca ggaccatggc ctgaccccgg accaagtggt ggctatcgcc agcaacattg
     5341 gcggcaagca agcgctcgaa acggtgcagc ggctgttgcc ggtgctgtgc caggaccatg
     5401 gcctgacccc ggaccaagtg gtggctatcg ccagcaacaa tggcggcaag caagcgctcg
     5461 aaacggtgca gcggctgttg ccggtgctgt gccaggacca tggcctgact ccggaccaag
     5521 tggtggctat cgccagccac gatggcggca agcaagcgct cgaaacggtg cagcggctgt
     5581 tgccggtgct gtgccaggac catggcctga ccccggacca agtggtggct atcgccagca
     5641 acaatggcgg caagcaagcg ctcgaaacgg tgcagcggct gttgccggtg ctgtgccagg
     5701 accatggcct gaccccggac caagtggtgg ctatcgccag caacaatggc ggcaagcaag
     5761 cgctcgaaac ggtgcagcgg ctgttgccgg tgctgtgcca ggaccatggc ctgaccccgg
     5821 accaagtggt ggctatcgcc agcaacaatg gcggcaagca agcgctcgaa acggtgcagc
     5881 ggctgttgcc ggtgctgtgc caggaccatg gcctgacccc ggaccaagtg gtggctatcg
     5941 ccagcaacaa tggcggcaag caagcgctcg aaacggtgca gcggctgttg ccggtgctgt
     6001 gccaggacca tggcctgacc ccggaccaag tggtggctat cgccagcaac attggcggca
     6061 agcaagcgct cgaaacggtg cagcggctgt tgccggtgct gtgccaggac catggcctga
     6121 ccccggacca agtggtggct atcgccagca acaatggcgg caagcaagcg ctcgaaacgg
     6181 tgcagcggct gttgccggtg ctgtgccagg accatggcct gaccccggac caagtggtgg
     6241 ctatcgccag caacattggc ggcaagcaag cgctcgaaac ggtgcagcgg ctgttgccgg
     6301 tgctgtgcca ggaccatggc ctgaccccgg accaagtggt ggctatcgcc agcaacattg
     6361 gcggcaagca agcgctcgaa acggtgcagc ggctgttgcc ggtgctgtgc caggaccatg
     6421 gcctgacccc ggaccaagtg gtggctatcg ccagcaacaa tggcggcaag caagcgctcg
     6481 aaacggtgca gcggctgttg ccggtgctgt gccaggacca tggcctgacc ccggaccaag
     6541 tggtggctat cgctaataat aacggaggac ggccagcctt ggagtccatc gtagcccaat
     6601 tgtccaggcc cgatcccgcg ttggctgcgt taacgaatga ccatctggtg gcgttggcat
     6661 gtcttggtgg acgacccgcg ctcgatgcag tcaaaaaggg tctgcctcat gctcccgcat
     6721 tgatcaaaag aaccaaccgg cggattcccg agagaacttc ccatcgagtc gcgggatctt
     6781 aatccggata agcggccgcc tcgagatatc tagaactata gtgagtcgta ttacgtagat
     6841 ccagacatga taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa
     6901 aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc
     6961 aataaacaag ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggaggtg
     7021 tgggaggttt tttaattcgc ggccgcggcg ccaatgcatt gggcccggta cccagctttt
     7081 gttcccttta gtgagggtta attgcgcgct tggcgtaatc atggtcatag ctgtttcctg
     7141 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
     7201 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
     7261 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
     7321 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     7381 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     7441 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     7501 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     7561 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     7621 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     7681 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     7741 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     7801 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     7861 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     7921 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta
     7981 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     8041 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     8101 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     8161 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     8221 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     8281 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     8341 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     8401 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     8461 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     8521 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     8581 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     8641 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     8701 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     8761 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     8821 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     8881 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     8941 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     9001 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     9061 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     9121 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     9181 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     9241 gggttccgcg cacatttccc cgaaaagtgc cacctaaatt gtaagcgtta atattttgtt
     9301 aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg
     9361 caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg
     9421 gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta
     9481 tcagggcgat ggcccactac gtgaaccatc accctaatca agttttttgg ggtcgaggtg
     9541 ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa
     9601 gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct
     9661 ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct
     9721 acagggcgcg tcccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg
     9781 ggcctcttcg ctattacgcc agtcgaccat agccaattca atatggcgta tatggactca
     9841 tgccaattca atatggtgga tctggacctg tgccaattca atatggcgta tatggactcg
     9901 tgccaattca atatggtgga tctggacccc agccaattca atatggcgga cttggcacca
     9961 tgccaattca atatggcgga cttggcactg tgccaactgg ggaggggtct acttggcacg
    10021 gtgccaagtt tgaggagggg tcttggccct gtgccaagtc cgccatattg aattggcatg
    10081 gtgccaataa tggcggccat attggctata tgccaggatc aatatatagg caatatccaa
    10141 tatggcccta tgccaatatg gctattggcc aggttcaata ctatgtattg gccctatgcc
    10201 atatagtatt ccatatatgg gttttcctat tgacgtagat agcccctccc aatgggcggt
    10261 cccatatacc atatatgggg cttcctaata ccgcccatag ccactccccc attgacgtca
    10321 atggtctcta tatatggtct ttcctattga cgtcatatgg gcggtcctat tgacgtatat
    10381 ggcgcctccc ccattgacgt caattacggt aaatggcccg cctggctcaa tgcccattga
    10441 cgtcaatagg accacccacc attgacgtca atgggatggc tcattgccca ttcatatccg
    10501 ttctcacgcc ccctattgac gtcaatgacg gtaaatggcc cacttggcag tacatcaata
    10561 tctattaata gtaacttggc aagtacatta ctattggaag gacgccaggg tacattggca
    10621 gtactcccat tgacgtcaat ggcggtaaat ggcccgcgat ggctgccaag tacatcccca
    10681 ttgacgtcaa tggggagggg caatgacgca aatgggcgtt ccattgacgt aaatgggcgg
    10741 taggcgtgcc taatgggagg tctatataag caatgctcgt ttaggga
//
