LOCUS       sequence-122669-         440 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  donor plasmid for targeting PAX6 locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 440)
  AUTHORS   Chen Y, Cao J, Xiong M, Petersen AJ, Dong Y, Tao Y, Huang CT, Du Z, 
            Zhang SC
  TITLE     Engineering Human Stem Cell Lines with Inducible Gene Knockout using
            CRISPR/Cas9.
  JOURNAL   Cell Stem Cell. 2015 Jul 1. pii: S1934-5909(15)00261-1. doi: 
            10.1016/j.stem.2015.06.001.
  PUBMED    26145478
REFERENCE   2  (bases 1 to 440)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2015 Jul 1. pii: S1934-5909(15)00261-1. doi: 
            10.1016/j.stem.2015.06.001."
COMMENT     Sequence Label: PAX6 CKO 2/7 Donor plasmid
FEATURES             Location/Qualifiers
     source          1..440
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(68..101)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(132..165)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
ORIGIN
        1 cctacccggt agaatttcga ggtcgagatc ctaagcttgg ctggacgtaa actcctcttc
       61 agacctaata acttcgtata gcatacatta tacgaagtta tattaagggt tattgaatat
      121 gatcggaatt cgaagttcct atactttcta gagaatagga acttccctgc ttctccagta
      181 tcgagaagag ccaagcaaac gccctcccct cccgggctgc cgggcgcgga gcggggagca
      241 gccgccgcag gtccccgggc ctcagtcggt cggcggccgg gccagcgcgg gcgtcgcgag
      301 tccctgtgtc ctcccctgac cctcgggtcc gcgcaccccg agcccgaagt cccagaaaga
      361 ccagaggcac ttactgttct gcatgctggc tctggctggg ggccgcggga ttccacgggg
      421 ctcgaatatg gggctctgtt
//
