LOCUS       sequence-122914-        1091 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expression of gRNA under control of the Drosophila U6:3 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1091)
  AUTHORS   Port F, Chen HM, Lee T, Bullock SL
  TITLE     Optimized CRISPR/Cas tools for efficient germline and somatic genome
            engineering in Drosophila.
  JOURNAL   Proc Natl Acad Sci U S A. 2014 Jul 7. pii: 201405500.
  PUBMED    25002478
REFERENCE   2  (bases 1 to 1091)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2014 Jul 7. pii: 201405500."
COMMENT     Sequence Label: pCFD3-dU6:3gRNA
FEATURES             Location/Qualifiers
     source          1..1091
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     23..43
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        25..43
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_RNA        complement(354..429)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(448..868)
                     /label=dU6-3 promoter
                     /note="RNA polymerase III promoter for Drosophila U6-3 
                     snRNA (Port et al., 2014)"
ORIGIN
        1 tgaccatgat tacgccaagc gcgcaattaa ccctcactaa agggaacaaa agctggagct
       61 cctgcaggtt gttggttggc acaccacaaa tatactgttg ccgagcacaa ttgtctagaa
      121 tgcatacgca ttaagcgaac attaaaaaga tgtgaaaaca taactattat gtctaaataa
      181 acacacgtca gatgtatgta cgtcaacgga aaaccattgt ctatatatta caattactaa
      241 atacatacca aattgaatac atattgatga aaaataataa atactggcga aagcaaaaaa
      301 acgaaacatt tttattttat tgaacaactc tcaggctcca ggtaggcaaa aaagcaccga
      361 ctcggtgcca ctttttcaag ttgataacgg actagcctta ttttaacttg ctatttctag
      421 ctctaaaaca ggtcttctcg aagaccccga cgttaaattg aaaataggtc tatatatacg
      481 aactgagtct ggaaaaagaa gttgagaatt ataaaaagta gtgagcactg gcgccctctc
      541 tgcttggcga gctaaccttt tcgcctcttg gctgagtagg tggcgtttca ttctactctg
      601 taaaattaat gtagaattga aacactatgg tcaaaaaata cttaggggca taagttataa
      661 aacgtatgaa atgaattttt atcaacctgg gctattcaaa aattttcgaa ttattttatg
      721 tatttttttt aatcgttttt cattataggt taaaatacac tttaaaagga attctttcct
      781 gtaaaataaa tataaataaa tatgctttat tgacagaaaa tttgatgttt ttgtatttga
      841 gtaggagcaa tcacaggtga gcaaaaaaga attcagatct caattgatcg gctaaatggt
      901 atggcaagaa aaggtatgca atataataat cttttattgg gtatgcaacg aaaatttgtt
      961 tcgtcaacgt atgcaatatt ctttattaaa agagggtatg caatgtattt tattaaaaac
     1021 gggtatgcaa tataataatc ttttattggg tatgcaacga aaatttgttt cgtcaaagta
     1081 tgcaatattt t
//
