LOCUS       sequence-122902-         850 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPaint frame selector +2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 850)
  AUTHORS   Schmid-Burgk JL, Honing K, Ebert TS, Hornung V
  TITLE     CRISPaint allows modular base-specific gene tagging using a 
            ligase-4-dependent mechanism.
  JOURNAL   Nat Commun. 2016 Jul 28;7:12338. doi: 10.1038/ncomms12338.
  PUBMED    27465542
REFERENCE   2  (bases 1 to 850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-07-28"; volume: "7:12338. doi"; pages: " 
            10.1038/ncomms12338"
COMMENT     Sequence Label: pCAS9-mCherry-Frame +2
FEATURES             Location/Qualifiers
     source          1..850
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             271..324
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
ORIGIN
        1 atgtaccccg aggacggcgc cctgaagggc gagatcaagc agaggctgaa gctgaaggac
       61 ggcggccact acgacgctga ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg
      121 cccggcgcct acaacgtcaa catcaagttg gacatcacct cccacaacga ggactacacc
      181 atcgtggaac agtacgaacg cgccgagggc cgccactcca ccggcggcat ggacgagctg
      241 tacaaggtcg ctaccatcga gggatccgga gagggcagag gaagtctgct aacatgcggt
      301 gacgtcgagg agaatcctgg cccaatggac aagaagtaca gcatcggcct ggacatcggc
      361 accaacagcg tgggctgggc cgtgatcacc gacgagtaca aggtgcccag caagaagttc
      421 aaggtgctgg gcaacaccga ccgccacagc atcaagaaga acctgatcgg cgccctgctg
      481 ttcgacagcg gcgagaccgc cgaggccacc cgcctgaagc gcaccgcccg ccgccgctac
      541 acccgccgca agaaccgcat ctgctacctg caggagatct tcagcaacga gatggccaag
      601 gtggacgaca gcttcttcca ccgcctggag gagagcttcc tggtggagga ggacaagaag
      661 cacgagcgcc accccatctt cggcaacatc gtggacgagg tggcctacca cgagaagtac
      721 cccaccatct accacctgcg caagaagctg gtggacagca ccgacaaggc cgacctgcgc
      781 ctgatctacc tggccctggc ccacatgatc aagttccgcg gccacttcct gatcgagggc
      841 gacctgaacc
//
