LOCUS       sequence-122898-         855 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPaint frame selector +1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 855)
  AUTHORS   Schmid-Burgk JL, Honing K, Ebert TS, Hornung V
  TITLE     CRISPaint allows modular base-specific gene tagging using a 
            ligase-4-dependent mechanism.
  JOURNAL   Nat Commun. 2016 Jul 28;7:12338. doi: 10.1038/ncomms12338.
  PUBMED    27465542
REFERENCE   2  (bases 1 to 855)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-07-28"; volume: "7:12338. doi"; pages: " 
            10.1038/ncomms12338"
COMMENT     Sequence Label: pCAS9-mCherry-Frame +1
FEATURES             Location/Qualifiers
     source          1..855
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             274..327
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
ORIGIN
        1 cggatgtacc ccgaggacgg cgccctgaag ggcgagatca agcagaggct gaagctgaag
       61 gacggcggcc actacgacgc tgaggtcaag accacctaca aggccaagaa gcccgtgcag
      121 ctgcccggcg cctacaacgt caacatcaag ttggacatca cctcccacaa cgaggactac
      181 accatcgtgg aacagtacga acgcgccgag ggccgccact ccaccggcgg catggacgag
      241 ctgtacaagg tcgctaccat cgagggatcc ggagagggca gaggaagtct gctaacatgc
      301 ggtgacgtcg aggagaatcc tggcccaatg gacaagaagt acagcatcgg cctggacatc
      361 ggcaccaaca gcgtgggctg ggccgtgatc accgacgagt acaaggtgcc cagcaagaag
      421 ttcaaggtgc tgggcaacac cgaccgccac agcatcaaga agaacctgat cggcgccctg
      481 ctgttcgaca gcggcgagac cgccgaggcc acccgcctga agcgcaccgc ccgccgccgc
      541 tacacccgcc gcaagaaccg catctgctac ctgcaggaga tcttcagcaa cgagatggcc
      601 aaggtggacg acagcttctt ccaccgcctg gaggagagct tcctggtgga ggaggacaag
      661 aagcacgagc gccaccccat cttcggcaac atcgtggacg aggtggccta ccacgagaag
      721 taccccacca tctaccacct gcgcaagaag ctggtggaca gcaccgacaa ggccgacctg
      781 cgcctgatct acctggccct ggcccacatg atcaagttcc gcggccactt cctgatcgag
      841 ggcgacctga acccc
//
