LOCUS       sequence-122779-       12355 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lgg-1 tagged with oxCerulean-oxVenus (dFP, or double fluorescent 
            protein), expressed in body wall muscle, for monitoring autophagy.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12355)
  AUTHORS   Chapin HA, Okada M, Merz AJ, Miller DL
  TITLE     Tissue-specific autophagy responses to aging and stress in C. 
            elegans.
  JOURNAL   Aging (Albany NY). 2015 Jun 30.
  PUBMED    26142908
REFERENCE   2  (bases 1 to 12355)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Aging 
            (Albany NY). 2015 Jun 30."
COMMENT     Sequence Label: pMyo-3::oxCerulean-oxVenus::Lgg-1
FEATURES             Location/Qualifiers
     source          1..12355
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(214..233)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     333..355
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(393..411)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        479..583
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(584..652,653..1444)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(802..821)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1615..2203
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2104..2123
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(3839..3850)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    5916..5936
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    8442..8466
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             9297..9317
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     protein_bind    complement(10069..10093)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(10823..10843)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(10851..10868)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(10851..10867)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 cacgtgacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca
       61 tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca
      121 gttgcgcagc ctgaatggcg aatggcgcct gatgcggtat tttctcctta cgcatctgtg
      181 cggtatttca caccgcatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
      241 aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc
      301 ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc
      361 accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt
      421 taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg
      481 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
      541 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
      601 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
      661 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
      721 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
      781 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
      841 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
      901 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
      961 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
     1021 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     1081 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
     1141 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
     1201 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     1261 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
     1321 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     1381 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     1441 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
     1501 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     1561 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     1621 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     1681 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     1741 agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa
     1801 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     1861 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     1921 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     1981 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     2041 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     2101 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     2161 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     2221 ctttttacgg ttcctggtct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     2281 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgatacca gcggataaca
     2341 atttcacagg gccctgtcgg attatgggct cttcttttat cctctacttt ctttctagaa
     2401 aatgcaaatg ttgagtcaga aattgctgaa tggagtacaa ctgcaatggg tttagcttat
     2461 gtttctggaa caattactgg tggaattgtg attgaaaagt aaggttatat ttcatttcaa
     2521 taaatgttac cacagtataa caaaaacaag ttacattgtt cagagtatgt ctgaatgtat
     2581 tattattatt gtatttgtgc atcagtactg tagttgtact gaagaagggt ttaaaataat
     2641 ctcaacgttt ttaagacgag ttagtgagaa tacggtcgca gttaggatgt ttggacatag
     2701 ttttattaga taatcgtgaa ctaactcact ataaaaaccc gctaactcat tgaataagat
     2761 agttttaaac gttcaaaatt tgaagacaaa attgtatgaa taagtttaca gagttggtcg
     2821 acgcaagctt ctccttctct tcacatttct caataattta gctctaattg cattcgtatt
     2881 cttcgcaaaa atacgtattc tgattgaccc gatgaaatac ggatgcctag ttgcattgat
     2941 catctacggt tatacttatg ggtgaggttt ttcaaatata ttttgattta atcgaataat
     3001 gaaatttcag aactggagtt ggtccaattt cgtggtttat atcctctgaa ctggtacctc
     3061 aaaaacatcg tagtattgct caatctgtgg catattcaat gaatactgta atggtggtta
     3121 tcagtacatt tacagtatta cctctatatt ccgttattgg tagttatgct ttccttattc
     3181 tatattcaat tccttcattt ataagtatgc ttatattatt tcgctactta ccggaaacca
     3241 aaggacgaga gattcatgat attgtgaatg aacttaaaaa aaaatgaaaa cctttgaaaa
     3301 cgttcatacg gccgaaattt tacttgatcg agccaattga acttttgtta agacttacag
     3361 aaaagtgaga gattttgtta caaaaaagtg agaaaaaagc gaaaatgaac aggaaaaaga
     3421 agaaaaatac acccgaagta tcatataaaa attaaaaaga ttgtttgacc tggcggaact
     3481 tttttgcaaa aactctgaat atccatggca catgcttcgt gcaaaacagg atccaaatga
     3541 atatcaacaa gagattcttg aagacgacga gccacttgct cagagcattg cttatctcga
     3601 atgagaccct tgttaaaatc atttttcaaa cattatatga cattgtcgaa atgtcctcct
     3661 gattccatga tggtagcaaa ctcacttcgt ctttgagcca atttatccaa gtccttgtaa
     3721 aaagtattcg aaaattgtta acggataaaa tgtttattat aatatcaaaa acaatttgtc
     3781 agttgaccac tttttgatat atttgacaga aaccgggatg aattggctca aagttagggc
     3841 gcccttctat tacagggttt ctgataacaa accggttatt aactcccaac aagggatgat
     3901 ttcaattcat catgctcaaa ttgacccaaa ttaagttaca tgacaaattc atcgcccttt
     3961 tcaactcttc ttggctcatc atctgttatt ctgttcctat tctgtctgca ccctataccc
     4021 ctttgcatac tctctcgtca ttcctttctt ggatagtgtc ttcttctccc cagctctgct
     4081 acttctatga cttgcgcccg ctgcttttcc gctcgttcct ctcctctctg acgtcttctt
     4141 ctgcttcttt tctccttcct ttttcccttc tcaatcttcc tctttttccc atttctgtca
     4201 atcattcgaa gaagaagaag aagaagaccc tcattcattt catttttttt tctgtcggtg
     4261 tgtgctgctc ggttaagagt gagctctcta ttccacgtct tcttcttttt cttcttcgat
     4321 tcgaatcaat cactccacca aaacgcattc gtttttggga ttcaccccgc ggttcgcaac
     4381 aggtttcttt ttcaaattat tagcgttata aatagaaaaa tggtggagtt tcaataaaaa
     4441 tgataattta caaagtgtat tttgattaca tgtactcaaa aaggttgaaa tttttcatac
     4501 cagttttccg gaaatccatc tgatatcatt atcgtatttt tctttttaaa aatgttttca
     4561 aaaaaaaaca aaatatagct ggttatttgg caccctctaa ttaccatttt cctgtcacac
     4621 cacactcttt tccttcttcc acttctttcc gttttcagcc gcttccaacc aaaccgatat
     4681 gaaagccgag caacaacaat cgattccacc cggctcggcg accttcccgt cgcaggtgag
     4741 actcagaaaa ctagagaaac cgctcaacta actcttgata tccgatttca ttcttttctc
     4801 ttttcttttt gttgaacttt tccatatttc cagatgccac ggccaccacc aagcaccgaa
     4861 caaggaatca caacggaatc ggagcttgcg aagaaagctc aaatcactcc gaacgacgtt
     4921 ttagcacttc cgggaatcac tcaaggtatc ttgctccctt tggatttcag tttaaaacat
     4981 aaatttagga ttcttatgct ccccatctgc gaacatctat aacatcgagt tcaccaagtt
     5041 ccaaatccgt gatctggaca ctgagcaagt gcttttcgag atcgccaaac cggagaacga
     5101 tcaggagaat gatgagtcgc cacaggagtc ggcaagatac gtgcgttata gatttgctcc
     5161 aaactttttg aaactcaaaa cggtcggagc aactgtggaa ttcaaagtag gagacatccc
     5221 aatccatcat ttccgaatga tcgaacgtca cttcttcaaa gatcgccttc tgaagtgttt
     5281 tgactttgaa ttcggattct gtattccgaa ttcacgaaac aactgtgaac atatctatga
     5341 gttccctcaa ctctctcaac aactcagtga gtcattattc taaaaagtac aattcaaaag
     5401 actaatctct ttttcagtgg acgacatgat caacaatcca aacgagactc gttctgacag
     5461 cttctatttc gtcgataaca aactcgtcat gcacaacaaa gccgactact catatgatgc
     5521 ataaatattt aatacaaaaa tgttctggat aattattctg tcgaatagaa aaaaaactcc
     5581 aaatgtgatt aaattccaat aattcctgtc tagtttgttc cttccccttc cccttctcat
     5641 gttcaatgca ttcctaagct tttcagttcc ccccttgttt ctatattttt tcgctgtcct
     5701 gtcacactcg ctaaaaacac taatcacacg gaaatctgtt ttcaataaaa actccaactt
     5761 taactcattt tcaatttcaa ctgaaagatt ttttcattag agaatgtcta gaactaggcc
     5821 cgggctacgt aatacgactc acttaaggcc ttgactagag ggtaccagag ctcacctagg
     5881 caggaacagc tatgaccatg attacgccaa gctatcaact ttgtatagaa aagttgagtt
     5941 gagtgattat agtctctgtt ttcgttaatt ttgaattttg cttgataagg ctgcaacaaa
     6001 gatcaggttg acatatattt tcagtaattt attttaacct gtactctatc actgccggct
     6061 ataataagtt cttgaataaa ataattttcc cgacaaaaca tgagtatttc tttcgaaaat
     6121 aaaagtgcag gctaattaga gattattctg taattaactg cataatttgt cacgtgccat
     6181 agttttacat tccactacgt catagttctt aaaatactaa tctcctgaaa atagaagtag
     6241 gtgaagaaag tttaattatc agttctaaaa tgacaattga tctttggaat atgttctgaa
     6301 actaccgatc attgaacaga tgctatttga atgatataga attgtatatt tgcaatttct
     6361 gaaacgcgtt cttaaaggca cacagattaa ttcaaaaggg tctggccgca aaaaggttta
     6421 tggtggccga ttttgagttt tgtgtgtgat tgctttttca caatcagtgt tttcaggatt
     6481 atgtgatgaa ctagatcttc aagtttcgtt acatttcata tgttttcgga actcacgaag
     6541 tacatattgg gtattgtgct caaaaaattc agcaatcagc ttcgctccgc tgactttaga
     6601 acccaaaaaa atagtatggc caaactgact gtgttacgat catttcaatt tttcaataca
     6661 tatttaagat ttctaagagt aagaaggtca aaaactgttc tggaatacat atatattttt
     6721 caggttacaa ttagtcaaaa agtgcactga aatatacgtt ttaatttcac gaataaccca
     6781 attagttcaa tgtatttttg gtcaaccaac gttaaagttt ggcttccaac caattatcat
     6841 ttctgatcaa ccacaatgtt ttttctttat ctgcaagtta attttttatt tttatccaga
     6901 tgtttggcat atttttcaat tcttcactag cgcccacttc ttgcacttcc ggcgccctga
     6961 atctaatgca tctgttgcaa gaattgaaag accaatcaac acattgtttt cttcacgaga
     7021 tactgaagaa aatgaataaa aacagagaaa aagagccatg tgattagtga caactgttgc
     7081 taacagatac catagcttgg acttggtacg tgatggcaac gtatgggtca acaaaaatga
     7141 ttgcagaggg ggtgcaaaac agtcaagtcg agaaaatatg aaaaacagaa aacaaagaac
     7201 agaaaaatgg gtttgagagt cagtataatt tataaaagaa aaattgtaca tagaaattaa
     7261 ccatttttgt agaagaagtt atttttcaag catcgttaaa aattattcaa agcaccttat
     7321 ttcatattta attttaaaca tggttaaatg aacaacacgg tgcgcaatca ggaaaacttg
     7381 aaatctgaaa ctgttgttgt gatcttcttc gcaactgttc agatagcact agtgtaatgt
     7441 taagagtgcg cgaatataat ggaatataat ggatcacacc tcctgccatc aggtaaacgt
     7501 ctctgttatc acatatttcc aactattaaa tttttacctt ttacagtttt acattttttt
     7561 gaaaaaagta actttttgtc ttcaaaatcc ctgacgaaaa tatcaaatat tttaatcgag
     7621 actgcagagg aaccgattga tgatttggaa aatccagctt tacctgtgta agaactgaaa
     7681 agtttcataa ccctagggta ttcccagtta cattccccac tggctaacaa tagcacccag
     7741 tttttcatca cctttcttca aatttctcgg cgatttgtta aaaacaaaat ttgtgtccct
     7801 tctctgatat ctctatgtct ctaaacacaa gttcatcgga aaacgaagga gggtaggtgt
     7861 tggttgggct cccgaagtga aaatagaaga gcaagaatag aatattagag agagagtgca
     7921 gagagggcgg gatagctccc gggattccgt tttcttcttc tttatcttca acgatgatgt
     7981 gtgtgcgtgt tgtatagatt ctgttgctcc cccacaactc gctccgaagg ctcaatacaa
     8041 ttcaattgat attggaggag agcctaccgg agtgggagga taagaagaaa cataagaaga
     8101 agaagaagaa gaagcatgct tctggttttt gatgctatga aaacggcaca aaaagatgat
     8161 tgaggtccct tttcaatacc ttctctcatc tttcaaatcc cattgaaacc taaaacttct
     8221 caccacgctt taccattgtt ctccaaaaac ttatagcaat gtctataact tttttatctc
     8281 tgaaaagcag tgttccattt ttctttttcc tattttattt caattgtttc tcacatttcg
     8341 tttggattct ttgcttgtca accagcttct tcttccactt ttaccgtcta attttcaggg
     8401 cagggagcca tcaaacccac gaccactaga tccatctaga aacaagtttg tacaaaaaag
     8461 caggctcaat ggtgtcaaag ggcgaagagc tgtttactgg agtcgtccca attctggtgg
     8521 agctggatgg agatgtcaac gggcataagt tttcagtgcg aggagaggga gaaggagacg
     8581 ctaccaacgg caagctgaca ctgaaattca tcagcaccac agggaagctg cccgtgcctt
     8641 gggtaagttt aaacatatat atactaacta accctgatta tttaaatttt cagccaaccc
     8701 tggtcactac cctgagttgg ggcgtccagt catttgcccg atacccagac cacatgaagc
     8761 agcatgattt ctttaaatcc gctatgcccg agggctacgt gcaggaacgg acaattttct
     8821 ttaaggacga tggaacatac aaaactagta agtttaaaca gttcggtact aactaaccat
     8881 acatatttaa attttcagga gcagaggtga agttcgaagg agacactctg gtcaacagga
     8941 tcgagctgaa gggcattgac tttaaagaag atggaaatat cctgggccac aaactggagt
     9001 acaacgccat ccatggcaac gtgtacatta cagctgataa gcagaaaaac gggattaagg
     9061 caaatttcgg actgaactgc aatgtggaag acgggagtgt ccagctggcc gatcactacc
     9121 agcagaacac tcctatcggc gacgggcccg tgctgctgcc tgataatcat tatctgagca
     9181 cccagtccgt cctgtctaag gaccccaatg agaaaagaga tcacatggtg ctgctggagt
     9241 ttgtgaccgc tgctggcatt accctgggga tggacgagct gtacaaggga ccaggcgaga
     9301 atttgtattt tcagggtcgt cttgtcaagt tccttgttgg aggaccaggc atggtgtcta
     9361 aaggcgagga actgttcact ggggtggtgc ccatcctggt ggagctggac ggcgatgtga
     9421 atgggcacaa gtttagcgtg cgcggagagg gagaagggga cgctaccaac ggaaagctga
     9481 cactgaaact gatttccacc acaggcaagc tgcccgtgcc ttggccaaca ctggtgacta
     9541 ccctgggata cggcctccag agcttcgccc ggtatccaga ccacatgaag cagcatgatt
     9601 tctttaaatc agctatgccc gagggctacg tgcaggaacg gacaatcttc tttaaggacg
     9661 atgggacata taaaactaga gccgaggtga agtttgaagg ggacactctg gtgaacagga
     9721 tcgagctgaa gggaattgac ttcaaagaag atggaaatat cctgggccac aagctggagt
     9781 acaacttcaa cagccataac gtgtatatca ccgccgataa gcagaaaaac ggcatcaagg
     9841 ctaatttcaa aattaggcac aatgtggaag acggcggggt gcagctggct gatcattacc
     9901 agcagaacac acctatcggg gacggacccg tgctgctgcc tgataatcac tacctgagct
     9961 atcagtccgt gctgtctaag gacccaaacg agaaacggga tcatatggtg ctgaaagaat
    10021 ttgtgactgc cgctgggatt accctgggaa tggatgagct gtataagcac ccagctttct
    10081 tgtacaaagt gggaatgaag tgggcttaca aggaggagaa caactttgag aagcgtcgtg
    10141 ccgaaggaga caagatccgc agaaagtacc cagaccgtat tccagtgatt gttgagaaag
    10201 caccaaagtc aaagctccat gacttggata agaagaagta cttggtccca tccgatctta
    10261 ctgttggaca gttctacttc ctcatcagaa aacgcatcca acttcgtcca gaagatgctc
    10321 tgttcttctt tgtcaacaat gtcattccac aaaccatgac cacaatggga caactctacc
    10381 aggtaactaa ccagtcgttt attttcattt aattaaccct tttctttatt acaggaccat
    10441 cacgaggaag acttgttcct ttacatcgcc tacagtgacg aaagtgtgta tggaggagag
    10501 gtcgaaaaga aggaataaag tgtcatgtat tatccgccga cgaatgtgta tacttgtaca
    10561 atggaaattt catacttcta atggaaaccc aaagcccctt taaaaaaatc ttctcatttt
    10621 ctgtaataat tctgtatttc cttcacattt tcttctcatt tttctcgtat gacggttccg
    10681 agtagtgact tctattcaaa ctattgtcta cgaagttgct cttctcccct cccagaaaat
    10741 catgttttct ctcggatttt gatatttttt tgctattact ttcaaatttt tgattcatga
    10801 ataaacgaag aagacacaaa accaacttta ttatacatag ttgataattc actggccgtc
    10861 gttttacact cgagacgtac ggtgcgcgcg atgcattcga agatctgccc actagtgagt
    10921 cgtattataa gtgcaagtaa gatcagtgtt tgtttcgata tcagtctgtt tcgtaacggt
    10981 cttctgtata actacaaaaa agaataaaaa accgtatcac aactgagaaa aaagacaaaa
    11041 cttattgaac agtgttcctt tcaaaaaatt attttgtaaa aaatcgagtt tttctccaaa
    11101 ataacgcacg caatcaaaaa caagaaaact aacgtgttct cctgggcgtg gaactcctta
    11161 tcaagattac cattattgtc tgtcagaatt gatatttcaa ttatttcata tctagacaaa
    11221 cccgggtgtc ttgcatttat tagtactgtg agtttacata caaacgaggc ttactcagat
    11281 aacatagagt cacctctaat tagcaaaaac ccatttctaa ttagctattt cgaccagttc
    11341 taaagtcacc gtaattagaa atggtttttg tagcttttct aattagaaat gaccccgaga
    11401 taagtgaaat atggcatttt ctgggttttc tagtaagaaa tgatcccgca cgtagtgatt
    11461 ccgcgttaca agccaaattc aaattaaaaa agtatattac acaattcaag ttacattatt
    11521 ttcaggaaac aatggcgaag agcaaagcag agattcaaaa ggaggaaaga gacaacagat
    11581 atgaacgaag acttcttgaa aagagaaaga gacatgaggt gagtaaccat gataataaat
    11641 aatcaagtaa gggctataga tttataaatt ctggagttaa cagtttcgaa aaaaagtagt
    11701 tttcatatgt gcgaaggaat atcaaaaata ggggaatagc ctaaacgagt gagaaaatta
    11761 aatgtttgtt attttttcaa aaatgtctgc agggccgtta tcctttcaaa tgcactctat
    11821 caaacataga tcatatccat catatcttat ccagaattta tatcatatcc aagattgaga
    11881 gcgtttcaag gaaaatctca aacaaccata caattttgta gatcgacaac aaggcctcca
    11941 cttcttcatc gatggcctct gcagcacctg caccaccaag tttgtatttt tcatattaat
    12001 ataaagtgag ccacgcatag tcgtttttag gatttttgcc agatcgatgt cgcgcaaaca
    12061 gactgttttt ttttctttga aagccgtgcg tgacatgggc cacggattgt aacgccgcgc
    12121 tggatggata aaaatttatt cagtttttta gcctttacaa aatatggttc gactactttc
    12181 tttattttta catttttata ggtgctcatt gagctgagat attttgggta taatcggaaa
    12241 atcgctgatt ttggtcattt ttctgcacta aagattacac aaaaagttca gtatttagat
    12301 tcattgttga aaattgggga tgatctacag atacatatcg ccgtgttcaa aaaca
//
