LOCUS       sequence-122668-         904 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  donor plasmid for targeting PAX6 locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 904)
  AUTHORS   Chen Y, Cao J, Xiong M, Petersen AJ, Dong Y, Tao Y, Huang CT, Du Z, 
            Zhang SC
  TITLE     Engineering Human Stem Cell Lines with Inducible Gene Knockout using
            CRISPR/Cas9.
  JOURNAL   Cell Stem Cell. 2015 Jul 1. pii: S1934-5909(15)00261-1. doi: 
            10.1016/j.stem.2015.06.001.
  PUBMED    26145478
REFERENCE   2  (bases 1 to 904)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2015 Jul 1. pii: S1934-5909(15)00261-1. doi: 
            10.1016/j.stem.2015.06.001."
COMMENT     Sequence Label: PAX6 CKO 2/7 Donor plasmid
FEATURES             Location/Qualifiers
     source          1..904
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        8..26
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 agctcgaaat taaccctcac taaagggaac aaaagctggt acccacgctc accccctcct
       61 gttcgtactt tttaaagcag tgaggcgagg tagacagggt gtgtcacagt acagttaaag
      121 gggtgaagat ctaaacgcca aaagagaagt taatcacaat aagtgaggtt tgggataaaa
      181 agttgggctt gcccctttca aagtcccaga aagctgggag gtagatggag agggggccat
      241 tgggaagttt ttttggtgta gggagaggag tagaagataa agggtaagca gagtgttggg
      301 ttctgggggt cttgtgaagt tccttaagga aggagggagt gtggccctgc agccctccca
      361 aactgctcca gcctatgctc tccggcacca ggaagttcca aggttccctt cccctggtct
      421 ccaaacttca ggtattcctc tcccctcaca ccccttcaac ctcagctctt ggcctctact
      481 ccttactcca ctgttcctcc tgtttccccc ttcccctttt cctggttctt tatatttttg
      541 caaagtggga tccgaacttg ctagattttc caattctccc aagccagacc agagcagcct
      601 cttttaaagg atggagactt ctgtggcaga tgccgctgaa aatgtgggtg taatgctggg
      661 acttagagtt tgatgacagt ttgactgagc cctagatgca tgtgtttttc ctgagagtga
      721 ggctcagaga gcccatggac gtatgctgtt gaaccacagc ttgatatacc tttttctcct
      781 tctgttttgt cttaggggga agactttaac taggggcgcg cagatgtgtg aggcctttta
      841 ttgtgagagt ggacagacat ccgagatttc aggcaagttc tgtggtggct gctttgggct
      901 caaa
//
