LOCUS       sequence-122736-        4457 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of green fluorescent genetically encoded Ca2+ 
            indicator for optical imaging (version 1.2).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4457)
  AUTHORS   Zhao Y, Araki S, Wu J, Teramoto T, Chang YF, Nakano M, Abdelfattah 
            AS, Fujiwara M, Ishihara T, Nagai T, Campbell RE
  TITLE     An Expanded Palette of Genetically Encoded Ca2+ Indicators.
  JOURNAL   Science. 2011 Sep 30;333(6051):1888-91. doi: 10.1126/science.1208592
  PUBMED    21903779
REFERENCE   2  (bases 1 to 4457)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2011-09-30- 30"; volume: "333"; 
            issue: "6051"; pages: "1888-91"
COMMENT     Sequence Label: CMV-G-GECO1.2
FEATURES             Location/Qualifiers
     source          1..4457
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    42..266
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     255..272
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     promoter        292..310
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     292..309
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             complement(371..814)
                     /codon_start=1
                     /product="calcium-binding protein"
                     /label=calmodulin
                     /translation="DQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEA
                     ELQDMINEVDADGDGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGYIGAAEL
                     RHVMTNLGEKLTDEEVDEMIRVADIDGDGQVNYEEFVQMMTAK"
     primer_bind     complement(1322..1343)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             complement(1556..1612)
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKTGHAVRAIGRLSS"
     primer_bind     complement(1661..1680)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1662..1680)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(1725..1928)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(1754..1774)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        1929..2308
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     2480..2499
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        2574..2678
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2679..2747,2748..3539)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2897..2916)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3710..4298
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4199..4218
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttccggacta gagccccagc tggttctttc cgcctcagaa gccatagagc ccaccgcatc
       61 cccagcatgc ctgctattgt cttcccaatc ctcccccttg ctgtcctgcc ccaccccacc
      121 ccccagaata gaatgacacc tactcagaca atgcgatgca atttcctcat tttattagga
      181 aaggacagtg ggagtggcac cttccagggt caaggaaggc acgggggagg ggcaaacaac
      241 agatggctgg caactagaag gcacagtcga ggctgatcag cgagctctag catttaggtg
      301 acactataga atagggccct ctagatgcat gctcgagcgg ccgccagtgt gatggatatc
      361 tgcagaattc ttacttcgct gtcatcattt gtacaaactc ttcgtagttt acctgaccat
      421 ccccatcgat gtctgctacc ctgatcattt catcaacctc ttcatctgtt aacttctctc
      481 caaggtttgt catcacgtgg cgaagctctg ctgcgccgat gtagccattg ccgtccttat
      541 caaacacacg gaacgcttct ctaatttctt cttcactgtc tgtgtctttc atttttcttg
      601 ccatcattgt caggaactca gggaagtcga ttgtgccgtc accgtcggca tctacttcat
      661 tgatcatgtc ctgcagctct gcttctgtgg ggttctgccc cagagaccgc atcaccgtcc
      721 ccagctcctt ggttgttatc gtcccatccc cgtccttgtc aaatagggag aaagcctctt
      781 taaattctgc gatctgctct tcagtcagtt ggtcacgcgt gttgtactcc agcttgtgcc
      841 ccaggatgtt gccgtcctcc ttgaagtcga tgcccttcag ctcgatgcgg ttcaccaggg
      901 tgtcgccctc gaacttcacc tcggcgcggg tcttgtagtt gccgtcgccc ttgaagaaga
      961 tggtgcgctc ctggatgtag ccttcgggca tggcggactt gaagaagtcg tgctgcttca
     1021 tgtggtcggg gtagcggctg aagcactgca cgccgtaggt cagggtggtc acgagggtgg
     1081 gccagggcac gggcagcttg ccggtggtgc agatgaactt cagggtcagc ttgccgtagg
     1141 tggcatcgcc ctcaccctcg ccggacacgc tgaacttgtg gccgtttacg tcgccgtcca
     1201 gctcgacctg gatgggcacc accccggtga acagctcctc gcccttgctc accatggatt
     1261 cgctccctcc ggtaccgccc ttgtacagct cgtccatgcc gagagtgatc ccggcggcgg
     1321 tcacgaactc cagcaggacc atgtgatcgc gcttctcgtt ggggtctttc gaaagcatgg
     1381 actgcacgct caggtagtgg ttgtcgggca gcagcacggg gccgtcgccg atgggggtgt
     1441 tctgctggta gtggtaggcg agctgcacgc cgccgccctc gatgttgtgg cggatcttga
     1501 agtacgcctt gatgccgttc ttctgctcgt cggccttgat atagacgttc tcgagtgagc
     1561 tcagccgacc tatagctctg actgcgtgac ctgtcttatt ccacttacga cgtgatgagt
     1621 cgaccatggt ggatccgagc tcggtaccaa gcttgggtct ccctatagtg agtcgtatta
     1681 atttcgataa gccagtaagc agtgggttct ctagttagcc agagagctct gcttatatag
     1741 acctcccacc gtacacgcct accgcccatt tgcgtcaatg gggcggagtt gttacgacat
     1801 tttggaaagt cccgttgatt ttggtgccaa aacaaactcc cattgacgtc aatggggtgg
     1861 agacttggaa atccccgtga gtcaaaccgc tatccacgcc cattgatgta ctgccaaaac
     1921 cgcatcacca tggtaatagc gatgactaat acgtagatgt actgccaagt aggaaagtcc
     1981 cataaggtca tgtactgggc ataatgccag gcgggccatt taccgtcatt gacgtcaata
     2041 gggggcgtac ttggcatatg atacacttga tgtactgcca agtgggcagt ttaccgtaaa
     2101 tactccaccc attgacgtca atggaaagtc cctattggcg ttactatggg aacatacgtc
     2161 attattgacg tcaatgggcg ggggtcgttg ggcggtcagc caggcgggcc atttaccgta
     2221 agttatgtaa cgcggaactc catatatggg ctatgaacta atgaccccgt aattgattac
     2281 tattaataac tagtcaataa tcaatgtcaa cgcgtatatc tggcccgtac atcgcgaagc
     2341 agcgcaaaac gcctaaccct aagcagattc ttcatgcaat tgtcggtcaa gccttgcctt
     2401 gttgtagctt aaattttgct cgcgcactac tcagcgacct ccaacacaca agcagggagc
     2461 agatactggc ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatagggga
     2521 tcgggagatc tcccgatccg tcgacgtcag gtggcacttt tcggggaaat gtgcgcggaa
     2581 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     2641 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     2701 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     2761 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     2821 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     2881 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     2941 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     3001 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     3061 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     3121 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     3181 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     3241 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     3301 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     3361 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     3421 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     3481 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     3541 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     3601 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     3661 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     3721 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     3781 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     3841 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     3901 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     3961 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     4021 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     4081 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     4141 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     4201 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     4261 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     4321 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     4381 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     4441 cgaccgagcg cagcgag
//
