LOCUS       sequence-122410-         833 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  storage vector for BASIC part: Kan.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 833)
  AUTHORS   Storch M, Casini A, Mackrow B, Fleming T, Trewhitt H, Ellis T, 
            Baldwin GS
  TITLE     BASIC: A New Biopart Assembly Standard for Idempotent Cloning 
            Provides Accurate, Single-Tier DNA Assembly for Synthetic Biology.
  JOURNAL   ACS Synth Biol. 2015 Apr 16.
  PUBMED    25746445
REFERENCE   2  (bases 1 to 833)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2015 Apr 16."
COMMENT     Sequence Label: BASIC_3_Kan
FEATURES             Location/Qualifiers
     source          1..833
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(606..612,613..630,631..636))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    651..672
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(792..809)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agacgtttcc cgttgaatat ggctcataac accacttgta ttactgttta tgtaagcaga
       61 cagttttatt gttcatgatg atatattttt atcttgtgca atgtaacatc agagattttg
      121 agacacaacg tggctttgtt gaataaatcg aacggacaga gacccaccag aatctttcta
      181 gaagatctcc tacaatattc tcagctgcca tggaaaatcg atgttcttct tttattctct
      241 caagattttc aggctgtata ttaaaactta tattaagaac tatgctaacc acctcatcag
      301 gaaccgttgt aggtggcgtg ggttttcttg gcaatcgact ctcatgaaaa ctacgagcta
      361 aatattcaat atgttcctct tgaccaactt tattctgcat tttttttgaa cgaggtttag
      421 agcaagcttc aggaaactga gacaggaatt ttattaaaaa tttaaatttt gaagaaagtt
      481 cagggttaat agcatccatt ttttgctttg caagttcctc agcattctta acaaaagacg
      541 tctcttttga catgtttaaa gtttaaacct cctgtgtgaa attattatcc gctcataatt
      601 ccacacatta tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
      661 taactcacat taattgcgtt gcgctcactg ccaattgctt tccagtcggg aaacctgtcg
      721 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
      781 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcg
//
