LOCUS       sequence-122697-        1040 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  TCP4 expression in yeast.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1040)
  AUTHORS   Rubio-Somoza I, Zhou CM, Confraria A, Martinho C, von Born P, 
            Baena-Gonzalez E, Wang JW, Weigel D
  TITLE     Temporal control of leaf complexity by miRNA-regulated licensing of 
            protein complexes.
  JOURNAL   Curr Biol. 2014 Nov 17;24(22):2714-9. doi: 
            10.1016/j.cub.2014.09.058. Epub 2014 Nov 6.
  PUBMED    25448000
REFERENCE   2  (bases 1 to 1040)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cub.2014.09.058"; journalName: "Curr Biol"; date: 
            "2014-11-17- 17"; volume: "24"; issue: "22"; pages: "2714-9"
COMMENT     Sequence Label: JW630
FEATURES             Location/Qualifiers
     source          1..1040
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     45..64
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        45..63
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      70..79
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             82..108
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 ttcgatgatg aagatacccc accaaaccca aaaaaagaga tctttaatac gactcactat
       61 agggcgagcg ccgccatgga gtacccatac gacgtaccag attacgctca tatggccatg
      121 gaggccagtg aattccaccc gggtgggcat cgatacggga tccatatgtc tgacgaccaa
      181 ttccatcacc cgccgcctcc ttcttcaatg aggcaccgtt ctacgtcgga tgcggcggac
      241 ggcggctgcg gcgagattgt tgaggtgcaa ggtggtcaca ttgttcggtc taccggaaga
      301 aaagaccgcc acagcaaagt ctgcacggct aaagggccac gtgaccggcg cgtgagactc
      361 tctgctcaca cggcgattca gttttacgat gttcaagaca ggcttggttt cgaccgacct
      421 agcaaagccg ttgattggct tatcaaaaag gctaagactt ccattgacga gctcgctgag
      481 cttcctccct ggaatcccgc cgatgcaatt cgcctagccg ctgctaacgc taaacccaga
      541 agaaccaccg ccaaaaccca aatctctccg tctccgccac cgccgcaaca gcaacaacaa
      601 caacaacagc ttcagttcgg tgttggcttc aacggaggag gagcagagca tccgagtaac
      661 aacgagtcga gttttctccc gccgtcaatg gattcagatt cgatagctga cactataaag
      721 tcgttttttc cggtgattgg ctcttcaacg gaggctcctt cgaatcataa ccttatgcac
      781 aactatcatc atcagcatcc gccggatttg ctttctcgaa ctaatagcca aaaccaagat
      841 ctccgtctct cgctgcaatc gttcccggat ggtccaccgt cgcttctgca ccaccaacat
      901 caccaccaca cctctgcttc cgcctccgag cctactctgt tctacggaca gagcaatccg
      961 ttagggtttg acacatcgag ttgggagcag cagtcgtcgg aattcggaag gattcagaga
     1021 ctagtggctt ggaacagcgg
//
