LOCUS       sequence-122989-         358 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Constitutive selectable expression of C-term V5 tagged proteins.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 358)
  AUTHORS   McHugh CA, Chen CK, Chow A, Surka CF, Tran C, McDonel P, 
            Pandya-Jones A, Blanco M, Burghard C, Moradian A, Sweredoski MJ, 
            Shishkin AA, Su J, Lander ES, Hess S, Plath K, Guttman M
  TITLE     The Xist lncRNA interacts directly with SHARP to silence 
            transcription through HDAC3.
  JOURNAL   Nature. 2015 May 14;521(7551):232-6. doi: 10.1038/nature14443. Epub 
            2015 Apr 27.
  PUBMED    25915022
REFERENCE   2  (bases 1 to 358)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature14443"; journalName: "Nature"; date: "2015-05-14- 
            14"; volume: "521"; issue: "7551"; pages: "232-6"
COMMENT     Sequence Label: pCAG-GW-V5-Hygro
FEATURES             Location/Qualifiers
     source          1..358
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(131..255)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             263..304
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 gtctccgtta tcggggaaga agtggctgat ctcagccacc gcgaaaatga catcaaaaac
       61 gccattaacc tgatgttctg gggaatataa atgtcaggct cccttataca cagccagtct
      121 gcaggtcgac catagtgact ggatatgttg tgttttacag tattatgtag tctgtttttt
      181 atgcaaaatc taatttaata tattgatatt tatatcattt tacgtttctc gttcagcttt
      241 cttgtacaaa gtggttgata tcggtaagcc tatccctaac cctctcctcg gtctcgattc
      301 tacgtagtaa tgagctcgat aagcttgata tcgaattccg cccccccccc ctctccct
//
