LOCUS       sequence-122637-         939 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  FRB conjugated plasma membrane targeting sequences from GAP43 
            (MLCCMRRTKQVEKNDDQKI) .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 939)
  AUTHORS   Varnai P, Thyagarajan B, Rohacs T, Balla T
  TITLE     Rapidly inducible changes in phosphatidylinositol 4,5-bisphosphate 
            levels influence multiple regulatory functions of the lipid in 
            intact living cells.
  JOURNAL   J Cell Biol. 2006 Nov 6;175(3):377-82.
  PUBMED    17088424
REFERENCE   2  (bases 1 to 939)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2006-11-6"; volume: "175(3)"; pages: "377-82"
COMMENT     Sequence Label: PM-FRB-CFP
FEATURES             Location/Qualifiers
     source          1..939
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(129..146)
                     /codon_start=1
                     /product="HQHQHQ metal affinity tag"
                     /label=HQ tag
                     /note="The HQ tag binds to nickel and other metal ions much
                     like the 6xHis tag, but may allow elution at lower 
                     concentrations of imidazole (Godat et al., 2008)."
                     /translation="HQHQHQ"
     CDS             161..439
                     /codon_start=1
                     /product="FKBP-rapamycin binding domain of human FRAP"
                     /label=FRB
                     /translation="ILWHEMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTL
                     KETSFNQAYGRDLMEAQEWCRKYMKSGNVKDLTQAWDLYYHVFRRISK"
     CDS             446..472
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(530..551)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(791..810)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 agcagagctg gtttagtgaa ccgtcagatc cgctagcacc atgctgtgct gtatgagaag
       61 aaccaaacag gttgaaaaaa atgatgacga ccaaaagatt gatcccacca gatctgcgaa
      121 ttcaggtgct ggtgctggtg ctggtgctat cctatctaga atcctctggc atgagatgtg
      181 gcatgaaggc ctggaagagg catctcgttt gtactttggg gaaaggaacg tgaaaggcat
      241 gtttgaggtg ctggagccct tgcatgctat gatggaacgg ggcccccaga ctctgaagga
      301 aacatccttt aatcaggcct atggtcgaga tttaatggag gcccaagagt ggtgcaggaa
      361 gtacatgaaa tcagggaatg tcaaggacct cacccaagcc tgggacctct attatcatgt
      421 gttccgacga atctcaaaga ctagttatcc gtacgacgta ccagactacg caccggtcgc
      481 caccctggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct
      541 ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac
      601 ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc
      661 caccctcgtg accaccctga cctggggcgt gcagtgcttc agccgctacc ccgaccacat
      721 gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat
      781 cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac
      841 cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg
      901 gcacaagctg gagtacaact acatcagcca caacgtcta
//
