LOCUS       sequence-122399-         850 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  nuclease competent SP-Cas9 fused to VPR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 850)
  AUTHORS   Kiani S, Chavez A, Tuttle M, Hall RN, Chari R, Ter-Ovanesyan D, Qian
            J, Pruitt BW, Beal J, Vora S, Buchthal J, Kowal EJ, Ebrahimkhani MR,
            Collins JJ, Weiss R, Church G
  TITLE     Cas9 gRNA engineering for genome editing, activation and repression.
  JOURNAL   Nat Methods. 2015 Sep 7. doi: 10.1038/nmeth.3580.
  PUBMED    26344044
REFERENCE   2  (bases 1 to 850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Sep 7. doi: 10.1038/nmeth.3580."
COMMENT     Sequence Label: hCas9-VPR
FEATURES             Location/Qualifiers
     source          1..850
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     2..26
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      114..123
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gagctcgttt agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc
       61 atagaagaca ccgggaccga tccagcctcc ggactctaga ggatcgaacc cttgccacca
      121 tggacaagaa gtactccatt gggctcgata tcggcacaaa cagcgtcggc tgggccgtca
      181 ttacggacga gtacaaggtg ccgagcaaaa aattcaaagt tctgggcaat accgatcgcc
      241 acagcataaa gaagaacctc attggcgccc tcctgttcga ctccggggag acggccgaag
      301 ccacgcggct caaaagaaca gcacggcgca gatatacccg cagaaagaat cggatctgct
      361 acctgcagga gatctttagt aatgagatgg ctaaggtgga tgactctttc ttccataggc
      421 tggaggagtc ctttttggtg gaggaggata aaaagcacga gcgccaccca atctttggca
      481 atatcgtgga cgaggtggcg taccatgaaa agtacccaac catatatcat ctgaggaaga
      541 agcttgtaga cagtactgat aaggctgact tgcggttgat ctatctcgcg ctggcgcata
      601 tgatcaaatt tcggggacac ttcctcatcg agggggacct gaacccagac aacagcgatg
      661 tcgacaaact ctttatccaa ctggttcaga cttacaatca gcttttcgaa gagaacccga
      721 tcaacgcatc cggagttgac gccaaagcaa tcctgagcgc taggctgtcc aaatcccggc
      781 ggctcgaaaa cctcatcgca cagctccctg gggagaagaa gaacggcctg tttggtaatc
      841 ttatcgccct
//
