LOCUS       sequence-122843-         898 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lgg-1 tagged with oxCerulean-oxVenus (dFP, or double fluorescent 
            protein), expressed in the pharynx, for monitoring autophagy.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 898)
  AUTHORS   Chapin HA, Okada M, Merz AJ, Miller DL
  TITLE     Tissue-specific autophagy responses to aging and stress in C. 
            elegans.
  JOURNAL   Aging (Albany NY). 2015 Jun 30.
  PUBMED    26142908
REFERENCE   2  (bases 1 to 898)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Aging 
            (Albany NY). 2015 Jun 30."
COMMENT     Sequence Label: pMyo-2::oxCerulean-oxVenus::Lgg-1
FEATURES             Location/Qualifiers
     source          1..898
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    725..749
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gtgtcttctt cgtttattca tgaatcaaaa atttgaaagt aatagcaaaa aaatatcaaa
       61 atccgagaga aaacatgatt ttctgggagg ggagaagagc aacttcgtag acaatagttt
      121 gaatagaagt cactactcgg aaccgtcata cgagaaaaat gagaagaaaa tgtgaaggaa
      181 atacagaatt attacagaaa atgagaagat ttttttaaag gggctttggg tttccattag
      241 aagtatgaaa tttccattgt acaagtatac acattcgtcg gcggataata catgacactt
      301 tattccttct tttcgacctc tcctccatac acactttcgt cactgtaggc gatgtaaagg
      361 aacaagtctt cctcgtgatg gtcctgtaat aaagaaaagg gttaattaaa tgaaaataaa
      421 cgactggtta gttacctggt agagttgtcc cattgtggtc atggtttgtg gaatgacatt
      481 gttgacaaag aagaacagag catcttctgg acgaagttgg atgcgttttc tgatgaggaa
      541 gtagaactgt ccaacagtaa gatcggatgg gaccaagtac ttcttcttat ccaagtcatg
      601 gagctttgac tttggtgctt tctcaacaat cactggaata cggtctgggt actttctgcg
      661 gatcttgtct ccttcggcac gacgcttctc aaagttgttc tcctccttgt aagcccactt
      721 cattcccact ttgtacaaga aagctgggtg cttatacagc tcatccattc ccagggtaat
      781 cccagcggca gtcacaaatt ctttcagcac catatgatcc cgtttctcgt ttgggtcctt
      841 agacagcacg gactgatagc tcaggtagtg attatcaggc agcagcacgg gtccgtcc
//
