LOCUS       sequence-122833-        8362 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  The prostate specific promoter controls expression of FlpO linked to
            a blue flourescent protein. gRNA towards PTEN ex5, p53 ex7 and 8, 
            and SMAD4 ex2 and ex 9 are expressed by the U6 promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8362)
  TITLE     Cas9/CRISPR pig transposon 
            plasmid_LIR_TRE_CAG_mKateII-Puro-STOP_mVenus-Cas9-PA-RIR
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8362)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: 5xgRNA: PTEN exon 5, p53 exon 8, SMAD4 exon 2,...
FEATURES             Location/Qualifiers
     source          1..8362
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     364..386
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     378..395
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        698..946
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     misc_RNA        967..1042
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        1055..1303
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     misc_RNA        1324..1399
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        1412..1660
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     misc_RNA        1681..1756
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        1769..2017
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     misc_RNA        2038..2113
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        2126..2374
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     misc_RNA        2395..2470
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     regulatory      2970..2979
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2976..2978,2979..2981,2982..3692)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Goedhart et 
                     al., 2012)"
                     /label=mTurquoise2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYFSDNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3021..3042)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3282..3301)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3629..3650
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             3708..3764
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             join(3771..3776,3777..3797,3798..5069)
                     /codon_start=1
                     /product="nuclear-targeted site-specific recombinase"
                     /label=FLPo
                     /note="FLPo is a mouse codon-optimized fusion between the 
                     SV40 nuclear localization signal and a thermostable version
                     of the Saccharomyces cerevisiae site-specific recombinase 
                     FLP.  Recombination occurs at FRT sequences."
                     /translation="MAPKKKRKVMSQFDILCKTPPKVLVRQFVERFERPSGEKIASCAA
                     ELTYLCWMITHNGTAIKRATFMSYNTIISNSLSFDIVNKSLQFKYKTQKATILEASLKK
                     LIPAWEFTIIPYNGQKHQSDITDIVSSLQLQFESSEEADKGNSHSKKMLKALLSEGESI
                     WEITEKILNSFEYTSRFTKTKTLYQFLFLATFINCGRFSDIKNVDPKSFKLVQNKYLGV
                     IIQCLVTETKTSVSRHIYFFSARGRIDPLVYLDEFLRNSEPVLKRVNRTGNSSSNKQEY
                     QLLKDNLVRSYNKALKKNAPYPIFAIKNGPKSHIGRHLMTSFLSMKGLTELTNVVGNWS
                     DKRASAVARTTYTHQITAIPDHYFALVSRYYAYDPISKEMIALKDETNPIEEWQHIEQL
                     KGSAEGSIRYPAWNGIISQEVLDYLSSYINRRI"
     polyA_signal    5173..5294
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5210..5229)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(5378..5395)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5384..5608
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    complement(5625..5658)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     primer_bind     complement(6141..6157)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6141..6157)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6154..6176)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6165..6181
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6189..6195,6196..6213,6214..6219))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6234..6255
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6372..6389)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6543..7131)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6623..6642)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7302..8093,8094..8162))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7925..7944
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8163..8267)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8335..8353
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cagatctgat atctctagag
      421 aagtcgacgt ttaaagtacc ctacagttga agtcggaagt ttacatacac ttaagttgga
      481 gtcattaaaa ctcgtttttc aactactcca caaatttctt gttaacaaac aatagttttg
      541 gcaagtcagt taggacatct actttgtgca tgacacaagt catttttcca acaattgttt
      601 acagacagat tatttcactt ataattcact gtatcacaat tccagtgggt cagaagttta
      661 catacactaa gttgactgtg cctttaaaca gtcgcgagag ggcctatttc ccatgattcc
      721 ttcatatttg catatacgat acaaggctgt tagagagata attggaatta atttgactgt
      781 aaacacaaag atattagtac aaaatacgtg acgtagaaag taataatttc ttgggtagtt
      841 tgcagtttta aaattatgtt ttaaaatgga ctatcatatg cttaccgtaa cttgaaagta
      901 tttcgatttc ttggctttat atatcttgtg gaaaggacga aacaccgcag caattcactg
      961 taaagcgttt tagagctaga aatagcaagt taaaataagg ctagtccgtt atcaacttga
     1021 aaaagtggca ccgagtcggt gcttttttac cggtgagggc ctatttccca tgattccttc
     1081 atatttgcat atacgataca aggctgttag agagataatt ggaattaatt tgactgtaaa
     1141 cacaaagata ttagtacaaa atacgtgacg tagaaagtaa taatttcttg ggtagtttgc
     1201 agttttaaaa ttatgtttta aaatggacta tcatatgctt accgtaactt gaaagtattt
     1261 cgatttcttg gctttatata tcttgtggaa aggacgaaac accgtgcgtg tttgtgcctg
     1321 tccgttttag agctagaaat agcaagttaa aataaggcta gtccgttatc aacttgaaaa
     1381 agtggcaccg agtcggtgct tttttatcga tgagggccta tttcccatga ttccttcata
     1441 tttgcatata cgatacaagg ctgttagaga gataattgga attaatttga ctgtaaacac
     1501 aaagatatta gtacaaaata cgtgacgtag aaagtaataa tttcttgggt agtttgcagt
     1561 tttaaaatta tgttttaaaa tggactatca tatgcttacc gtaacttgaa agtatttcga
     1621 tttcttggct ttatatatct tgtggaaagg acgaaacacc gaaggagaaa aaagatgaat
     1681 gttttagagc tagaaatagc aagttaaaat aaggctagtc cgttatcaac ttgaaaaagt
     1741 ggcaccgagt cggtgctttt ttggtaccga gggcctattt cccatgattc cttcatattt
     1801 gcatatacga tacaaggctg ttagagagat aattggaatt aatttgactg taaacacaaa
     1861 gatattagta caaaatacgt gacgtagaaa gtaataattt cttgggtagt ttgcagtttt
     1921 aaaattatgt tttaaaatgg actatcatat gcttaccgta acttgaaagt atttcgattt
     1981 cttggcttta tatatcttgt ggaaaggacg aaacaccgtg caacagctcc tgcatgggtt
     2041 ttagagctag aaatagcaag ttaaaataag gctagtccgt tatcaacttg aaaaagtggc
     2101 accgagtcgg tgcttttttg agctcgaggg cctatttccc atgattcctt catatttgca
     2161 tatacgatac aaggctgtta gagagataat tggaattaat ttgactgtaa acacaaagat
     2221 attagtacaa aatacgtgac gtagaaagta ataatttctt gggtagtttg cagttttaaa
     2281 attatgtttt aaaatggact atcatatgct taccgtaact tgaaagtatt tcgatttctt
     2341 ggctttatat atcttgtgga aaggacgaaa caccgtcaac tctccaatgt ccacgtttta
     2401 gagctagaaa tagcaagtta aaataaggct agtccgttat caacttgaaa aagtggcacc
     2461 gagtcggtgc ttttttctcg agactagtta tgatagcatc ttgttcttag tctttttctt
     2521 aatagggaca taaagcccac aaataaaaat atgcctgaag aatgggacag gcattgggca
     2581 ttgtccatgc ctagtaaagt actccaagaa cctatttgta tactagatga cacaatgtaa
     2641 tagtcatcat gtttaaacat ctaccattcc agttaagaaa atatgatagc atcttgttct
     2701 tagtcttttt cttaataggg acataaagcc cacaaataaa aatatgcctg aagaatggga
     2761 caggcattgg gcattgtcca tgcctagtaa agtactccaa gaacctattt gtatactaga
     2821 tgacacaatg tcaatgtctg tgtacaactg ccaactggga tgcaagacac tgcccatgcc
     2881 aatcatcctg aaaagcagct ataaaaagca ggaagctact ctgcaccttg tcagtgaggt
     2941 ccagatacct acagattaat taacccgggg ccaccatggt gagcaagggc gaggagctgt
     3001 tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca
     3061 gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct
     3121 gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg tcctggggcg
     3181 tgcagtgctt cgcccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca
     3241 tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga
     3301 cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca
     3361 tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac tactttagcg
     3421 acaacgtcta tatcaccgcc gacaagcaga agaacggcat caaggccaac ttcaagatcc
     3481 gccacaacat cgaggacggc ggcgtgcagc tcgccgacca ctaccagcag aacaccccca
     3541 tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag tccaagctga
     3601 gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg
     3661 ggatcactct cggcatggac gagctgtaca aggtatacgg aagcggagct actaacttca
     3721 gcctgctgaa gcaggctgga gacgtggagg agaaccctgg acctgctagc atggctccta
     3781 agaagaagag gaaggtgatg agccagttcg acatcctgtg caagaccccc cccaaggtgc
     3841 tggtgcggca gttcgtggag agattcgaga ggcccagcgg cgagaagatc gccagctgtg
     3901 ccgccgagct gacctacctg tgctggatga tcacccacaa cggcaccgcc atcaagaggg
     3961 ccaccttcat gagctacaac accatcatca gcaacagcct gagcttcgac atcgtgaaca
     4021 agagcctgca gttcaagtac aagacccaga aggccaccat cctggaggcc agcctgaaga
     4081 agctgatccc cgcctgggag ttcaccatca tcccttacaa cggccagaag caccagagcg
     4141 acatcaccga catcgtgtcc agcctgcagc tgcagttcga gagcagcgag gaggccgaca
     4201 agggcaacag ccacagcaag aagatgctga aggccctgct gtccgagggc gagagcatct
     4261 gggagatcac cgagaagatc ctgaacagct tcgagtacac cagcaggttc accaagacca
     4321 agaccctgta ccagttcctg ttcctggcca cattcatcaa ctgcggcagg ttcagcgaca
     4381 tcaagaacgt ggaccccaag agcttcaagc tggtgcagaa caagtacctg ggcgtgatca
     4441 ttcagtgcct ggtgaccgag accaagacaa gcgtgtccag gcacatctac tttttcagcg
     4501 ccagaggcag gatcgacccc ctggtgtacc tggacgagtt cctgaggaac agcgagcccg
     4561 tgctgaagag agtgaacagg accggcaaca gcagcagcaa caagcaggag taccagctgc
     4621 tgaaggacaa cctggtgcgc agctacaaca aggccctgaa gaagaacgcc ccctacccca
     4681 tcttcgctat caagaacggc cctaagagcc acatcggcag gcacctgatg accagctttc
     4741 tgagcatgaa gggcctgacc gagctgacaa acgtggtggg caactggagc gacaagaggg
     4801 cctccgccgt ggccaggacc acctacaccc accagatcac cgccatcccc gaccactact
     4861 tcgccctggt gtccaggtac tacgcctacg accccatcag caaggagatg atcgccctga
     4921 aggacgagac caaccccatc gaggagtggc agcacatcga gcagctgaag ggcagcgccg
     4981 agggcagcat cagatacccc gcctggaacg gcatcatcag ccaggaggtg ctggactacc
     5041 tgagcagcta catcaacagg cggatctaaa tgcatcagcc ataccacatt tgtagaggtt
     5101 ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca
     5161 attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
     5221 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
     5281 atcaatgtat cttaacgcgt aaattgtaag cgggccggcc atatttgtca ccggactgcg
     5341 gccgcgactc tagatcataa tcagcccggg ggatcagcct cgactgtgcc ttctagttgc
     5401 cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc
     5461 actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct
     5521 attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg
     5581 catgctgggg atgcggtggg ctctatggaa ccagctgggg cgcgataact tcgtatagga
     5641 gactttatac gaagttatgc gccattctag ttgtggtttg tccaaactca tcaatgtatc
     5701 ttatcatgtc tggatcccat cacaaagctc tgacctcaat cctatagaaa ggaggaatga
     5761 gccaaaattc acccaactta ttgtgggaag cttgtggaag gctactcgaa atgtttgacc
     5821 caagttaaac aatttaaagg caatgctacc aaatactaat tgagtgtatg ttaacttctg
     5881 acccactggg aatgtgatga aagaaataaa agctgaaatg aatcattctc tctactatta
     5941 ttctgatatt tcacattctt aaaataaagt ggtgatccta actgacctta agacagggaa
     6001 tctttactcg gattaaatgt caggaattgt gaaaaagtga gtttaaatgt atttggctaa
     6061 ggtgtatgta aacttccgac ttcaactgta ggggatcctc tagagtcgac ctgcaggcat
     6121 gcaagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
     6181 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
     6241 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
     6301 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
     6361 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     6421 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     6481 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     6541 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     6601 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     6661 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     6721 agcgtggcgc tttctcaatg ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     6781 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     6841 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     6901 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     6961 cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt
     7021 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     7081 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     7141 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     7201 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     7261 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     7321 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     7381 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     7441 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     7501 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     7561 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     7621 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     7681 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     7741 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     7801 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     7861 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     7921 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     7981 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     8041 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     8101 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     8161 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     8221 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     8281 aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg
     8341 cgtatcacga ggccctttcg tc
//
