LOCUS       sequence-122921-         850 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Alpha Actinin, Excitation: 505, Emission: 515.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 850)
  AUTHORS   Lam AJ, St-Pierre F, Gong Y, Marshall JD, Cranfill PJ, Baird MA, 
            McKeown MR, Wiedenmann J, Davidson MW, Schnitzer MJ, Tsien RY, Lin 
            MZ
  TITLE     Improving FRET dynamic range with bright green and red fluorescent 
            proteins.
  JOURNAL   Nat Methods. 2012 Sep 9. doi: 10.1038/nmeth.2171.
  PUBMED    22961245
REFERENCE   2  (bases 1 to 850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2012 Sep 9. doi: 10.1038/nmeth.2171."
COMMENT     Sequence Label: Clover-Alpha-Actinin-19
FEATURES             Location/Qualifiers
     source          1..850
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      8..17
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(112..315)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(141..161)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        316..619
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 ttgctcacca tggtggcgac cggtgcggcg acggccaggc cgaggaggcc gagcagcaac
       61 ggcacggata gcagcatggt ggcgctagcg gatctgacgg ttcactaaac cagctctgct
      121 tatatagacc tcccaccgta cacgcctacc gcccatttgc gtcaatgggg cggagttgtt
      181 acgacatttt ggaaagtccc gttgattttg gtgccaaaac aaactcccat tgacgtcaat
      241 ggggtggaga cttggaaatc cccgtgagtc aaaccgctat ccacgcccat tgatgtactg
      301 ccaaaaccgc atcaccatgg taatagcgat gactaatacg tagatgtact gccaagtagg
      361 aaagtcccat aaggtcatgt actgggcata atgccaggcg ggccatttac cgtcattgac
      421 gtcaataggg ggcgtacttg gcatatgata cacttgatgt actgccaagt gggcagttta
      481 ccgtaaatac tccacccatt gacgtcaatg gaaagtccct attggcgtta ctatgggaac
      541 atacgtcatt attgacgtca atgggcgggg gtcgttgggc ggtcagccag gcgggccatt
      601 taccgtaagt tatgtaacgc ggaactccat atatgggcta tgaactaatg accccgtaat
      661 tgattactat taataactaa tgcatggcgg taatacggtt atccacagaa tcaggggata
      721 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
      781 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
      841 caagtcagag
//
