LOCUS       sequence-122817-       10278 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Ubiquitously expressed mitochondrial Tomm-7 tagged wtih 
            oxCerulean-oxVenus (dFP, or double fluorescent protein) for 
            monitoring mitophagy..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10278)
  AUTHORS   Chapin HA, Okada M, Merz AJ, Miller DL
  TITLE     Tissue-specific autophagy responses to aging and stress in C. 
            elegans.
  JOURNAL   Aging (Albany NY). 2015 Jun 30.
  PUBMED    26142908
REFERENCE   2  (bases 1 to 10278)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Aging 
            (Albany NY). 2015 Jun 30."
COMMENT     Sequence Label: pEft-3::Cerulean-Venus::Tomm-7
FEATURES             Location/Qualifiers
     source          1..10278
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(214..233)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     333..355
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(393..411)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        479..583
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(584..652,653..1444)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(802..821)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1615..2203
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2104..2123
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(3839..3850)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    5916..5936
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        5937..6536
                     /gene="eft-3"
                     /label=eft-3 promoter
                     /note="promoter for the C. elegans elongation factor 
                     1-alpha gene (Mitrovich and Anderson, 2000)"
     protein_bind    6545..6569
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             7400..7420
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     protein_bind    complement(8172..8196)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(8746..8766)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(8774..8791)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8774..8790)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 cacgtgacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca
       61 tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca
      121 gttgcgcagc ctgaatggcg aatggcgcct gatgcggtat tttctcctta cgcatctgtg
      181 cggtatttca caccgcatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
      241 aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc
      301 ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc
      361 accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt
      421 taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg
      481 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
      541 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
      601 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
      661 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
      721 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
      781 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
      841 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
      901 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
      961 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
     1021 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     1081 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
     1141 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
     1201 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     1261 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
     1321 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     1381 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     1441 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
     1501 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     1561 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     1621 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     1681 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     1741 agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa
     1801 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     1861 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     1921 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     1981 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     2041 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     2101 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     2161 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     2221 ctttttacgg ttcctggtct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     2281 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgatacca gcggataaca
     2341 atttcacagg gccctgtcgg attatgggct cttcttttat cctctacttt ctttctagaa
     2401 aatgcaaatg ttgagtcaga aattgctgaa tggagtacaa ctgcaatggg tttagcttat
     2461 gtttctggaa caattactgg tggaattgtg attgaaaagt aaggttatat ttcatttcaa
     2521 taaatgttac cacagtataa caaaaacaag ttacattgtt cagagtatgt ctgaatgtat
     2581 tattattatt gtatttgtgc atcagtactg tagttgtact gaagaagggt ttaaaataat
     2641 ctcaacgttt ttaagacgag ttagtgagaa tacggtcgca gttaggatgt ttggacatag
     2701 ttttattaga taatcgtgaa ctaactcact ataaaaaccc gctaactcat tgaataagat
     2761 agttttaaac gttcaaaatt tgaagacaaa attgtatgaa taagtttaca gagttggtcg
     2821 acgcaagctt ctccttctct tcacatttct caataattta gctctaattg cattcgtatt
     2881 cttcgcaaaa atacgtattc tgattgaccc gatgaaatac ggatgcctag ttgcattgat
     2941 catctacggt tatacttatg ggtgaggttt ttcaaatata ttttgattta atcgaataat
     3001 gaaatttcag aactggagtt ggtccaattt cgtggtttat atcctctgaa ctggtacctc
     3061 aaaaacatcg tagtattgct caatctgtgg catattcaat gaatactgta atggtggtta
     3121 tcagtacatt tacagtatta cctctatatt ccgttattgg tagttatgct ttccttattc
     3181 tatattcaat tccttcattt ataagtatgc ttatattatt tcgctactta ccggaaacca
     3241 aaggacgaga gattcatgat attgtgaatg aacttaaaaa aaaatgaaaa cctttgaaaa
     3301 cgttcatacg gccgaaattt tacttgatcg agccaattga acttttgtta agacttacag
     3361 aaaagtgaga gattttgtta caaaaaagtg agaaaaaagc gaaaatgaac aggaaaaaga
     3421 agaaaaatac acccgaagta tcatataaaa attaaaaaga ttgtttgacc tggcggaact
     3481 tttttgcaaa aactctgaat atccatggca catgcttcgt gcaaaacagg atccaaatga
     3541 atatcaacaa gagattcttg aagacgacga gccacttgct cagagcattg cttatctcga
     3601 atgagaccct tgttaaaatc atttttcaaa cattatatga cattgtcgaa atgtcctcct
     3661 gattccatga tggtagcaaa ctcacttcgt ctttgagcca atttatccaa gtccttgtaa
     3721 aaagtattcg aaaattgtta acggataaaa tgtttattat aatatcaaaa acaatttgtc
     3781 agttgaccac tttttgatat atttgacaga aaccgggatg aattggctca aagttagggc
     3841 gcccttctat tacagggttt ctgataacaa accggttatt aactcccaac aagggatgat
     3901 ttcaattcat catgctcaaa ttgacccaaa ttaagttaca tgacaaattc atcgcccttt
     3961 tcaactcttc ttggctcatc atctgttatt ctgttcctat tctgtctgca ccctataccc
     4021 ctttgcatac tctctcgtca ttcctttctt ggatagtgtc ttcttctccc cagctctgct
     4081 acttctatga cttgcgcccg ctgcttttcc gctcgttcct ctcctctctg acgtcttctt
     4141 ctgcttcttt tctccttcct ttttcccttc tcaatcttcc tctttttccc atttctgtca
     4201 atcattcgaa gaagaagaag aagaagaccc tcattcattt catttttttt tctgtcggtg
     4261 tgtgctgctc ggttaagagt gagctctcta ttccacgtct tcttcttttt cttcttcgat
     4321 tcgaatcaat cactccacca aaacgcattc gtttttggga ttcaccccgc ggttcgcaac
     4381 aggtttcttt ttcaaattat tagcgttata aatagaaaaa tggtggagtt tcaataaaaa
     4441 tgataattta caaagtgtat tttgattaca tgtactcaaa aaggttgaaa tttttcatac
     4501 cagttttccg gaaatccatc tgatatcatt atcgtatttt tctttttaaa aatgttttca
     4561 aaaaaaaaca aaatatagct ggttatttgg caccctctaa ttaccatttt cctgtcacac
     4621 cacactcttt tccttcttcc acttctttcc gttttcagcc gcttccaacc aaaccgatat
     4681 gaaagccgag caacaacaat cgattccacc cggctcggcg accttcccgt cgcaggtgag
     4741 actcagaaaa ctagagaaac cgctcaacta actcttgata tccgatttca ttcttttctc
     4801 ttttcttttt gttgaacttt tccatatttc cagatgccac ggccaccacc aagcaccgaa
     4861 caaggaatca caacggaatc ggagcttgcg aagaaagctc aaatcactcc gaacgacgtt
     4921 ttagcacttc cgggaatcac tcaaggtatc ttgctccctt tggatttcag tttaaaacat
     4981 aaatttagga ttcttatgct ccccatctgc gaacatctat aacatcgagt tcaccaagtt
     5041 ccaaatccgt gatctggaca ctgagcaagt gcttttcgag atcgccaaac cggagaacga
     5101 tcaggagaat gatgagtcgc cacaggagtc ggcaagatac gtgcgttata gatttgctcc
     5161 aaactttttg aaactcaaaa cggtcggagc aactgtggaa ttcaaagtag gagacatccc
     5221 aatccatcat ttccgaatga tcgaacgtca cttcttcaaa gatcgccttc tgaagtgttt
     5281 tgactttgaa ttcggattct gtattccgaa ttcacgaaac aactgtgaac atatctatga
     5341 gttccctcaa ctctctcaac aactcagtga gtcattattc taaaaagtac aattcaaaag
     5401 actaatctct ttttcagtgg acgacatgat caacaatcca aacgagactc gttctgacag
     5461 cttctatttc gtcgataaca aactcgtcat gcacaacaaa gccgactact catatgatgc
     5521 ataaatattt aatacaaaaa tgttctggat aattattctg tcgaatagaa aaaaaactcc
     5581 aaatgtgatt aaattccaat aattcctgtc tagtttgttc cttccccttc cccttctcat
     5641 gttcaatgca ttcctaagct tttcagttcc ccccttgttt ctatattttt tcgctgtcct
     5701 gtcacactcg ctaaaaacac taatcacacg gaaatctgtt ttcaataaaa actccaactt
     5761 taactcattt tcaatttcaa ctgaaagatt ttttcattag agaatgtcta gaactaggcc
     5821 cgggctacgt aatacgactc acttaaggcc ttgactagag ggtaccagag ctcacctagg
     5881 caggaacagc tatgaccatg attacgccaa gctatcaact ttgtatagaa aagttggcac
     5941 ctttggtctt ttattgtcaa cttccattgg ttcttccatt gtttctgtta aattaatgaa
     6001 tttttcataa aataaagaca ttatacaata taaaaatgaa gaatttattg aaaataaact
     6061 gccagagaga aaaagtatgc aacactcccg ccgagagtgt ttgaaatggt gtacggtaca
     6121 ttttcgtgct aggagttaga tgtgcaggca gcaacgagag ggggagagat ttttttgggc
     6181 cttgtgaaat taacgtgagt tttctggtca tctgactaat catgttggtt ttttgttggt
     6241 ttattttgtt tttatctttg tttttatcca gattaggaaa tttaaatttt atgaatttat
     6301 aatgaggtca aacattcagt cccagcgttt ttcctgttct cactgtttag tcgaattttt
     6361 attttaggct ttcaacaaat gttctaactg tcttatttgt gacctcactt tttatatttt
     6421 tttaattttt aaaaatatta gaagtttcta ggataatttt ttcgactttt attctctcta
     6481 ccgtccgcac tcttcttact tttaaattaa attgtttttt tttcagttgg gaaacacttt
     6541 gctcacaagt ttgtacaaaa aagcaggctc aatggtgtca aagggcgaag agctgtttac
     6601 tggagtcgtc ccaattctgg tggagctgga tggagatgtc aacgggcata agttttcagt
     6661 gcgaggagag ggagaaggag acgctaccaa cggcaagctg acactgaaat tcatcagcac
     6721 cacagggaag ctgcccgtgc cttgggtaag tttaaacata tatatactaa ctaaccctga
     6781 ttatttaaat tttcagccaa ccctggtcac taccctgagt tggggcgtcc agtcatttgc
     6841 ccgataccca gaccacatga agcagcatga tttctttaaa tccgctatgc ccgagggcta
     6901 cgtgcaggaa cggacaattt tctttaagga cgatggaaca tacaaaacta gtaagtttaa
     6961 acagttcggt actaactaac catacatatt taaattttca ggagcagagg tgaagttcga
     7021 aggagacact ctggtcaaca ggatcgagct gaagggcatt gactttaaag aagatggaaa
     7081 tatcctgggc cacaaactgg agtacaacgc catccatggc aacgtgtaca ttacagctga
     7141 taagcagaaa aacgggatta aggcaaattt cggactgaac tgcaatgtgg aagacgggag
     7201 tgtccagctg gccgatcact accagcagaa cactcctatc ggcgacgggc ccgtgctgct
     7261 gcctgataat cattatctga gcacccagtc cgtcctgtct aaggacccca atgagaaaag
     7321 agatcacatg gtgctgctgg agtttgtgac cgctgctggc attaccctgg ggatggacga
     7381 gctgtacaag ggaccaggcg agaatttgta ttttcagggt cgtcttgtca agttccttgt
     7441 tggaggacca ggcatggtgt ctaaaggcga ggaactgttc actggggtgg tgcccatcct
     7501 ggtggagctg gacggcgatg tgaatgggca caagtttagc gtgcgcggag agggagaagg
     7561 ggacgctacc aacggaaagc tgacactgaa actgatttcc accacaggca agctgcccgt
     7621 gccttggcca acactggtga ctaccctggg atacggcctc cagagcttcg cccggtatcc
     7681 agaccacatg aagcagcatg atttctttaa atcagctatg cccgagggct acgtgcagga
     7741 acggacaatc ttctttaagg acgatgggac atataaaact agagccgagg tgaagtttga
     7801 aggggacact ctggtgaaca ggatcgagct gaagggaatt gacttcaaag aagatggaaa
     7861 tatcctgggc cacaagctgg agtacaactt caacagccat aacgtgtata tcaccgccga
     7921 taagcagaaa aacggcatca aggctaattt caaaattagg cacaatgtgg aagacggcgg
     7981 ggtgcagctg gctgatcatt accagcagaa cacacctatc ggggacggac ccgtgctgct
     8041 gcctgataat cactacctga gctatcagtc cgtgctgtct aaggacccaa acgagaaacg
     8101 ggatcatatg gtgctgaaag aatttgtgac tgccgctggg attaccctgg gaatggatga
     8161 gctgtataag cacccagctt tcttgtacaa agtgggaatg aagctctccc cagctaccaa
     8221 gtctttcatc ggtaagacag tcgatatttc cacatttgca atccaatggg gattcgtgcc
     8281 gtttgtggtc taccttggat ttaagaaagg cgctgagcca atgccaaatg gacaggtaaa
     8341 aactagaaaa agaaacattt agttttaaaa tcacgtctcc gagagagcgt aataaataaa
     8401 aaatgcgtcc cataatacac aagattcaaa catttatata gataccgcat tttctttaca
     8461 agtttctcgt tgtgtaaata gaacaattgc atgcgagacg ctgccaatta ttttttaatt
     8521 tatttgtcaa aagtttaagt tttaccaaaa acacctatgc tttcaattca aagatttaag
     8581 aattcatatt tcagatcctc cctctttccg ccatgagtct tctctgggga tagtttatct
     8641 acgaaagcag ccgggtggcc gttggatcgt tctgaatctc atagttttgt tagcctccac
     8701 tcattgtttt cttcttttat taataaacag ttaaagtttc cttgccaact ttattataca
     8761 tagttgataa ttcactggcc gtcgttttac actcgagacg tacggtgcgc gcgatgcatt
     8821 cgaagatctg cccactagtg agtcgtatta taagtgcaag taagatcagt gtttgtttcg
     8881 atatcagtct gtttcgtaac ggtcttctgt ataactacaa aaaagaataa aaaaccgtat
     8941 cacaactgag aaaaaagaca aaacttattg aacagtgttc ctttcaaaaa attattttgt
     9001 aaaaaatcga gtttttctcc aaaataacgc acgcaatcaa aaacaagaaa actaacgtgt
     9061 tctcctgggc gtggaactcc ttatcaagat taccattatt gtctgtcaga attgatattt
     9121 caattatttc atatctagac aaacccgggt gtcttgcatt tattagtact gtgagtttac
     9181 atacaaacga ggcttactca gataacatag agtcacctct aattagcaaa aacccatttc
     9241 taattagcta tttcgaccag ttctaaagtc accgtaatta gaaatggttt ttgtagcttt
     9301 tctaattaga aatgaccccg agataagtga aatatggcat tttctgggtt ttctagtaag
     9361 aaatgatccc gcacgtagtg attccgcgtt acaagccaaa ttcaaattaa aaaagtatat
     9421 tacacaattc aagttacatt attttcagga aacaatggcg aagagcaaag cagagattca
     9481 aaaggaggaa agagacaaca gatatgaacg aagacttctt gaaaagagaa agagacatga
     9541 ggtgagtaac catgataata aataatcaag taagggctat agatttataa attctggagt
     9601 taacagtttc gaaaaaaagt agttttcata tgtgcgaagg aatatcaaaa ataggggaat
     9661 agcctaaacg agtgagaaaa ttaaatgttt gttatttttt caaaaatgtc tgcagggccg
     9721 ttatcctttc aaatgcactc tatcaaacat agatcatatc catcatatct tatccagaat
     9781 ttatatcata tccaagattg agagcgtttc aaggaaaatc tcaaacaacc atacaatttt
     9841 gtagatcgac aacaaggcct ccacttcttc atcgatggcc tctgcagcac ctgcaccacc
     9901 aagtttgtat ttttcatatt aatataaagt gagccacgca tagtcgtttt taggattttt
     9961 gccagatcga tgtcgcgcaa acagactgtt tttttttctt tgaaagccgt gcgtgacatg
    10021 ggccacggat tgtaacgccg cgctggatgg ataaaaattt attcagtttt ttagccttta
    10081 caaaatatgg ttcgactact ttctttattt ttacattttt ataggtgctc attgagctga
    10141 gatattttgg gtataatcgg aaaatcgctg attttggtca tttttctgca ctaaagatta
    10201 cacaaaaagt tcagtattta gattcattgt tgaaaattgg ggatgatcta cagatacata
    10261 tcgccgtgtt caaaaaca
//
